Mda mb 231

Manufactured by Cobioer Biosciences

Sourced in China

The MDA-MB-231 is a cell line derived from a human breast adenocarcinoma. It is a widely used model for cancer research, particularly in the study of triple-negative breast cancer.

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Lab products found in correlation

Mcf 10a, by Cobioer Biosciences (4 mentions) Mcf 7, by Cobioer Biosciences (4 mentions) Mda mb 468, by Cobioer Biosciences (3 mentions) Bt 474, by Cobioer Biosciences (3 mentions) Bt 549, by Cobioer Biosciences (2 mentions) Hs 578t, by Cobioer Biosciences (2 mentions) Mda mb 453, by Cobioer Biosciences (2 mentions) Mda mb 157, by Cobioer Biosciences (2 mentions) Mda mb 231, by Thermo Fisher Scientific (2 mentions) Mcf 10a, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Mem medium, by Corning (1 mentions) Mda mb 468, by Corning (1 mentions) Hek293t, by Cobioer Biosciences (1 mentions) Su dhl 6, by Corning (1 mentions) Rpmi 1640 medium, by Corning (1 mentions) Hek293t, by Corning (1 mentions) Dmem medium, by Corning (1 mentions)

11 protocols using mda mb 231

Investigating circRNA and miRNA Regulation in BC

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The human BC cell lines (BT‐549 and MDA‐MB‐231) and noncancerous cell line MCF‐10A involved in the study were purchased from Cobioer. BT‐549 cell lines were cultured in RPMI‐1640 medium (Gibco) containing 10% FBS, and MDA‐MB‐231 and MCF‐10A cell lines were routinely cultured in DMEM medium (Gibco) containing 10% FBS in an incubator at 37°C and 5% CO₂.
Si‐circ_0005273 and its negative control (si‐NC). MiR‐509‐3p mimic (miR‐509‐3p), miR‐NC, miR‐509‐3p inhibitor (anti‐miR‐509‐3p), and anti‐miR‐NC, hyaluronan‐mediated motility receptor (HMMR) overexpression vector pcDNA3.0‐HMMR and blank vector control (pcDNA3.0‐NC) were provided by Ribobio. Lipofectamine 3000 reagent (Invitrogen) was used for each transfection based on the manufacturer's instructions.

Peng Y., Cui J., Ma K, & Zhong X. (2023). Hsa_circ_0005273 acts as a sponge of miR‐509‐3p to promote the malignant behaviors of breast cancer by regulating HMMR expression. Thoracic Cancer, 14(9), 794-804.

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Cultivation of Human Cancer Cell Lines

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Human breast cancer cell lines (MDA-MB-231, MDA-MB-468, and Hs578T), DLBCL cell line (Pfeiffer, Karpas 422, WSU-DLCL2 and SU-DHL6), and HEK293T were purchased from Cobioer Biosciences Co., Ltd (Nanjing, China). Pfeiffer, Karpas 422, WSU-DLCL2 and SU-DHL6 cell lines were cultured in RPMI-1640 medium (Corning, Midland, NY, USA) with 10% FBS (VivaCell, Shanghai, China), and MDA-MB-231, MDA-MB-468 and HEK293T were cultured in D MEM medium (Corning) with 10% FBS (VivaCell, Shanghai, China), Hs578T were cultured in MEM medium (Corning, Midland, NY, USA) with 10% FBS(VivaCell, Shanghai, China) and supplemented with 1% penicillin/streptomycin (v/v).

Mei H., Wu H., Yang J., Zhou B., Wang A., Hu C., Qi S., Jiang Z., Zou F., Wang B., Liu F., Chen Y., Wang W., Liu J, & Liu Q. (2023). Discovery of IHMT-337 as a potent irreversible EZH2 inhibitor targeting CDK4 transcription for malignancies. Signal Transduction and Targeted Therapy, 8, 18.

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Investigating Breast Cancer Cell Lines

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Breast cancer cell lines BT-474, MCF7, Hs-578-T, MDA-MB-231, MDA-MB-453, and MDA-MB-468 (Cobioer, Nanjing, China) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Sunncell, Wuhan, China) with 10% fetal bovine serum (FBS) and certain cell growth factor. BT-549, HCC1937, and T47D cell lines were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Sunncell, Wuhan, China) with 10% or 20% FBS.
For the following function experiments, T47D, MCF7, MDA-MB-453, HCC1937, MDA-MB-231, and MDA-MB-468 cells were treated with 100 or 500 nM GDC-0941 (PI3K selective inhibitor, MedChemExpress, New Jersey, USA) or dimethyl sulfoxide (DMSO) for 24 h. In addition, MDA-MB-231 and HCC1937 cells were treated with 100 nM OSU-T315 (ILK inhibitor, SolelyBio, Beijing, China), tumor necrosis factor (TNF)-α, IgG, or TNF-α antibody (ab6671, Abcam, Cambridge, UK).
In addition, MCF7 and MDA-MB-453 cells were transfected with ILK, and MDA-MB-231 and HCC1937 cells were transfected with shILK.

