Animal erythrocytes were purified from whole blood of rabbit and sheep and stored in Alsever’s solution at +4 °C. Before use, they were washed with appropriate buffer: DGVB++ (dextrose gelatin veronal buffer with Ca2+ and Mg2+) for sheep erythrocytes (Esh) and GVB+ (gelatin veronal buffer with Mg2+) for rabbit erythrocytes (Erab). DGVB++ is 4.5 mM sodium barbital buffer containing 150 mM NaCl, 15 mM glucose, 0.15 mM CaCl2, 1 mM MgCl2, 0.05% gelatin; pH 7.4. GVB+ is 4.5 mM sodium barbital buffer containing 150 mM NaCl, 10 mM Mg-EGTA, 0.05% gelatin; pH 7.4. Before the experiments, Esh was sensitized with antibodies (anti-sheep red blood cell stroma antibodies produced in rabbits, S1389, Sigma, St. Louis, MO, USA) diluted 1:1600 for 40 min at +37 °C.
S1389
S1389 is a laboratory equipment product manufactured by Merck Group. It is a precision instrument designed for scientific research and analysis. The core function of S1389 is to perform accurate measurements and data collection, without further extrapolation on its intended use.
Lab products found in correlation
3 protocols using s1389
Complement Activation Assay with Erythrocytes
Animal erythrocytes were purified from whole blood of rabbit and sheep and stored in Alsever’s solution at +4 °C. Before use, they were washed with appropriate buffer: DGVB++ (dextrose gelatin veronal buffer with Ca2+ and Mg2+) for sheep erythrocytes (Esh) and GVB+ (gelatin veronal buffer with Mg2+) for rabbit erythrocytes (Erab). DGVB++ is 4.5 mM sodium barbital buffer containing 150 mM NaCl, 15 mM glucose, 0.15 mM CaCl2, 1 mM MgCl2, 0.05% gelatin; pH 7.4. GVB+ is 4.5 mM sodium barbital buffer containing 150 mM NaCl, 10 mM Mg-EGTA, 0.05% gelatin; pH 7.4. Before the experiments, Esh was sensitized with antibodies (anti-sheep red blood cell stroma antibodies produced in rabbits, S1389, Sigma, St. Louis, MO, USA) diluted 1:1600 for 40 min at +37 °C.
Complement System Activation Assays
Complement Activation Assay Procedure
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