1010 transmission

Manufactured by JEOL

Sourced in Japan, United States

The JEOL 1010 is a transmission electron microscope (TEM) designed for imaging and analyzing samples at the nanoscale level. It provides high-resolution, bright-field imaging of thin specimens. The JEOL 1010 is capable of operating at accelerating voltages up to 100 kV.

Automatically generated - may contain errors

Lab products found in correlation

Ammonium molybdate, by Merck Group (1 mentions) Amicon ultra, by Merck Group (1 mentions) Zetasizer nano z, by Malvern Panalytical (1 mentions) Afsii, by Leica (1 mentions)

Close spelling variants of this product

JEM 1010 transmission electron microscope (291 citations) JEOL 1010 transmission electron microscope (105 citations) JEM-1010 transmission (16 citations) JEM 1010 transmission electron (4 citations) EM 1010 transmission electron microscope (2 citations) GEM-1010 Transmission electron microscope (2 citations) Jeol 1010 EX transmission electron microscope (2 citations)

11 protocols using 1010 transmission

Negative Staining of SEC Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples from SEC experiments were adsorbed to glow-discharged carbon over parlodion carbon-coated copper grids (EM Sciences). Samples were negatively stained with 0.75% uranyl formate and visualized with a JEOL 1010 transmission electron microscope equipped with a 1K×1K Gatan charge-coupled-device (CCD) camera.

Huang Q., Cai D., Yan R., Li L., Zong Y., Guo L., Mercier A., Zhou Y., Tang A., Henne K, & Colonno R. (2020). Preclinical Profile and Characterization of the Hepatitis B Virus Core Protein Inhibitor ABI-H0731. Antimicrobial Agents and Chemotherapy, 64(11), e01463-20.

+ Open protocol

+ Expand

Visualizing Dendrimer Morphology via TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

To examine dendrimer morphology, TEM was used. Ten microliters of dendrimer solution at 1 mmol/L were placed on 200-mesh carbon-coated copper grids. The sample was stained with saturated uranyl acetate for 20 min, washed in demineralised water and dried at room temperature. Images were obtained using a JEOL1010 transmission electron microscope (JEOL, Tokyo, Japan).

Michlewska S., Kubczak M., Maroto-Díaz M., Sanz del Olmo N., Ortega P., Shcharbin D., Gomez Ramirez R., Javier de la Mata F., Ionov M, & Bryszewska M. (2019). Synthesis and Characterization of FITC Labelled Ruthenium Dendrimer as a Prospective Anticancer Drug. Biomolecules, 9(9), 411.

+ Open protocol

+ Expand

Transmission Electron Microscopy of Peptide Fibrils

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peptide samples (∼20 ng)
in 1 μL of buffer were placed onto freshly glow-discharged carbon
films on 300 mesh nickel grids for 2 min and blotted with filter paper.
A 1% (w/v) solution of ammonium molybdate (Sigma-Aldrich) was adjusted
to pH 7.4 with ammonium hydroxide, applied for 2 min, blotted, and
air-dried. Images were recorded using a JEOL-1010 transmission electron
microscope (JEOL, Tokyo, Japan), operating at 80 kV, equipped with
a side-mounted CCD digital camera. All fibril preparations used for
HRF studies were examined initially by TEM to verify fibril morphology.

Klinger A.L., Kiselar J., Ilchenko S., Komatsu H., Chance M.R, & Axelsen P.H. (2014). A Synchrotron-Based Hydroxyl Radical Footprinting Analysis of Amyloid Fibrils and Prefibrillar Intermediates with Residue-Specific Resolution. Biochemistry, 53(49), 7724-7734.

+ Open protocol

+ Expand

Phage Concentration and Electron Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phage suspensions were 100-fold concentrated using Amicon Ultra 15 ml tubes (100 kDa Amicon Ultra centrifugal filter units, Millipore, Bedford, MA, United States) by centrifuging at 3,000 × g for 10 min. Ten microliter of each phage suspension were dropped onto copper grids with carbon coated Formvar films, negatively stained with 2% ammonium molybdate (pH 6.8) and examined under a Jeol 1010 transmission electron microscope (TEM) (JEOL Inc. Peabody, MA, United States) operating at 80 kV.

Zuppi M., Tozzoli R., Chiani P., Quiros P., Martinez-Velazquez A., Michelacci V., Muniesa M, & Morabito S. (2020). Investigation on the Evolution of Shiga Toxin-Converting Phages Based on Whole Genome Sequencing. Frontiers in Microbiology, 11, 1472.

+ Open protocol

+ Expand

Exosome Morphology Analysis by TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

Exosomes were placed onto a continuous carbon grid and negatively stained with 2% uranyl acetate. The morphology of the particles was examined using a JEOL 1010 transmission electron microscope (JEOL, Peabody, MA, USA).

Pillay P., Vatish M., Duarte R., Moodley J, & Mackraj I. (2019). Exosomal microRNA profiling in early and late onset preeclamptic pregnant women reflects pathophysiology. International Journal of Nanomedicine, 14, 5637-5657.

