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Anti human cd31

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Anti-human CD31 is a laboratory reagent used for the detection and analysis of CD31, also known as PECAM-1, a cell surface protein expressed on endothelial cells, platelets, and certain immune cells. This reagent can be utilized in various immunoassay techniques to identify and quantify CD31-positive cells.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Lsm 710, by Zeiss (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Rabbit anti vwf, by Agilent Technologies (1 mentions) Sheep anti vwf, by Abcam (1 mentions) Rabbit anti eea1, by Cell Signaling Technology (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Af385, by R&D Systems (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Dmire2 inverted microscope camera, by Leica Microsystems (1 mentions) Tcs sp2 acousto optical beam splitter confocal system, by Leica Microsystems (1 mentions) Purified class and species matched iggs, by Vector Laboratories (1 mentions) Rm 9107 s, by Agilent Technologies (1 mentions) Anti human ido1, by Cell Signaling Technology (1 mentions) Anti human cd8, by Agilent Technologies (1 mentions) Anti human cd3, by Thermo Fisher Scientific (1 mentions) Ultraview universal dab detection kit, by Roche (1 mentions) Benchmark ultra, by Roche (1 mentions)

6 protocols using anti human cd31

1

Immunohistochemical Profiling of Cells

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Antibodies used include: sheep anti-FVIII (Affinity Biologicals), rabbit anti-VWF (Dako), sheep anti-VWF (Abcam, Cambridge, UK), rabbit anti-EEA1 (Cell Signalling Technology, Beverly, MA, USA), rabbit anti-SCARA5 (HPA024661, Sigma Aldrich), mouse anti-SCARA5 (αh-SR5.2), anti-human CD31 (Dako), anti-human CD68 (Santa Cruz Biotechnology, Dallas, USA), anti-human synaptopodin (R&D, Minneapolis, USA), mouse anti-human CD34 (QBEnd-10, ThermoFisher Scientific, Waltham, USA) and mouse anti-human CD8α (4B11, ThermoFisher Scientific).
Swystun L.L., Ogiwara K., Lai J.D., Ojala J.R., Rawley O., Lassalle F., Notley C., Rengby O., Michels A., Nesbitt K., Tryggvason K, & Lillicrap D. (2019). The scavenger receptor SCARA5 is an endocytic receptor for von Willebrand factor expressed by littoral cells in the human spleen. Journal of thrombosis and haemostasis : JTH, 17(8), 1384-1396.
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2

Visualizing iCMs and iECs in Scaffolds

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Immunofluorescence staining was performed after cell seeding to visualize the in vitro organization of human iCMs and iECs within the scaffolds, based on phenotypic markers of troponin-T (TNNT) for iCMs and CD31 for iECs. The cell-seeded scaffolds were fixed in 4% paraformaldehyde (Electron Microscopy Sciences), permeabilized in 0.1% Triton X-100 (Sigma-Aldrich), and then blocked with 1% bovine serum albumin (Wanjare et al., 2017 (link)). The scaffolds were then incubated with primary antibodies directed against anti-human troponin-T (Sigma) and anti-human CD31 (Dako), followed by secondary antibodies conjugated to Alexa Fluor 488 or Alexa Fluor 594 (both from Thermo Fisher Scientific). The samples were then washed in PBS, and total nuclei were counterstained using Hoechst 33342 (Thermo Fisher Scientific) nuclear dye. Samples were stored at 4°C in the dark and imaged using a laser scanning confocal microscope (LSM710, Zeiss).
Wanjare M., Kawamura M., Hu C., Alcazar C., Wang H., Woo Y.J, & Huang N.F. (2019). Vascularization of Engineered Spatially Patterned Myocardial Tissue Derived From Human Pluripotent Stem Cells in vivo. Frontiers in Bioengineering and Biotechnology, 7, 208.
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3

Immunohistochemical Staining of Tissue Sections

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Formalin-fixed, paraffin-embedded tissue sections (5μm) were deparaffinized and either directly stained with hematoxylin and eosin (H&E) or immersed in an antigen retrieval solution (Citrate-EDTA buffer: 10mM Citric Acid, 2mM EDTA, 0.05% Tween-20, pH 6.2;) for 20 minutes. Frozen sections (10μm) fixed in acetone at −20°C for 10 minutes and evaporated at room temperature for 20 minutes. Sections were blocked for 30 minutes in 5% serum and incubated overnight with anti-human VE-Cadherin (1:40, C19, Sigma), anti-human PDGFRβ (1:40, AF385, R&D Systems), or anti–human CD31 (1:40, clone JC70A, Dako). Purified class- and species-matched IgGs (Vector Lab) were used as controls. Sections were incubated with fluorescein or Texas Red conjugated secondary antibody (Vector Laboratories). Slides were stained with the nuclear marker DAPI (Molecular Probe, Eugene, OR). Images were acquired using a Leica TCS sp2 Acousto-Optical Beam Splitter confocal system equipped with DMIRE2 inverted microscope camera (Leica Microsystems, Wetzlar, Germany).
Couto J.A., Huang L., Vivero M.P., Kamitaki N., Maclellan R.A., Mulliken J.B., Bischoff J., Warman M.L, & Greene A.K. (2016). Endothelial Cells from Capillary Malformations are Enriched for Somatic GNAQ Mutations. Plastic and reconstructive surgery, 137(1), 77e-82e.
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4

