Hydromatrix

Manufactured by Agilent Technologies

Sourced in United Kingdom

Hydromatrix is a lab equipment product designed for sample preparation. It serves as a solid support material for various analytical techniques. The core function of Hydromatrix is to provide a consistent and controlled matrix for sample extraction, purification, and concentration.

Automatically generated - may contain errors

Lab products found in correlation

Ase 350, by Thermo Fisher Scientific (1 mentions) Accelerated solvent extractor, by Thermo Fisher Scientific (1 mentions) Vacuum oven, by Avantor (1 mentions) Laborota 4011, by Heidolph (1 mentions) Ase 150, by Thermo Fisher Scientific (1 mentions)

6 protocols using hydromatrix

Determination of POPs in Freeze-Dried Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were first freeze-dried, following the addition of 25 ng of each of 13 C-labeled BDE-47, BDE-99, BDE-153, BDE-209, TBBP-A, α-, β-and γ-HBCDs as internal (surrogate) standards.
Accurately weighed aliquots of the freeze-dried samples (~ 2 g) were loaded into pre-cleaned 66 mL Accelerated Solvent Extraction (ASE 300, Dionex Inc., UK) cells containing 1.5 g florisil, 3 g alumina, 5 g anhydrous Na 2 SO 4 and hydromatrix (Varian Inc., UK) to fill the void volume of the cells and spiked with 25 ng each of d 18 -α-HBCD and 13 C-BDE-154 as QA/QC standards to evaluate losses due to extraction and clean-up. The ASE cells were extracted with hexane:dichloromethane (1:9, v/v) at 90 ˚C and 1500 psi. The heating time was 5 minutes, static time 4 min, purge time 90 s, flush volume 50%, with three static cycles. The lipid weight of the studied samples was determined gravimetrically on separate aliquots using a standard procedure (European Standard EN 1528-2, 1996; see supporting information for a summary).

Harrad S, & Abdallah M.A. (2015). Concentrations of Polybrominated Diphenyl Ethers, Hexabromocyclododecanes and Tetrabromobisphenol-A in Breast Milk from United Kingdom Women Do Not Decrease over Twelve Months of Lactation. Environmental science & technology, 49(23).

+ Open protocol

+ Expand

Pressurized Liquid Extraction of Dust

Check if the same lab product or an alternative is used in the 5 most similar protocols

Accurately weighted aliquots of dust (~0.15 g) were loaded into pre-cleaned 66 mL cells containing 1.5 g Florisil and Hydromatrix (Varian Inc., UK) to fill the void volume of the cells, and spiked with internal (surrogate) standards (15 ng of each of BDE 77, BDE 128 and 30 ng of 13 C 12 -BDE 209) prior to pressurised liquid extraction (ASE 350, Dionex, Hemel Hempstead, UK) using hexane:dichloromethane (1:9, v/v) at 90 ˚C and 1500 psi. The heating time was 5 minutes, static time 4 min, purge time 90 s, flush volume 50 %, with three static cycles (Harrad and Abdallah, 2011) .

Harrad S., Abdallah M.A, & Oluseyi T. (2016). Polybrominated diphenyl ethers and polychlorinated biphenyls in dust from cars, homes, and offices in Lagos, Nigeria. Chemosphere, 146.

+ Open protocol

+ Expand

Lipid Extraction from Milk and Serum

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sheep serum and donated human breast milk were used for all method development and samples were stored at −40° C until extraction. Five grams of homogenized breast milk and one gram of serum were each spiked with a ¹³C- labeled surrogate mixture as shown in the schematic diagram of the method in Fig. 1. Isopropanol was added to the milk (5 mL) and serum (1 mL) to denature proteins, and then samples were mixed with 7 g of pre-washed Hydromatrix™ (Agilent Technologies, Santa Clara, CA). The extraction procedure was briefly modified based off a previously published method using pressurized liquid extraction with an accelerated solvent extractor (Dionex, Sunnyvale, CA) with 50/50 hexanes:dichloromethane (hex:DCM) at a temperature of 100° C, pressure of 1500 psi, 5 min cycle time performed 3 times, flush volume of 150%, and purge time of 100 seconds, resulting in an extraction volume of 120 mL [29 (link)]. Then, lipid content for breast milk was determined gravimetrically by evaporating the extracts down to dryness. Due to the small sample volume of serum, lipid content was determined by calculating total triglyceride and cholesterol levels enzymatically, presented previously by Schecter et al. [28 (link)].

