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Alexa 568 goat anti rabbit

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Alexa Fluor 568 goat anti-rabbit is a fluorescently-labeled secondary antibody used for detection and visualization in various immunoassay and imaging applications. It is designed to specifically bind and detect rabbit primary antibodies.

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Lab products found in correlation

Goat anti mouse alexa 488, by Thermo Fisher Scientific (11 mentions) Goat anti rabbit alexa 488, by Thermo Fisher Scientific (5 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (4 mentions) Lsm 700, by Zeiss (4 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions) Deltavision omx blaze, by GE Healthcare (2 mentions) Slowfade gold, by Thermo Fisher Scientific (2 mentions) Rabbit anti tom20, by Santa Cruz Biotechnology (2 mentions) Donkey anti sheep alexa 488, by Thermo Fisher Scientific (2 mentions) Santa cruz biotech sc 11415, by Santa Cruz Biotechnology (2 mentions) Goat anti mouse alexa 568, by Thermo Fisher Scientific (2 mentions) Goat anti rat alexa488, by Thermo Fisher Scientific (2 mentions) Rabbit anti mcherry, by Abcam (2 mentions) Goat anti guinea pig alexa 647, by Thermo Fisher Scientific (2 mentions) Mouse anti flag, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Mouse anti engrailed, by Developmental Studies Hybridoma Bank (2 mentions) Hoechst, by Thermo Fisher Scientific (2 mentions) Las x software, by Leica (2 mentions) Vectashield hardset antifade mounting medium, by Vector Laboratories (2 mentions)

Close spelling variants of this product

Alexa 568 (337 citations) Goat anti-rabbit IgG Alexa 568 (29 citations) Anti-rabbit ALEXA 568 (23 citations) Alexa Fluor 568 goat anti-rabbit antibody (23 citations) Alexa 568-conjugated goat anti-rabbit IgG (16 citations) Alexa 568-conjugated goat anti-rabbit (14 citations) Alexa Fluor 568-conjugated goat anti-rabbit secondary antibody (11 citations) Alexa Fluor 568-labeled goat anti-rabbit IgG (11 citations) Alexa fluor® 568 goat anti-rabbit IgG secondary antibody (6 citations) Alexa 488 goat anti-Rabbit/Alexa 568 goat anti-mouse (5 citations)

39 protocols using alexa 568 goat anti rabbit

1

Selective Permeabilization of Cell Membranes

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HEK293 cells grown on glass coverslips were fixed with 3% paraformaldehyde in PBS for 10 min. and washed in PBS. For selective permeabilization of the plasma membrane, cells were incubated in buffer S (10 mM HEPES-KOH, pH 7.4; 0.3 M sucrose; 0.1 M KCl; 2.5 mM MgCl2; 1 mM EDTA, and 50 μg/ml digitonin (Sigma)) for 3 min on ice [24 (link)]. For permeabilization of all membranes, cells were incubated with 1% Triton X-100 in PBS for 10 min and blocked with 1% BSA in PBS. Mouse anti-FLAG M2 antibody (1:500, Sigma, #A8592) and rabbit anti-GFP antibody (1:200, Life Technologies, #A11122) were diluted in blocking solution and incubated for 1 hr. at room temperature. Primary antibody binding was visualized using fluorescent dye-conjugated secondary antibodies: goat anti mouse Alexa 568 (Life Technologies, #A11032) or goat anti rabbit Alexa 568 (Life Technologies, #A11036) incubated for 45 min. DAPI (Life Technologies) staining was used to visualize nuclei. The samples were mounted with ProLong Gold antifade reagent (Life Technologies) and visualized using inverted fluorescence microscopy (Leica DMI6000) or a Leica SP5 (II) laser scanning confocal microscope (Leica, Buffalo Grove, IL) equipped with 40X (1.30 NA) and 63X (1.4–0.6 NA) oil immersion lenses. Leica LAS AF Lite software was used for recording and image processing.
Witwicka H., Hwang S.Y., Reyes-Gutierrez P., Jia H., Odgren P.E., Donahue L.R., Birnbaum M.J, & Odgren P.R. (2015). Studies of OC-STAMP in Osteoclast Fusion: A New Knockout Mouse Model, Rescue of Cell Fusion, and Transmembrane Topology. PLoS ONE, 10(6), e0128275.
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2

