The normal human epidermal keratinocyte (NHEK) cell line was obtained from PromoCell (Heidelberg, Germany) and grown in Keratinocyte Growth Medium 2 (PromoCell) supplemented with Keratinocyte Growth Medium 2 SupplementMix (PromoCell). All cells were maintained at 37°C in a humidified incubator with 5% CO2. The undifferentiated NHEK cells were treated with 2′-deoxy-5-azacytidine (DAC) (Sigma-Aldrich, St. Louis, MO, USA) for 72 h.
Keratinocyte growth medium 2 supplementmix
Manufactured by PromoCell
Sourced in Germany
Keratinocyte Growth Medium 2 SupplementMix is a laboratory product designed to support the growth and maintenance of keratinocytes in cell culture. The supplement mix contains a proprietary blend of essential growth factors and nutrients required for the optimal proliferation and differentiation of keratinocytes.
Automatically generated - may contain errors
2 protocols using keratinocyte growth medium 2 supplementmix
1
Epidermal Keratinocyte Demethylation
2
Epidermal Keratinocyte Differentiation Induction
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Normal human epidermal keratinocyte (NHEK) cells were obtained from the PromoCell (Heidelberg, Germany). NHEK cells were grown in Keratinocyte Growth Medium 2 (PromoCell) supplemented with Keratinocyte Growth Medium 2 SupplementMix (PromoCell). All cells were maintained at 37℃ in a humidified incubator at 5% CO2. We treated the undifferentiated NHEK cells with 2′-deoxy-5-azacytidine (DAC) for 72 hours and with trichostatin A (TSA) for 24 hours. DAC and TSA were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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