Immunohistochemical Profiling of Glioma

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Formalin-fixed, paraffin-embedded WHO grade 3 to 4 primary and recurrent human glioma tissues were obtained from the archives of the Department of Neuropathology, Heidelberg and were cut to 3 μm sections and processed using a Ventana Benchmark Ultra immunostainer. The DAB staining procedure included blocking of endogenous peroxidase with 0.3% H2O2 for 3 minutes and treatment with blocking buffer, followed by incubation with the primary antibody at 4°C overnight. Incubation was followed by ABC-Kit-Solution for 30 minutes, washing and counter-staining with hematoxylin for 2 minutes. Positivity was visualized with 3′3 diaminobenzidine (DAB; brown) using the ultraView Universal DAB Detection Kit (Ventana Medical Systems, Inc.). The following primary antibodies were used: anti-human CD8 (1:50, Dako M7103), anti-human CD3 (1:200, Thermo Scientific #RM-9107-S), anti-human CD31 (1:10, Dako M0823), and anti-human IDO1 (1:200, Cell Signaling Technology). Density of IDO1+ cells was evaluated semiquantitatively by overall impression at low microscopic magnification (100×) within 1 mm2 and if any specific positive staining was identified it was judged present.
Abu Hejleh A., Huck K., Jähne K., Tan C., Lanz T., Epping L., Sonner J., Meuth S., Henneberg A., Opitz C., Herold-Mende C., Sahm F., Platten M, & Sahm K. (2023). Endothelial Indoleamine-2,3-Dioxygenase-1 is not Critically Involved in Regulating Antitumor Immunity in the Central Nervous System. International Journal of Tryptophan Research : IJTR, 16, 11786469231153111.
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5

Histological and Immunostaining Analysis of Vascular Grafts

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For standard histology and immunohistochemistry, vascular grafts were explanted, fixed in 4% paraformaldehyde, and embedded in paraffin. Paraffin blocks were cut into 5 µm sections and stained for hematoxylin-eosin, Masson's trichrome, and orcein to study morphology, connective tissue, and elastic tissue, respectively. The following primary antibodies were used for immunostaining: anti-Ku80 (EPR9111(B), Abcam), anti-CD31 (557703, BDBiosciences), anti-human CD31 (DAKO, clone JC70A), anti-smooth muscle actin (Abcam), and Hoechst. Alexa Fluor 488 and 568 (ThermoFisher Scientific) conjugated secondary antibodies were used. DAPI (4',6-Diamidino-2-phenylindole) nucleus staining was also used. Images were captured in Zeiss Axio Imager 2 or Nikon microscope (Eclipse 80i) connected to a camera (DS-Ri1; Nikon, Tokyo, Japan) and Elements software.
Gara E., Zucchelli E., Nemes A., Jakus Z., Ajtay K., Kemecsei É., Kiszler G., Hegedűs N., Szigeti K., Földes I., Árvai K., Kósa J., Kolev K., Komorowicz E., Padmanabhan P., Maurovich-Horvat P., Dósa E., Várady G., Pólos M., Hartyánszky I., Harding S.E., Merkely B., Máthé D., Szabó G., Radovits T, & Földes G. (2022). 3D culturing of human pluripotent stem cells-derived endothelial cells for vascular regeneration. Theranostics, 12(10), 4684-4702.
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6

Histological and Immunofluorescence Analysis

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Punch biopsy specimens (3-mm diameter) were processed for histology and immunofluorescence as described previously. 24 Antibodies included anti-human K1 (Novus Biologicals, Littleton, Colo.), anti-human Lam332 (Santa Cruz, Nunningen, Switzerland), anti-human Tropoelastin (Elastin Products Co., Owensville, Mo.), anti-human CD31 (Dako, Switzerland), and antihuman Keratin19 (Dako, Switzerland).
Meuli M., Hartmann-Fritsch F., Hüging M., Marino D., Saglini M., Hynes S., Neuhaus K., Manuel E., Middelkoop E., Reichmann E, & Schiestl C. (2019). A Cultured Autologous Dermo-epidermal Skin Substitute for Full-Thickness Skin Defects: A Phase I, Open, Prospective Clinical Trial in Children. Plastic and reconstructive surgery, 144(1).
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