Microtubule Visualization in HeLa Cells

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HeLa cells were fixed with 4% paraformaldehyde for 10 min, washed 3 times for 5 minutes with PBS, and permeabilized with 0.1% Triton-X for 15 min. Microtubules in fixed HeLa were stained with primary antibodies (Sheep Anti-Tubulin, Cytoskeleton ATN02) in blocking buffer 1× PBS with 0.1% Triton X-100 and 2% normal donkey serum (PBT) at a concentration of 10 μg/mL for 1–4 hours and then washed in PBS three times for 5 minutes each. Specimens were then incubated with secondary antibodies (Donkey Anti-Sheep Alexa 488, Life Technologies, 10 μg/mL) in PBT for 1–4 hours and then washed in PBS three times for 5 minutes. 50 μm brain tissue slices were prepared and stained with primary and secondary antibodies (Rabbit Anti-Tom20, Santa Cruz Biotech sc-11415 and Goat Anti-Rabbit Alexa 568 (Life Technologies)) as described below. Super-resolution structured illumination microscope imaging was performed on a Deltavision OMX Blaze (GE healthcare) SIM microscope with 100× 1.40 NA (Olympus) oil objective. Stained cells were imaged with SlowFade Gold (Invitrogen) antifade reagent for suppression of photobleaching and refractive index matching for pre-expansion imaging.
Tillberg P.W., Chen F., Piatkevich K.D., Zhao Y., Yu C.C., English B.P., Gao L., Martorell A., Suk H.J., Yoshida F., DeGennaro E.M., Roossien D.H., Gong G., Seneviratne U., Tannenbaum S.R., Desimone R., Cai D, & Boyden E.S. (2016). Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nature biotechnology, 34(9), 987-992.
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3

Immunofluorescence Staining of β-tubulin-III and YFP

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Immunofluorescence was performed as described previously (Kang et al., 2013 (link)), using mouse monoclonal antibodies against β-tubulin-III (R and D Systems MAB1195) and rabbit polyclonal antibodies against YFP (Life Technologies A6455). Secondary antibodies used were goat anti-rabbit-Alexa568 (Life Technologies A-11011) and goat anti-mouse-Alexa488 (Life Technologies A-11001).
Shen Z., Kang J., Shakya A., Tabaka M., Jarboe E.A., Regev A, & Tantin D. (2017). Enforcement of developmental lineage specificity by transcription factor Oct1. eLife, 6, e20937.
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4

Immunohistochemistry of DLB Midbrain

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Immunohistochemistry of midbrain sections of a patient with DLB was performed as described previously [62 (link)]. In brief, 5 μm thick paraffin-embed human midbrain sections were de-paraffinized by xylol and a descending series of alcohols and subjected to antigen retrieval by steaming in dH2O for 30 min. Sections were blocked with 5 % goat serum in PBS-T for 1 h and incubated with primary antibodies mouse-anti- α-synuclein( 1:100, 4B12, Covance, Princeton, USA) and rabbit-anti-SOD1 (1:70, [60 (link)]) in 2,5 % goat serum at 4 °C overnight. After washing with PBS, sections were incubated with secondary antibodies (1:500, goat-anti-rabbit-Alexa568 and goat-anti-mouse-Alexa-488, both Life technology, Carlsbad, USA) in 1 % goat-serum for 1 h at RT. Subsequently sections were washed with PBS, blocked with 1 % Sudan Black (Sigma, St.Louis, USA) for 2 min and coverslipped with Fluoromont®G (SouthernBioTech, Birmingham, USA). As control, sections were stained without primary antibodies.
Helferich A.M., Ruf W.P., Grozdanov V., Freischmidt A., Feiler M.S., Zondler L., Ludolph A.C., McLean P.J., Weishaupt J.H, & Danzer K.M. (2015). α-synuclein interacts with SOD1 and promotes its oligomerization. Molecular Neurodegeneration, 10, 66.
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5