Chen H., Cheng M., Gao P., Zhang X., Li G., Wang L., Qin L, & Li H. (2022). GDC-0941 activates integrin linked kinase (ILK) expression to cause resistance to GDC-0941 in breast cancer by the tumor necrosis factor (TNF)-α signaling pathway. Bioengineered, 13(4), 10944-10955.

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Breast Cell Line Transfection Protocol

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Human normal breast epithelial cell line MCF 10A (CBP60419) and breast cancer cell lines AU565 (CBP60353), MCF-7 (CBP60380), MDA-MB-157 (CBP60381) and MDA-MB-231 (CBP60382) were all purchased from Cobioer (Nanjing, China). Plasmids including si-NC, si-LINC00461, inhibitor NC, miR-144-3p inhibitor, mimic NC, miR-144-3p mimic, oe-NC, oe-KPNA2 were ordered from GenePharma (Shanghai, China), and the siRNAs were sequenced as below: si-LINC00461: 5′-CTGCAAAGAAGCATAAAATGA-3′; si-NC: 5′-TTCTCCGAACGTG TCACGT-3′.
Before transfection, cells were grown in 6-well plates (3 × 10⁵ cells/well) until the density reached 50%. 250 μL of serum-free Opti-MEM (51985042, Gibco, Gaitherburg, MD, USA) was used to dilute the target plasmids (4 μg) and Lipofectamin 2000 reagent (10 μL; 11668-019, Invitrogen, NY, California, USA), respectively. Thereafter, the dilutions were mixed, and then dripped into cells after 20 min. All cells were maintained in 5% CO₂ at 37 °C for 6 h, and cultured for additional 36–48 h with fresh mediums.

Zhang Q., Jin X., Shi W., Chen X., Pang W., Yu X, & Yang L. (2020). A long non-coding RNA LINC00461-dependent mechanism underlying breast cancer invasion and migration via the miR-144-3p/KPNA2 axis. Cancer Cell International, 20, 137.

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Culturing Normal and Breast Cancer Cells

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Normal MCF‐10A cells and BC cell lines (MCF‐7, MDA‐MB‐231, BT‐549, and T47D) were purchased from COBIOER. BC cell lines were cultured in the RPMI‐1640 medium (COBIOER) plus 1% penicillin–streptomycin (COBIOER) and 10% fetal bovine serum (FBS, COBIOER). MCF‐10A cells were grown in MEBM BulletKit. All medium was maintained at 37°Cwith 5% CO₂.

Liu Y., Liu Y., Luo J., Zhao W., Hu C, & Chen G. (2022). Hsa_circ_0002082 up‐regulates Centromere Protein F via abolishing miR‐508‐3p to promote breast cancer progression. Journal of Clinical Laboratory Analysis, 36(11), e24697.

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Culturing Breast Cancer Cell Lines

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Human TNBC line MDA-MB-231, luminal B type of breast cancer cell-line BT474, luminal A type of breast cancer cell-line T47D and MCF-7, and MCF-10A cells (human mammary epithelial cells) were purchased from COBIOER (Nanjing, China). L-15 medium (Thermo Fisher Scientific, Waltham, MA, USA) and DMEM medium with 10% FBS (fetal bovine serum, Thermo Fisher) were used to culture MDA-MB-231 and other cell lines at 37°C under a circumstance containing 5% CO₂, respectively.

Yang B., Wang F, & Zheng G. (2021). Transmembrane protein TMEM119 facilitates the stemness of breast cancer cells by activating Wnt/β-catenin pathway. Bioengineered, 12(1), 4856-4867.

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Antibody Fragment Conjugation in Breast Cancer

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The HER2⁺ breast cancer cell line (BT-474) and the HER2⁻ breast cancer cell line (MDA-MB-231) were purchased from Cobioer (Nanjing, People’s Republic of China).
F(ab′)₂ fragments of TMAB were produced by the digestion of TMAB using immobilized pepsin, according to the manufacturer’s protocol. The disulfide bonds in F(ab′)₂ were reduced with 20 mM cysteine to produce Fab′, which contains one terminal free thiol group per Fab′ molecule, for use in conjugation protocols.
Female BALB/c mice of 6–8 weeks were purchased from Charles River Laboratories (Beijing Vital River Laboratory Animal Technology Co., Ltd, Beijing, People’s Republic of China). Sprague-Dawley rats (180–200 g) were kindly provided by the Luye Pharma Group (Shandong, People’s Republic of China). All animal studies were performed according to the Guide for the Care and Use of Laboratory Animals. The Animal Ethics Committee of Yantai University approved the study.

