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30 protocols using nickel chloride hexahydrate

1

Synthesis of Cobalt-Nickel Materials

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Several chemicals have been purchased from Sigma Aldrich Karachi Pakistan, including cobalt chloride hexahydrate (98%), nickel chloride hexahydrate (99.9%), urea (99%), ethanol (99%), potassium dihydrogen phosphate (98%), disodium hydrogen phosphate (98%), sodium chloride (99.5%), and sodium hydroxide (97%). All chemicals received are of high analytical quality and can be applied without further treatment. A deionized water solution was used to prepare growth precursors for the synthesis of the materials.
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2

Synthesis and Characterization of Conductive Polymers

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All reagents were used without further purifications. Ferric chloride hexahydrate (FeCl3·6H2O, 97%), nickel chloride hexahydrate (NiCl2·6H2O), ammonium persulfate ((NH4)2S2O8), aniline (C6H5NH2) and o-toluidine (C7H9N) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium hydroxide (NaOH) and hydrochloric acid (HCl) were obtained from Merck (Germany). 2,4-Dichlorophenol (2,4-DCP, C6H4Cl2O) was obtained from PubChem (St. Rockville Pike, Bethesda, USA). All chemicals were of analytical grade and used as received. Ultrapure water was used for the preparation of all solutions. Stock solutions and all dilute test solutions of 2,4-DCP were stored in brown bottles and kept in darkness to prevent degradation.
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3

Membrane Fabrication and Alloy Synthesis

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The polymers used for membrane preparation included cellulose acetate (average Mn ~50,000 by GPC), obtained from Aldrich (Munich, Germany), and poly(methylemethacrylate), purchased from Lanxess (Liyang, China). The solvent mixture used included 1,4-dioxan (99.5%), purchased from Adwic, El-Nasr Pharmaceutical Chemicals Company (Qalyubia, Egypt); acetone (99%), obtained from El-salam for Chemical Industries (Giza, Egypt); methanol (99.8%, HPLC grade), obtained from Fisher (Loughborough, UK); and acetic acid (99.8% for biochemistry), purchased from Acros Organics (New Jersey, USA). Diethyle phthalate (99%) was obtained from Loba Chemie (Mumbai, India). Lithium chloride anhydrous was purchased from Fisher (Loughborough, UK). The chemicals used for alloy synthesis included nickel chloride hexahydrate (NiCl2·6H2O, 98%) and ferrous chloride tetrahydrate (FeCl2·4H2O, 99.99%) as sources of metal ion, which were purchased from Sigma (Darmstadt, Germany). Hydrazine hydrate (N2H4·H2O, 99%) was obtained from Fisher (Horsham, UK) as a reducing agent. Sodium hydroxide (NaOH, 98%) catalyst was purchased from Trading Dynamic Co. (TDC, Cairo, Egypt). Distilled water was used as a solvent.
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4

Trace Metal Analysis Protocol

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3-Nitro-L-tyrosine, L-tyrosine (≥98%), mercury nitrate monohydrate (≥98.5%), lead nitrate (≥99%), cadmium acetate dihydrate (98%), copper chloride (≥99%), calcium chloride (≥93%), magnesium sulfate (≥99.5%), zinc chloride (≥98%), nickel chloride hexahydrate (≥98%), manganese chloride tetrahydrate (≥98%), iron(III) chloride (97%), chromium chloride hexahydrate (96%), sodium (meta)arsenite (≥90%), and tetrabutylammonium perchlorate (TBAP, ≥99%) were purchased from Sigma-Aldrich (Milwaukee, WI, USA). Ultrapure water (UPW) with a resistivity of 18.2 MΩ·cm was obtained from a PURELAB Option-Q water-purification system (High Wycombe, Buckinghamshire, UK).
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5

Electrochemical Sensor Fabrication and Characterization

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Caffeic acid, protoporphyrin IX (PPIX), chitosan, N, N-dicyclohexyl carbodiimide (DCC), bicarbonate, D-glucose, uric acid, L-ascorbic acid, acetaminophen, ferric chloride, cobaltous chloride, nickel chloride hexahydrate, cupric chloride, zinc chloride, and manganese (II) chloride tetrahydrate were purchased from Sigma-Aldrich (Saint Louis, MO, USA). N, N-dimethylformamide (DMF), and MWCNTs (30–50 nm diameter, 2 μm average length and 95% purity) were purchased from Aladdin. Acetic acid, KCl, ethanol, K3Fe[(CN)6], Na2HPO4, and citric acid were purchased from Tianjin Damao Chemical Reagent Factory (Tianjin, China). All the chemicals and reagents were of analytical-reagent grade and used as received without further purification. Deionized water was used throughout the experiments unless otherwise indicated.
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6

