Ischemic stroke was induced in female C57BL/6JRj mice by a transient middle cerebral artery occlusion (tMCAo), which is mimicking one of the most common types of ischemic stroke in patients (Engel et al., 2011 (link)). The intraluminal occlusion model was performed as described elsewhere with minor modifications (Engel et al., 2011 (link); Zinnhardt et al., 2015 (link)). In short, a 7–0 monofilament (tip diameter 190–200 ml, coating length 2–3 mm, 70SPRePK5, Doccol Corp., Sharon, MA, United States) was inserted in the right common carotid artery and placed to block blood supply via the middle cerebral artery. The filament was held in place for 30 min followed by retraction of the filament leading to reperfusion. A Laser Doppler probe (moorVMS-LDF2, Moor Instruments, United Kingdom) was placed on the skull of the mice to monitor cerebral blood flow, considering a drop of ≥ 80% CBF as a successful stroke induction. Animals were anesthetized during the whole time of surgery, using 1.5% isoflurane (Abbott Animal Health, AbbottPark, IL, United States) in a 2:1 air and oxygen mixture.
70sprepk5
Manufactured by Doccol
Sourced in United States
The 70SPRePK5 is a multi-purpose laboratory equipment. It is designed for general scientific applications. The core function of this product is to facilitate various laboratory procedures and experiments.
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Lab products found in correlation
2 protocols using 70sprepk5
1
Transient Middle Cerebral Artery Occlusion in Mice
2
Transient Focal Cerebral Ischemia in Mice
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In all, N=63, 3 to 4 months old, male C57BL/6 mice (22 to 25 g body weight) underwent right tMCAo (30 minutes), using an intraluminal occlusion model as described elsewhere with minor modifications.12 (link) In short, mice were anesthetized with 1.5% isoflurane (Abbott Animal Health,Abbott Park, IL, USA) in 100% O2. After preparing the right external common carotid artery, a 7-0 monofilament (tip diameter 190 to 200 μm, coating length 2 to 3 mm, 70SPRePK5, Doccol Corp., Sharon, MA, USA), was inserted in the external common carotid artery and positioned at the point where the MCA branches out. Successful occlusion was maintained for 30 minutes before retracting the filament allowing reperfusion. Reduction in blood flow was monitored intraoperatively via a Laser Doppler probe (Periflux 5000, Perimed Instruments, Järfälla, Sweden) attached to the skull of the mouse (Supplementary Figure 2 ). In the sham operated animals, the filament was shortly inserted into the external common carotid artery and immediately retracted.
Mice were kept under 1.5% isoflurane anesthesia in 100% oxygen throughout the procedure. Body temperature was maintained at physiologic level with a custom built heating pad.
Mice were kept under 1.5% isoflurane anesthesia in 100% oxygen throughout the procedure. Body temperature was maintained at physiologic level with a custom built heating pad.
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