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C cbl c 15

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C-Cbl (C-15) is a protein-coding gene that produces the Cbl proto-oncogene protein. This protein functions as an E3 ubiquitin-protein ligase, which is involved in the regulation of cell signaling pathways.

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Lab products found in correlation

P47phox, by Cell Signaling Technology (1 mentions) Cd11b, by BD (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (1 mentions) Horseradish peroxidase anti mouse, by Cell Signaling Technology (1 mentions) Py416 sfk, by Cell Signaling Technology (1 mentions) C raf ps259, by Cell Signaling Technology (1 mentions) Ahr h211, by Santa Cruz Biotechnology (1 mentions)

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C-Cbl

2 protocols using c cbl c 15

1

Cbl-b Plasmid Transduction in B Cells

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The cDNA encoding human Cbl-b and truncation mutants as well as human c-Cbl [20] (link) were subcloned into the plasmid MIGR1. Calcium Phosphate transfection of PlatE cells with MIGR1 was carried out as previously described [21] (link), [22] (link). Aliquots of cell lysates were immunoblotted to confirm the size of the expressed proteins. Cbl-b (H-454 and H-121) and c-Cbl (C-15) antibodies were from Santa Cruz Biotechnology. Wt and Cblb−/− B cells were transduced by resuspending in viral supernatant with 8 mg/ml polybrene in the presence of rIL-4 (50 ng/ml) and anti-CD40 (7.5 µg/ml) and centrifuging at 1000× g for 1.5 hr at room temperature. The cells were then washed and placed into fresh culture medium and allowed to grow for 48 hrs.
Veselits M., Tanaka A., Lipkowitz S., O'Neill S., Sciammas R., Finnegan A., Zhang J, & Clark M.R. (2014). Recruitment of Cbl-b to B Cell Antigen Receptor Couples Antigen Recognition to Toll-Like Receptor 9 Activation in Late Endosomes. PLoS ONE, 9(3), e89792.
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2

Antibody Panel for Flow Cytometry

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CD38 and CD11b for flow cytometry were from Becton Dickinson (Franklin Lakes, NJ). Lyn, Fgr, pY416-SFK, AhR, p47phox, mTOR, c-Raf pS259, c-Raf pS621, c-Raf pS289/296/301(c-Raf pC-terminal domain), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), horseradish peroxidase anti-mouse, and anti-rabbit antibodies were from Cell Signaling (Danvers, MA). Total c-Raf was from Becton Dickinson. c-Cbl (C-15) and AhR (H211) were from Santa Cruz Biotechnology (Santa Cruz, CA).
MacDonald R.J., Bunaciu R.P., Ip V., Dai D., Tran D., Varner J.D, & Yen A. (2018). Src family kinase inhibitor bosutinib enhances retinoic acid-induced differentiation of HL-60 leukemia cells. Leukemia & lymphoma, 59(12), 2941-2951.
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