Log In Sign up
The largest database of trusted experimental protocols

Evom endohm

Manufactured by World Precision Instruments
Visit Supplier
Sourced in United States

The EVOM/EndOhm is a laboratory instrument designed to measure the electrical resistance and impedance of cell monolayers and other biological samples. It is a versatile tool used in various fields of research, including cell biology, tissue engineering, and pharmaceutical development.

Automatically generated - may contain errors

Lab products found in correlation

Synergy htx multi mode plate reader, by Agilent Technologies (2 mentions)

Close spelling variants of this product

EVOM EndOhm-12chamber ENDOHM-6 EVOM electrode EndOhm

6 protocols using evom endohm

1

Transwell Permeability Assay for HT29-MTX

Check if the same lab product or an alternative is used in the 5 most similar protocols
HT29-MTX E12 cells were grown on 0.4 μm pore Transwell® filter systems and monolayers were monitored for electrical resistance using an epithelial volt-ohmmeter (EVOM/EndOhm; World Precision Instruments, USA). Molecule flux assays utilized 25 μg 4 kDa FITC Dextran in the top chamber. This amount was delivered in 250 μL and 10 μL of PBS 2% FBS in LI and ALI conditions, respectively. Contents of the bottom chamber were assessed after 1 hr. 4 kDa FITC Dextran detection was performed using a BioTek Synergy HTX Multi-Mode plate reader (Agilent, USA).
Johnson B., Panek P., Yu A., Fischer E., Koba M., Hermosillo D.M, & Capaldo C.T. (2022). Interferon gamma upregulates the cytokine receptors IFNGR1 and TNFRSF1A in HT-29-MTX E12 cells. Cytokine, 156, 155892.
+ Open protocol
+ Expand
2

Epithelial Cell Electrical Resistance

Check if the same lab product or an alternative is used in the 5 most similar protocols
IECs were grown on permeable supports, and monolayers were monitored for electrical resistance using an epithelial volt-ohmmeter (EVOM/EndOhm; World Precision Instruments, Sarasota, FL).
Capaldo C.T., Farkas A.E., Hilgarth R.S., Krug S.M., Wolf M.F., Benedik J.K., Fromm M., Koval M., Parkos C, & Nusrat A. (2014). Proinflammatory cytokine-induced tight junction remodeling through dynamic self-assembly of claudins. Molecular Biology of the Cell, 25(18), 2710-2719.
+ Open protocol
+ Expand
3

Transwell Permeability Assay for HT29-MTX

Check if the same lab product or an alternative is used in the 5 most similar protocols
HT29-MTX E12 cells were grown on 0.4 μm pore Transwell® filter systems and monolayers were monitored for electrical resistance using an epithelial volt-ohmmeter (EVOM/EndOhm; World Precision Instruments, USA). Molecule flux assays utilized 25 μg 4 kDa FITC Dextran in the top chamber. This amount was delivered in 250 μL and 10 μL of PBS 2% FBS in LI and ALI conditions, respectively. Contents of the bottom chamber were assessed after 1 hr. 4 kDa FITC Dextran detection was performed using a BioTek Synergy HTX Multi-Mode plate reader (Agilent, USA).
Johnson B., Panek P., Yu A., Fischer E., Koba M., Hermosillo D.M, & Capaldo C.T. (2022). Interferon gamma upregulates the cytokine receptors IFNGR1 and TNFRSF1A in HT-29-MTX E12 cells. Cytokine, 156, 155892.
+ Open protocol
+ Expand
4

Evaluating Epithelial Integrity via TER

Check if the same lab product or an alternative is used in the 5 most similar protocols
Epithelial integrity of ALI cultures was evaluated by transepithelial electrical resistance (TER) measurements using an EVOM/Endohm (World Precision Instruments, Sarasota, Fla) as described in the Methods section of this article's Online Repository. Wells not building up sufficiently (TER < 200 V 3 cm 2 ) were not included in experiments.
Steelant B., Seys S.F., Van Gerven L., Van Woensel M., Farré R., Wawrzyniak P., Kortekaas Krohn I., Bullens D.M., Talavera K., Raap U., Boon L., Akdis C.A., Boeckxstaens G., Ceuppens J.L, & Hellings P.W. (2018). Histamine and T helper cytokine-driven epithelial barrier dysfunction in allergic rhinitis. The Journal of allergy and clinical immunology, 141(3).
+ Open protocol
+ Expand
5

Monolayer Intestinal Integrity Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monolayer intestinal integrity and permeability were assessed with a dual planar electrode instrument (Endohm Evom; World Precision Instruments, Sarasota, FL) following the manufacturer’s instructions. TEER values were monitored every day for a total of 10–11 days during monolayer development. Data are expressed as resistance per square centimeter (Ω/cm2).
Senger S., Ingano L., Freire R., Anselmo A., Zhu W., Sadreyev R., Walker W.A, & Fasano A. (2018). Human Fetal-Derived Enterospheres Provide Insights on Intestinal Development and a Novel Model to Study Necrotizing Enterocolitis (NEC). Cellular and Molecular Gastroenterology and Hepatology, 5(4), 549-568.
+ Open protocol
+ Expand
6

Intestinal Permeability Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Intestinal permeability was assessed ex vivo by measuring transepithelial electrical resistance (TEER) of biopsy explants using a dual planar electrode (Endohm Evom; World Precision Instruments, Sarasota, FL), expressed in Ω cm2 and normalized by the baseline resistance values. After their collection, four small-intestine biopsies were oriented on presterilized filter paper with the villi facing upward and mounted onto the modified microsnapwell system as previously described by El Asmar et al. [19 (link)]. After 30 min incubation at 37 °C, a baseline TEER measurement (time 0) was obtained. Subsequently, a pair of biopsy explants were then luminally exposed to Dulbecco’s Modified Eagle’s Medium (DMEM) alone and a pair of biopsy explants were exposed to pepsin-trypsin digested gliadin (PT-gliadin), prepared as previously described [20 (link)] and suspended in DMEM at a final concentration of 1 mg/mL. TEER values were then monitored at 30 min intervals for a total of 120 min. TEER values for each pair of biopsies were averaged and data was expressed as the fractional change from the baseline TEER.
Hollon J., Leonard Puppa E., Greenwald B., Goldberg E., Guerrerio A, & Fasano A. (2015). Effect of Gliadin on Permeability of Intestinal Biopsy Explants from Celiac Disease Patients and Patients with Non-Celiac Gluten Sensitivity. Nutrients, 7(3), 1565-1576.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!