+ Open protocol

+ Expand

Characterization of Iron Oxide Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

An IONP stock sample (2 mg Fe/mL) was diluted in dH₂O and deposited on 200-mesh carbon coated copper grids (Polysciences, Warrington, PA, USA) for TEM imaging with a JEOL 1010 transmission electron microscope operating at 80 kV. Mean iron core size was determined by measuring the diameter of 100 individual nanoparticles. Imaging analysis was performed with Image J software (NIH). The stock sample of IONPs was diluted in phosphate buffered saline (pH 7.4 – Invitrogen, Carlsbad, CA, USA) for determination of hydrodynamic diameter by DLS – as recorded on a Zetasizer Nano-ZS (Malvern Instruments, UK) using the non-invasive back-scatter (NIBS) mode. Zeta potential measurements were also measured using a Zetasizer Nano-ZS.

de Barros A.L., Chacko A.M., Mikitsh J.L., Zaki A.A., Salavati A., Saboury B., Tsourkas A, & Alavi A. (2014). Assessment of global cardiac uptake of Radiolabeled Iron Oxide Nanoparticles in Apolipoprotein E-Deficient Mice: implications for imaging cardiovascular inflammation. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 16(3), 330-339.

+ Open protocol

+ Expand

Copolymer Particle Morphology Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

A JEOL 1010 transmission electron microscope (TEM, JEOL Ltd., Tokyo, Japan) was employed to observe the morphology of the copolymer particles. To accomplish this, one drop of latex was diluted up to 200 times with water, and one drop of this solution was deposited onto a copper grid and then dyed with a drop of 1.0 wt % phosphotungstic acid aqueous solution for 24 h and dried in a vacuum oven at 50 °C for 12 h.

Castellanos S.G., Fernández-Escamilla V.V., Corona-Rivera M.Á., González-Iñiguez K.J., Barrera A., Moscoso-Sánchez F.J., Figueroa-Ochoa E.B., Ceja I., Rabelero M, & Aguilar J. (2023). Coagulative Nucleation in the Copolymerization of Methyl Methacrylate–Butyl Acrylate under Monomer-Starved Conditions. Polymers, 15(7), 1628.

+ Open protocol

+ Expand

Exosome Morphology Visualization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated exosomes were immobilised on a continuous carbon grid. The grids were thereafter negatively stained with 2% uranyl acetate. The morphology of the exosomes was examined using a JEOL 1010 transmission electron microscope (JEOL, Peabody, MA, USA).

Pillay P., Moodley K., Vatish M, & Moodley J. (2020). Exosomal MicroRNAs in Pregnancy Provides Insight into a Possible Cure for Cancer. International Journal of Molecular Sciences, 21(15), 5384.

+ Open protocol

+ Expand

Ultrastructural Analysis of Macrophage Antigens

Check if the same lab product or an alternative is used in the 5 most similar protocols

Macrophage samples were high-pressure frozen in an Abra HPM-010 machine and freeze-substituted in 99% acetone, 0.1% uranyl acetate, 1% distilled water (dH₂O) in a Leica AFSII automatic freeze-substitution device. Freeze substitution was performed at −90°C for 72 h and then ramped to −50°C over 24 h, at which point cells were placed in 4-h-graded steps of 25%, 50%, 75%, 100% HM-20 in acetone. HM-20 was polymerized with 360-nm light for 48 h at −50°C and an additional 24 h at room temperature. Polymerized blocks trimmed to regions of interest were cut at 60- to 80-nm thicknesses and immunologically probed with IgG-purified type 23F rabbit serum. Protein A conjugated to 15-nm colloidal gold beads was used to secondarily detect the presence of antibody-antigen complexes. Imaging was performed on a JEOL 1010 transmission electron microscope (TEM) operating at 80 keV.

Lemon J.K, & Weiser J.N. (2015). Degradation Products of the Extracellular Pathogen Streptococcus pneumoniae Access the Cytosol via Its Pore-Forming Toxin. mBio, 6(1), e02110-14.

+ Open protocol

+ Expand

Morphological Characterization of Isolate TWA4

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolate TWA4 cell morphology was investigated by phase-contrast and electron microscopy. The Gram reaction of isolate TWA4 was determined by conventional Gram staining [43 ] and the KOH test [44 (link)]. The structure of the cell wall of isolate TWA4 was determined by transmission electron microscopy (TEM) after high pressure freezing and freeze substitution in osmium tetroxide-uranyl acetate in acetone as outlined by McDonald and Webb [45 (link)]. Thin sections were stained with uranyl acetate and lead citrate [46 (link)] and visualised using a JEOL 1010 transmission electron microscope (Jeol Ltd., Tokyo, Japan).

Gagen E.J., Wang J., Padmanabha J., Liu J., de Carvalho I.P., Liu J., Webb R.I., Al Jassim R., Morrison M., Denman S.E, & McSweeney C.S. (2014). Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach. BMC Microbiology, 14, 314.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!