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Paraformaldehyde (pfa)

Manufactured by Takara Bio
Sourced in Japan, China

Paraformaldehyde is a solid polymer of formaldehyde used as a fixative in histology and cell biology. It is a white, crystalline solid that is soluble in water and organic solvents. Paraformaldehyde is used to preserve biological samples for microscopy and other analytical techniques.

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3 protocols using paraformaldehyde (pfa)

1

Pure AST Evaluation in Preclinical Studies

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Pure AST was provided by Shanghai Yuanye Biotechnology Co., Ltd. For experiments, AST was dissolved in phosphate buffered saline (PBS) and diluted with normal saline. Pentobarbital sodium, papain and cysteine were purchased from Guangzhou Chemical General Factory, and hematoxylin, water-soluble eosin Y, toluidine blue O, paraformaldehyde and EDTA were acquired from Takara Bio, Inc. Anti-TNF (cat. no. ab1793), IL-1 (cat. no. ab9722), β-actin (cat. no. ab8227), AKT (cat. no. ab8805), NF-κB (cat. no. ab28849), PI3K (cat. no. ab140307) and IL-6 (cat. no. ab6672) antibodies were all purchased from Abcam. The quantitative PCR detection and Prime Script RT kits were purchased from Bao Biological Engineering Co. Ltd., and Takara Biotechnology Co. Ltd., respectively.
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2

Quantitative PCR and Immunohistochemistry Protocol

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Pure AST is provided by Shanghai Yuanye Biotechnology Co., Ltd. In the experiment, pure AST was dissolved with phosphate buffer (PBS) and diluted with saline. Pentobarbital sodium, papain, and cysteine were purchased from Guangzhou Chemical Reagent Factory. Hematoxylin, water-soluble eosin Y, toluidine blue O, paraformaldehyde, and Ethylene Diamine Tetraacetic Acid (EDTA) were from TAKARA, Japan. Anti- PEG2, β-actin, and other monoclonal antibodies were purchased from Abcam, UK. Finally, the immunohistochemical kit was purchased from Boster, Wuhan, China) The fluorescence quantitative PCR detection kit was purchased from Baoshi, Dalian, China, and the Prime Script RT kit were purchased from Takara, Dalian, China.
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3

Transwell-Based Cell Invasion Assay

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Transwell chamber (Millipore, Shijingshan, Haidian, USA) was applied for measuring cell invasion. Briefly, cells (0.5 mL; 2.5 × 104 cells) in the upper chamber were resuspended in serum-free RPMI1640 medium, while RPMI1640 medium in the lower chamber was supplemented with 10% FBS. After 48 h of incubation at 37 °C, cells attached to the lower surface were fixed with 4% paraformaldehyde (Takara Biotechnology, Dalian, China) and stained with 0.3% crystal violet solution (Takara Biotechnology, Dalian, China). Then, the invasion numbers were determined by photographed with an inverted microscope.
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