Butryn D., Gross M., Chi L.H., Schecter A., Olson J, & Aga D. (2015). “One-shot” analysis of polybrominated diphenyl ethers and their hydroxylated and methoxylated analogs in human breast milk and serum using gas chromatography-tandem mass spectrometry. Analytica chimica acta, 892, 140-147.

+ Open protocol

+ Expand

Quantitative Analysis of Antibiotics

Check if the same lab product or an alternative is used in the 5 most similar protocols

Analytical grade SMX (≥ 98% purity) and TMP (≥ 98% purity) were purchased from Sigma-Aldrich (Oakville, ON, Canada). High purity (HPLC grade) methanol, acetic acid, acetone, and acetonitrile were purchased from Fisher Scientific (Ottawa, ON, Canada). Hydromatrix® was purchased from Agilent Technologies (Mississauga, ON, Canada). Selected physicochemical properties of the two antibiotics are listed in Table 2.1. Table 2.

Adesanya T., Zvomuya F, & Farenhorst A. (2020). Sulfamethoxazole sorption by cattail and switchgrass roots. Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes, 55(12).

+ Open protocol

+ Expand

Gravimetric Analysis of Freeze-Dried Zooplankton

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gravimetric analysis of the purchased freeze-dried zooplankton was performed using a Dionex Accelerated Solvent Extractor (ASE) 350 (Thermo Scientic, Waltham, MA) employing a modied version of the Folch method. 13, 35, 36 Approximately 100-200 mg of freeze-dried zooplankton powder was mixed with 5 mL of hydromatrix (Agilent, Santa Clara, CA). The mixture was compressed into a 10 mL ASE cell containing a cellulose lter (ASE 350 cell lter, Thermo Scientic). The remaining cell volume was lled with sand (Sigma, St. Louis, MO). Total lipids were extracted using a 2 : 1 (v/v) chloroform-methanol solution. Lipid extracts were washed with 0.88% (w/v) potassium chloride and 1 : 1 (v/v) methanol-water before concentrating to $1 mL with a Heidolph Laborota 4011 (Schwabach, Germany) rotary evaporator (40 C, 500 mmHg). Lipid extracts were transferred to pre-weighed aluminum pans, dried overnight in a vacuum oven (VWR, Radnor, PA) at 700 mmHg and room temperature, and weighed (AE0.00001 g). Prior to analysis, all glassware was baked at 400 C for 4 hours to remove any organic contamination.

Pinger C., Copeman L., Stowell M., Cormack B., Fugate C, & Rogers M. (2022). Rapid measurement of total lipids in zooplankton using the sulfo-phospho-vanillin reaction. Analytical methods : advancing methods and applications, 14(27).

+ Open protocol

+ Expand

Extraction and Analysis of Pharmaceuticals from Soil and Manure

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pharmaceuticals were extracted from soils and manure using a Dionex ASE 150 (Sunnyvale, CA, USA) PLE system. Samples were loaded into 22 mL stainless steel extraction cells as follows. An EDTA washed cellulose filter (1.9 mm pore size) followed by 1 g of diatomaceous earth was placed at the bottom of each cell. Five grams of the soil or manure sample mixed with 2 g diatomaceous earth was then added (Hydromatrix, Agilent Technologies, Inc., Palo Alto, CA, USA). The remaining cell void volume was filled with diatomaceous earth and another EDTA washed cellulose filter was placed on top. Extraction was carried out at 10 7 Pa and 40 C. Static extraction time was 20 min, with a flush volume of 60%, and purge volume of 60%. Two extraction cycles were performed using methanol:water (3:1, v/v) containing 0.25 mmol L ¡1 EDTA and 50 mmol L ¡1 sodium chloride at pH 8.0. Extracts were diluted with sufficient water to reduce the organic solvent concentration to less than 5% by volume. The solution pH was subsequently adjusted to 4.0 with 1 M HCl. Pharmaceuticals were extracted by solidphase extraction (SPE) as described below and analyzed via HPLC MS/MS.

Bair D.A., Popova I.E., Tate K.W, & Parikh S.J. (2017). Transport of oxytetracycline, chlortetracycline, and ivermectin in surface runoff from irrigated pasture. Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes, 52(9).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!