Duan D., Wang A., Ni L., Zhang L., Yan X., Jiang Y., Mu H., Wu Z., Sun K, & Li Y. (2018). Trastuzumab- and Fab′ fragment-modified curcumin PEG-PLGA nanoparticles: preparation and evaluation in vitro and in vivo. International Journal of Nanomedicine, 13, 1831-1840.

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Ovarian and Breast Cancer Tissue and Cell Line Acquisition

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Archival FFPE tumor tissues were obtained from 195 ovarian and breast cancer patients who had signed the informed consents, and the study was approved by the Institutional Review Board of BGI Co., Ltd. 17 human cancer cells (HCC38, HCC1428, HCC1143, HCC1806, MX-1, HCC70, ZR-75-1, MDA-MB-453, MDA-MB-231, MDA-MB-361, MDA-MB-415, ZR-75-30, HS-578T, IGR-OV1, A2780, NCI/ADR-RES and OVCAR-4) and 3 matched-wildtype cell lines (HCC38BL, HCC1143BL and HCC1428BL) were purchased from the CoBioer Biosciences Co., Ltd. All cell lines have been verified by STR and were provided in the form of DNA status.

Chen D., Shao M., Meng P., Wang C., Li Q., Cai Y., Song C., Wang X, & Shi T. (2021). GSA: an independent development algorithm for calling copy number and detecting homologous recombination deficiency (HRD) from target capture sequencing. BMC Bioinformatics, 22, 562.

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Xenograft Tumor Models of Breast Cancer

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The following cell lines were obtained from Cobioer (Cobioer Biosciences Co., Ltd., Nanjing, China): MDA-MB-231, T47D, MDA-MB-468, MDA-MB-157, MCF-7 and MCF-10A. Human breast cancer cell lines MDA-MB-231, T47D, MDA-MB-468, MDA-MB-157 and MCF-7 were preserved in RPMI 1640 supplemented with 10% FBS and were growth in a 37℃ humidi ed chamber with 5% CO 2 . MCF10A cells were cultured in Dulbecco's modi ed Eagle's medium/F12 with 10% horse serum, 20 ng/ml epidermal growth factor, 0.5 ug/ml hydrocortisone, 10 ug/ml insulin, 100 ng/ml cholera toxin. The passage number for each cell line was less than 15 when the experiments were performed. Female BALB/c nude mice, 6 to 8 weeks old, were purchased from Guangdong Medical Animal Experimental Center (Guangdong, China). Animal experimental procedures comply with the Care and Use of Laboratory Animals Guide and approved by the Animal Experimental Ethics Committee of Southern Medical University. MDA-MB-468, T47D and MCF-7 cells (1.0 × 10 7 in 0.2 ml PBS) were subcutaneously injected into the right side of the posterior anks of nude mice (n = 5), respectively. When tumor volumes reached 1000 mm 3 , mice were sacri ced by exposure to carbon dioxide and tumors were excised.

Wu H., Lian H., Chen Q., Yang J., Ou B., Quan D., Zhou L., Lv L., Liu M., & Wu S. (2020). Identification of Seven Hub Genes as the Novel Biomarkers in Triple-negative Breast Cancer and Breast Cancer Metastasis.

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Culturing and Maintaining Triple-Negative Breast Cancer Cell Lines

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The human SUM149 and MDA-MB-231 cell lines (TNBC) and murine 4T1-luc cells (TNBC, highly metastatic) were obtained from Cobioer (Nanjing, China). SUM149 cells were maintained in Ham's F12 medium supplemented with 5% (v/v) fetal bovine serum (FBS), 5 μg/mL of insulin, 1 μg/mL of hydrocortisone, 100 U/mL of penicillin, and 100 μg/mL of streptomycin. MDA-MB- 231 cells were maintained in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% (v/v) FBS, 100 U/mL penicillin, and 100 mg/L streptomycin. The 4T1-luc cells were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS, 100 U/mL penicillin, and 100 mg/L streptomycin. The cells were incubated at 37°C in a 5% CO₂ incubator with saturated humidity.

Wang Y., Zhao L., Han X., Wang Y., Mi J., Wang C., Sun D., Fu Y., Zhao X., Guo H, & Wang Q. (2020). Saikosaponin A Inhibits Triple-Negative Breast Cancer Growth and Metastasis Through Downregulation of CXCR4. Frontiers in Oncology, 9, 1487.

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