Diamine oxidase (from porcine kidney, ≥0.05 unit·mg−1 solid, EC 1.4.3.6, DAO), histamine, chitosan, acetic acid, phosphate buffer solutions, potassium chloride, sulphuric acid, potassium tetrachloroplatinate(II), hydrogen peroxide (H2O2), l -lysine (Lys), l -tyrosine (Tyr), l -histidine (His), l -tryptophan (Trp), putrescine (1,4-diaminobutane), cadaverine (1,5-diaminopentane), and tyramine (2-(p-Hydroxyphenyl)ethylamine) were all acquired from Sigma-Aldrich (St. Louis, MO, USA). The Veratox® kit ELISA, dedicated to histamine quantification, was purchased from Neogen® Corporation (Lansing, MI, USA). All aqueous solutions required in the electrochemical studies were freshly prepared from the solid and pure compounds with ultrapure water (resistivity 18.3 MΩ·cm) obtained in the lab with professional equipment (Milli-Q Simplicity® Water Purification System from Millipore Corporation, Billerica, MA, USA).
Diamine oxidase
Manufactured by Merck Group
Sourced in United States
Diamine oxidase is an enzyme that catalyzes the oxidative deamination of diamines, such as histamine and putrescine. It plays a role in the metabolism and regulation of these compounds in the body.
Automatically generated - may contain errors
Lab products found in correlation
Chitosan, by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Sulphuric acid, by Merck Group (1 mentions)
Histamine, by Merck Group (1 mentions)
Milli q simplicity water purification system, by Merck Group (1 mentions)
Tyramine, by Merck Group (1 mentions)
Cadaverine, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Citrate synthase, by Merck Group (1 mentions)
Trypsin, by AppliChem (1 mentions)
N1 acetylspermidine, by Cayman Chemical (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
3 protocols using diamine oxidase
1
Electrochemical Determination of Histamine
2
Comprehensive Analytical Protocols for Polyamine Modifications
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All basic chemicals were purchased at the highest available purity level from Sigma-Aldrich, Merck, ROTH, Acros Organics or Applichem. The substrates, enzymes and inhibitors used in the assays were obtained from the following suppliers: N-(5-aminopentyl) acetamide (acetylcadaverine) and N-(4-aminobutyl) acetamide (acetylputrescine) from TCI, N1-Acetylspermine from FLUKA, N1-Acetylspermidine and SAHA from Cayman Chemical Company,
Boc-K-(Tfa)-AMC and Boc-K-(Ac)-AMC from Bachem, citrate synthase, diamine oxidase and horseradish peroxidase type 1 from Sigma-Aldrich, trypsin from Applichem and the malatdehydrogenase from Calbiochem. SATFMK was synthesized according to [39 (link)]. PA0321, PA1409 and PA3774 were recombinantly produced as described before [30 (link)]. The acetyl-CoA synthetase was recombinantly expressed and purified according to Fierke et al. [31 (link)] using a plasmid that was kindly provided by the cited working group.
Boc-K-(Tfa)-AMC and Boc-K-(Ac)-AMC from Bachem, citrate synthase, diamine oxidase and horseradish peroxidase type 1 from Sigma-Aldrich, trypsin from Applichem and the malatdehydrogenase from Calbiochem. SATFMK was synthesized according to [39 (link)]. PA0321, PA1409 and PA3774 were recombinantly produced as described before [30 (link)]. The acetyl-CoA synthetase was recombinantly expressed and purified according to Fierke et al. [31 (link)] using a plasmid that was kindly provided by the cited working group.
3
Electrochemical Biosensor for Biogenic Amines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Diamine oxidase (DOX, 0.11 U mg -1 ), potassium hexacyanoferrate (III), biogenic amines (histamine, putrescine, spermine, tyramine, tryptamine, and phenylethylamine), and glutaraldehyde (GA) were supplied by Sigma-Aldrich. The fresh tuna and mackerel were purchased in a local market.
Working solutions of DOX, GA, histamine, and the other biogenic amines were prepared in 0.1 M phosphate buffer (PB) pH 7.2. This pH was chosen because the supplier indicated that this is the optimum pH to dissolve the enzyme and because the ideal pH range is 6.3-7.4 when histamine is used as the enzymatic substrate.
Type I deionized water (resistivity = 18.2 MΩ cm) was used throughout the work. All the chemicals were of analytical reagent grade and were used without further treatment or purification.
A Metrohm-Autolab potentiostat/galvanostat (PGSTAT 101) controlled by NOVA software (v1.10) was used for the electrochemical measurements. Screen-printed electrodes (printed on a ceramic substrate -3.4 cm × 1.0 cm), consisting of a circular-shaped carbon-ink working electrode (WE, d = 4 mm), a silver-ink pseudoreference electrode (RE), and a carbon-ink counter electrode (CE) were used. These screen-printed carbon electrodes (SPCEs) and the suitable connector were purchased from DropSens.
Working solutions of DOX, GA, histamine, and the other biogenic amines were prepared in 0.1 M phosphate buffer (PB) pH 7.2. This pH was chosen because the supplier indicated that this is the optimum pH to dissolve the enzyme and because the ideal pH range is 6.3-7.4 when histamine is used as the enzymatic substrate.
Type I deionized water (resistivity = 18.2 MΩ cm) was used throughout the work. All the chemicals were of analytical reagent grade and were used without further treatment or purification.
A Metrohm-Autolab potentiostat/galvanostat (PGSTAT 101) controlled by NOVA software (v1.10) was used for the electrochemical measurements. Screen-printed electrodes (printed on a ceramic substrate -3.4 cm × 1.0 cm), consisting of a circular-shaped carbon-ink working electrode (WE, d = 4 mm), a silver-ink pseudoreference electrode (RE), and a carbon-ink counter electrode (CE) were used. These screen-printed carbon electrodes (SPCEs) and the suitable connector were purchased from DropSens.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!