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Multiplex array kit

Manufactured by Bio-Rad
Sourced in United States

The Multiplex array kit is a laboratory equipment designed for the simultaneous detection and quantification of multiple analytes in a single sample. The kit utilizes a bead-based platform to enable multiplexed analysis, allowing researchers to obtain comprehensive data from limited sample volumes.

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2 protocols using multiplex array kit

1

Multiplex Cytokine Quantification

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Inflammatory cytokines such as interleukin (IL)-1β, IL-13 and tumor necrosis factor-alpha (TNF-α) were measured in serum by using multiplex array kit (Bio-Rad, San Diego, CA, U.S.A.) and run on Luminex technology (Bio-Plex Multiplex Bead array system TM, Bio-Rad Hercules, CA, U.S.A.) according to manufacturer’s instruction. Raw fluorescence data were analyzed by software using a 5-parameter logistic method.
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2

Multiplex Cytokine Profiling in Serum and Skin

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The levels of inflammatory cytokines such as interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α, in serum and skin lysate were measured. Moreover, the levels of Th1 cytokines including IL-2, IL-12 (p70), and interferon gamma (IFN)-γ, as well as Th2 cytokines including IL-4 and IL-10, were measured by using a multiplex array kit (Bio-Rad, San Diego, CA, USA) and run on Luminex technology (Bio-Plex Multiplex Bead Array System™, Bio-Rad, Hercules, CA, U.S.A.) according to manufacturer’s instruction. Raw fluorescence data were analyzed by using a five-parameter logistic method.
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