Tcnα was labeled using an NHS-Rhodamine fluorescent labeling kit (#46406, Thermo Fisher Scientific) following the manufacturer’s instructions. U-87 MG WT, LDLR‒/‒, and LRP1‒/‒ cells were incubated with 50 nM Rhodamine-labeled Tcnα in PBS for 30 min on ice. Cells were washed five times with ice-cold PBS and fixed with 4% paraformaldehyde (PFA) and the cell nuclei were labeled with Hoechst. Confocal images were captured with the Zeiss LSM 880 NLO with AiryScan System.
Nhs rhodamine fluorescent labeling kit
Manufactured by Thermo Fisher Scientific
The NHS-Rhodamine fluorescent labeling kit is a reagent designed to label primary amines on proteins, peptides, and other biomolecules with the rhodamine fluorophore. The kit provides a simple and efficient method for the covalent attachment of the rhodamine dye to target molecules, enabling their detection and visualization in various biological applications.
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Lab products found in correlation
2 protocols using nhs rhodamine fluorescent labeling kit
1
Rhodamine-labeled Tcnα Binding Assay
2
Rhodamine-labeled Tcnα Cellular Uptake
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Tcnα were labeled using an NHS-Rhodamine fluorescent labeling kit (#46406, Thermo Fisher Scientific) following the manufacturer’s instructions. HeLa or U-87 MG cells were incubated with 400 nM Rhodamine-labeled Tcnα in PBS for 30 min on ice, then washed three times with ice-cold PBS and incubated with the toxin-free medium at 37 °C for 10 min. Cells were washed with ice-cold PBS and subjected to immunofluorescence analysis.
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