Green Synthesis of Functionalized Nanoparticles

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Hydrazine monohydrate (N2H4·H2O) (98%), nickel chloride hexahydrate (NiCl2·6H2O) (99.99%), methylene blue (95%), tri-ethylene glycol (TEG) (99%) and dopamine hydrochloride (98%) were purchased from Sigma-Aldrich. Sodium borohydride (98%) was obtained from Daejung Chemical & Metals. 4-Nitrophenol (98%) and sodium hydroxide (98%) were purchased from Loba Chemie, chloroauric acid (>47.8% gold basis) was bought from Shanghai Bojing Chemical. The chemicals and reagents were analytical grade and used without further purification. Milli-Q water was used as a solvent for preparing the solutions unless otherwise notified.
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7

Metal-Doped Titanium Dioxide Synthesis

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The reagents used in this work are the following: Titanium dioxide (Degussa P25, 75% anatase, 25% rutile); Nickel chloride hexahydrate (reagent grade, Sigma-Aldrich); Cobalt nitrate hexahydrate (reagent grade, Sigma-Aldrich); Hydrogen tetrachloroaurate trihydrate (reagent grade, Sigma-Aldrich); Chloroplatinic acid hydrate (reagent grade, Sigma-Aldrich); Silver nitrate (reagent grade, Sigma-Aldrich); Palladium nitrate dehydrate (reagent grade, Sigma-Aldrich); Methanol (anhydrous, ≥ 99.8% (HPLC), Sigma-Aldrich); Ammonia gas (anhydrous, BOC); Argon gas (99.99%, BOC); Helium gas (99.99%, BOC); Nitrogen gas (99.99%, BOC).
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8

Heavy Metal Cell Culture Protocol

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Leibovitz's (L-15) culture medium, Penicillin Streptomycin (PEST), Gentamicin and Trypsin-EDTA (0.5%) were purchased from Gibco, (Life Technologies, USA). Fetal Bovine Serum (FBS), Hydrocortisone, Arsenic (III) oxide (As2O3), Cadmium Sulfate Hydrate (3CdSO4.8H2O), Copper (II) Sulfate pentahydrate (CuSO4.5H2O), Nickel Chloride hexahydrate (NiCl2.6H2O), and Lead (II) Nitrate [Pb(NO3)2] were purchased from Sigma-Aldrich (USA). Phosphate Saline Buffer (PBS, Dulbecco A) was purchased from Oxoid Ltd (England). Instant Ocean Sea salt was purchased from Aquarium Systems (France).
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9

Synthesis of Multifunctional Nanostructures

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For the preparation of ZnO NPs, S-g-C3N4 NS, NZO NPs, and NZO-x-SCN NC, chemicals of analytical grade were used. Nickel chloride hexahydrate (NiCl2·6H2O), zinc nitrate (Zn(NO3)2·6H2O), ammonium hydroxide (NH4OH), benzoquinone (C6H4O2), thiourea ((NH2)2CS), ammonium oxalate (C2H8N2O4), isopropanol (C3H8O), methylene blue (C16H18N3SCl), sodium lauryl sulfate, and absolute ethanol (C2H5OH), were purchased from Sigma-Aldrich.
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10

Synthesis of Cobalt and Nickel Compounds

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Cobalt chloride hexahydrate (CoCl2·6H2O), nickel chloride hexahydrate (NiCl2·6H2O), urea (CH4N2O), were purchased from Sigma-Aldrich (Stockholm, Sweden) and used without any further purification. All chemicals were of analytical grade reagents and were used as received.
All glassware was washed by soaking in 3 M HNO3 overnight followed by washing with detergent water. It was then thoroughly washed with tap water and finally rinsed at least 3 times with doubly distilled water. The glassware was then dried in an oven at 110 °C.
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