Microtubule Visualization in HeLa Cells

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HeLa cells were fixed with 4% paraformaldehyde for 10 min, washed 3 times for 5 minutes with PBS, and permeabilized with 0.1% Triton-X for 15 min. Microtubules in fixed HeLa were stained with primary antibodies (Sheep Anti-Tubulin, Cytoskeleton ATN02) in blocking buffer 1× PBS with 0.1% Triton X-100 and 2% normal donkey serum (PBT) at a concentration of 10 μg/mL for 1–4 hours and then washed in PBS three times for 5 minutes each. Specimens were then incubated with secondary antibodies (Donkey Anti-Sheep Alexa 488, Life Technologies, 10 μg/mL) in PBT for 1–4 hours and then washed in PBS three times for 5 minutes. 50 μm brain tissue slices were prepared and stained with primary and secondary antibodies (Rabbit Anti-Tom20, Santa Cruz Biotech sc-11415 and Goat Anti-Rabbit Alexa 568 (Life Technologies)) as described below. Super-resolution structured illumination microscope imaging was performed on a Deltavision OMX Blaze (GE healthcare) SIM microscope with 100× 1.40 NA (Olympus) oil objective. Stained cells were imaged with SlowFade Gold (Invitrogen) antifade reagent for suppression of photobleaching and refractive index matching for pre-expansion imaging.
Tillberg P.W., Chen F., Piatkevich K.D., Zhao Y., Yu C.C., English B.P., Gao L., Martorell A., Suk H.J., Yoshida F., DeGennaro E.M., Roossien D.H., Gong G., Seneviratne U., Tannenbaum S.R., Desimone R., Cai D, & Boyden E.S. (2016). Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nature biotechnology, 34(9), 987-992.
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6

Immunostaining of Histone H3 Phosphorylation

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Rabbit anti-phospho-Histone H3 (Ser10) antibody (Merck Millipore) was used in 1:400 dilution using the antibody staining protocol from47 . Briefly, the embryos were fixed in 4% paraformaldehyde/PBS-TT (1xPBS, 0.2% Triton X100, 0.2% Tween 20), washed five times in PBS-TT, blocked in 20% heat inactivated sheep serum (Sigma)/1% BSA in PBS-TT, and incubated with the primary antibody overnight at 4 °C. After eight 15 min PBS-TT washes, the embryos were blocked again and incubated with the goat anti-rabbit Alexa568 (Life Technologies) at 1:1000 dilution overnight. DAPI was added at the final concentration of 300 nM together with the secondary antibody to counterstain chromatin. The samples were then washed again eight times 15 min with PBS-TT, embedded in Vectashield (Vectorlabs) and imaged under a Leica SP5X CLSM.
Kraus Y., Aman A., Technau U, & Genikhovich G. (2016). Pre-bilaterian origin of the blastoporal axial organizer. Nature Communications, 7, 11694.
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7

Immunodetection of HCV Proteins

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The mouse monoclonal antibody 9E10 detecting NS5A domain III of HCV was kindly provided by Charles Rice (New York, USA). The Pex19-specific monoclonal antibody was a kind gift of Gabrielle Dodt (Tübingen, Germany). The mouse monoclonal NS3/4A antibody recognizing the NS3 helicase (2E3) was generated in cooperation with Hengli Tang (San Diego, USA). The mouse anti-MxA antibody was purchased from Georg Kochs (Freiburg, Germany). Other commercially available antibodies used in this study were mouse monoclonal antibodies recognizing β-actin (Sigma Aldrich, Germany), GAPDH (Santa Cruz Biotechnology, USA) and the HA-tag (Sigma Aldrich, Germany) as well as rabbit polyclonal antibodies recognizing MAVS (Enzo Life science, Switzerland), PMP70 (Abcam, United Kingdom) and CoxIV (Cell Signaling, Netherlands). Goat-anti-rabbit-Alexa-488, goat-anti-mouse-Alexa-488, donkey-anti-mouse-Alexa-568, goat-anti-rabbit-Alexa-568, chicken-anti-rabbit-Alexa-647 (all from Life Technologies, Germany) were used as secondary antibodies. For Western blot analysis anti-mouse and anti-rabbit antibodies, each coupled with horseradish peroxidase were used (Sigma, Germany).
Bender S., Reuter A., Eberle F., Einhorn E., Binder M, & Bartenschlager R. (2015). Activation of Type I and III Interferon Response by Mitochondrial and Peroxisomal MAVS and Inhibition by Hepatitis C Virus. PLoS Pathogens, 11(11), e1005264.
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8

