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Humanref 8 v3

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The HumanRef-8 v3.0 is a high-throughput gene expression microarray platform developed by Illumina. It is designed to measure the expression levels of over 25,000 well-annotated human genes from a single sample. The platform utilizes Illumina's BeadArray technology to provide comprehensive and reliable gene expression data.

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Lab products found in correlation

Totalprep kit, by Illumina (1 mentions) Human ht 12 v4 beadarrays, by Illumina (1 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions) Beadstation array reader, by Illumina (1 mentions) Humanref 12 v 4 beadchip, by Illumina (1 mentions) Infinium 27k, by Illumina (1 mentions)

Spelling variants of this product

HumanRef-8 v3.0 expression beadchip (28 citations) HumanRef-8 WG-DASL v3.0 (13 citations) Expression BeadChip (HumanRef-8 v3) (2 citations)

6 protocols using humanref 8 v3

1

Whole-Genome Expression Analysis of hMSCs

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Whole-genome expressional analyses were carried out using Human Ref-8v3 or Human HT-12 v4 BeadArrays (Illumina, San Diego, CA). Total RNA was linearly amplified with biotin incorporation using Illumina TotalPrep kits (Life Technologies, Carlsbad, CA). The quality of the cRNA was assayed using an Agilent 2100 bioanalyzer (Santa Clara, CA). The resulting cRNA was hybrized to Illumina BeadChips (San Diego, CA), processed and read using a BeadStation array reader (Illumina, San Diego, CA) according to the manufacturer’s instructions. Numerical values of relative fluorescence units (RFU) were determined for indicated genes. Values less than 180 units were considered to be background signals. The RFU values for the two donors were averaged and these values were used to determine fold changes with respect to day 0 hMSCs. Table 1A-C and supplemental Tables present fold difference at the respective time points.
Sorrell J.M., Somoza R.A, & Caplan A.I. (2017). Human Mesenchymal Stem Cells Induced to Differentiate as Chondrocytes Follow a Biphasic Pattern of Extracellular Matrix Production. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 36(6), 1757-1766.
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2

Abdominal Adipose Tissue Transcriptome

Cited 1 time
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Global gene expression in whole abdominal subcutaneous adipose tissue (Cohort 2) and isolated AMSCs and mature adipocytes from abdominal subcutaneous adipose tissue (Cohort 3) was measured using Illumina HumanRef-8 v.3 or HumanRef-12 v.4 BeadChip microarrays, as described previously (Dankel et al., 2010 (link)). Signal intensities were quantile normalized.
Sinnott-Armstrong N., Sousa I.S., Laber S., Rendina-Ruedy E., Nitter Dankel S.E., Ferreira T., Mellgren G., Karasik D., Rivas M., Pritchard J., Guntur A.R., Cox R.D., Lindgren C.M., Hauner H., Sallari R., Rosen C.J., Hsu Y.H., Lander E.S., Kiel D.P, & Claussnitzer M. (2021). A regulatory variant at 3q21.1 confers an increased pleiotropic risk for hyperglycemia and altered bone mineral density. Cell Metabolism, 33(3), 615-628.e13.
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3

Colorectal Cancer Methylation and Expression

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Data on methylation profiling (Illumina Infinium 27k) of 12 CRC samples have been published by our group previously (Laczmanska et al. 2013 (link)).
The GSE25062 and GSE25070 datasets are part of the same experiment published by Hinoue et al. (2012 (link)). GSE25062 consists of genome-wide methylation data (Illumina Infinium 27k) for 125 colorectal tumours and 29 adjacent normal tissues. A minor part of these samples (25 colorectal tumours and matched normal adjacent colonic tissue) have also been analysed for their genome-wide expression profile (HumanRef-8 v3.0, Illumina) and the resulting data were published under accession number GSE25070.
Statistical re-analyses of the methylation and expression data were performed on samples with both types of data available.
Szmida E., Karpiński P., Leszczynski P., Sedziak T., Kielan W., Ostasiewicz P, & Sasiadek M.M. (2014). Aberrant methylation of ERBB pathway genes in sporadic colorectal cancer. Journal of Applied Genetics, 56(2), 185-192.
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4

Seasonal AR Nasal Epithelial Transcriptome

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The gene expression profile of GSE43523 was downloaded from National Center of Biotechnology Information (NCBI) Gene Expression Omnibus (GEO; http://www.ncbi.nlm.nih.gov/geo/) database. The dataset contained 7 nasal epithelial cells of seasonal AR samples and 5 nasal epithelial cells of non-allergic normal samples. The expression profile was detected based on GPL6883 Illumina HumanRef-8 v3.0 expression beadchip platform.
Song Y, & Yan Z. (2017). Exploring of the molecular mechanism of rhinitis via bioinformatics methods. Molecular Medicine Reports, 17(2), 3014-3020.
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5

Differential Expression of YTHDF1 and miR-376c in NSCLC

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The expression of YTHDF1 and miR-376c in lung cancer was obtained from the GEO
(www.ncbi.nlm.nih.gov/geo/) online database. Expression of YTHDF1
in NSCLC tissues and normal lung tissues was obtained from dataset GSE63459
based on the GPL6883 Illumina HumanRef-8 v3.0 expression beadchip platform. The
expression of miR-376c in NSCLC tissues and normal lung tissues was obtained
from the dataset GSE53882 based on the GPL18130 State Key Laboratory Human
microRNA array 1888 platform. SPSS21.0 was used to statistically analyze the
datasets to examine the differential expression of YTHDF1 and miR-376c in NSCLC
tissues and normal lung tissues.
Zhou J., Xiao D., Qiu T., Li J, & Liu Z. (2020). Loading MicroRNA-376c in Extracellular Vesicles Inhibits Properties of Non-Small Cell Lung Cancer Cells by Targeting YTHDF1. Technology in Cancer Research & Treatment, 19, 1533033820977525.
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6

Transcriptomic Profiling of 17q21.31 Haplotypes

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Microarray expression data (Illumina HumanRef-8 v3.0) were available in 120 subjects with H1/H1 or H1/H2 haplotypes at 17q21.31.
Li Y., Chen J.A., Sears R.L., Gao F., Klein E.D., Karydas A., Geschwind M.D., Rosen H.J., Boxer A.L., Guo W., Pellegrini M., Horvath S., Miller B.L., Geschwind D.H, & Coppola G. (2014). An Epigenetic Signature in Peripheral Blood Associated with the Haplotype on 17q21.31, a Risk Factor for Neurodegenerative Tauopathy. PLoS Genetics, 10(3), e1004211.
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