Optomotry hd

Mice (6-week-old C57BL/6; Koatech) were intravitreally injected with 2 µM BB-Cl-amidine after general anesthesia. Optomotor response measurement (OptoMotry HD; CerebralMechanics) and electroretinography (cat. no. UTAS E-2000; LKC Technologies) were performed 1 week after injection according to previous guidelines (26 (link)). Enucleated eyes were prepared and analyzed using H&E staining and TUNEL assay. Structure and toxicity evaluations were performed according to a previously established protocol (22 (link)). The results of H&E staining were analyzed by measuring the a/b ratio, where ‘a’ is the length from the innermost ganglion cell layer to the outermost inner nuclear layer, while ‘b’ is the length from the innermost ganglion cell layer to the outermost outer nuclear layer. All mice were maintained in a specific-pathogen free facility as previously described, in accordance with the ARVO statement.

The virtual optokinetic system (OptoMotry HD, CerebralMechanics) was used for measurement of the grating acuity as visual thresholds to drive head tracking of mice to a rotating virtual cylinder, according to the manufacturer’s instructions and original publications on the system.⁵¹ (link)^,52 (link) Briefly, mice were placed on a platform where they were exposed to a view of the rotating cylinder on the monitors. A staircase procedure was used for the determination of visual thresholds for the detection of the maximum spatial frequency (cycles/degrees) above which the mice did not respond to the rotating stimuli.