Immunoblotting and Antibody Validation

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S1P was purchased from Cayman
Chemicals and JTE-013 from Sigma-Aldrich. Mouse anti-ERK 1/2 (1:1000,
clone 3A7), rabbit anti-phospho-ERK1/2 (T202/Y204) (1:1000, clone
D13.14.4E), rabbit anti-GFP (1:200, clone D5.1), rabbit anti-HA tag
(1:1000, clone C29F4), rabbit anti-p38 (1:1000, catalog no. 9212),
rabbit anti-phospho-p38 (T180/Y192) (1:1000, clone D3F9), rabbit anti-AKT
(1:1000, clone C67E7), and rabbit anti-phospho-AKT (S473) (1:1000,
clone D9E) antibodies were obtained from Cell Signaling. The mouse
anti-myc antibody (1:800, clone 9E10) was from Santa Cruz Biotechnology,
and the mouse anti-HA antibody (1:1000, clone 12CA5) was from Roche.
Rabbit anti-Flag (1:1000, clone F-7425), mouse anti-β-actin
(1:10000, clone A1978), and rabbit anti-β-catenin (1:1000, clone
C2206) antibodies were from Sigma-Aldrich. Goat anti-mouse Alexa 488
(1:1000), goat anti-rabbit Alexa 488 (1:1000), and goat anti-rabbit
Alexa 568 (1:1000) antibodies were purchased from Life Technologies.
Donkey anti-mouse IRDye800CW (1:20000) and donkey anti-mouse IRDye680LT
(1:20000) were bought from LI-COR Biosciences.
Burczyk M., Burkhalter M.D., Blätte T., Matysik S., Caron M.G., Barak L.S, & Philipp M. (2015). Phenotypic Regulation of the Sphingosine 1-Phosphate Receptor Miles Apart by G Protein-Coupled Receptor Kinase 2. Biochemistry, 54(3), 765-775.
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9

Quantifying Intestinal Bacterial Adherence

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FISH in the distal colonic section was performed as described previously38 (link), except for using 10 μg of an Alexa Fluor 488-conjugated bacterial rRNA EUB338 probe for hybridization. The epithelial region in contact with the bacteria was evaluated on a FISH-stained section, and the proportion of epithelial region in contact with bacteria was determined from a whole intestinal section in a blind fashion using NIS-Elements BR 3.2 imaging software (Nikon). For co-immunostaining, the FISH-stained slides were blocked and incubated with anti-Muc2 antibody (sc-15334, Santa Cruz Biotech.) overnight at 4 °C. The slides were further incubated with goat anti-rabbit Alexa 568 (Life Technologies), counterstained with DAPI solution (0.5 μg/ml in PBS) and visualized with a confocal microscope.
Lee S.Y., Kim H., Kim K., Lee H., Lee S, & Lee D. (2016). Arhgap17, a RhoGTPase activating protein, regulates mucosal and epithelial barrier function in the mouse colon. Scientific Reports, 6, 26923.
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10

Iba1 and YFP Fluorescence Labeling

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For fluorescence labeling of Iba1 with or without co-detection of YFP, sections were rinsed with TBS and then blocked with TBST plus 5% normal goat serum for 1 hr at RT before overnight incubation in primary antibody diluted 1:1000 in block (rabbit anti-Iba1, Wako Chemicals USA, Richmond, VA, 019-19741; 1:1000 chicken anti-GFP, Abcam). Sections were washed several times in TBS, followed by 2 hr incubation at RT in secondary antibody diluted 1:500 in block (goat anti-rabbit Alexa 568, Life Technologies; 1:500 goat anti-chicken Alexa 488, Life Technologies). Sections were again washed in TBS, incubated 10 min in 0.2 ug/ml DAPI diluted in TBS before being mounted and coverslipped.
Zhao R., Grunke S.D., Wood C.A., Perez G.A., Comstock M., Li M.H., Singh A.K., Park K.W, & Jankowsky J.L. (2022). Activity disruption causes degeneration of entorhinal neurons in a mouse model of Alzheimer’s circuit dysfunction. eLife, 11, e83813.
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