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P24 ab9071

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P24 (ab9071) is a protein that is used as a marker for the detection of human immunodeficiency virus (HIV) infection. It is a core structural protein of the HIV virus and can be used to determine the presence and stage of HIV infection.

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Lab products found in correlation

Cd4 ab133616 and, by Abcam (2 mentions) Apc anti human tetherin 348410, by BioLegend (2 mentions) Hla c antibody mabf233, by Merck Group (2 mentions) Snat1 antibody sc 67080, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 488 conjugated anti mouse igg2b antibody a 21141, by Thermo Fisher Scientific (2 mentions) Mouse igg2b isotype control ma5 14447, by Thermo Fisher Scientific (2 mentions) Gapdh antibody 14c10, by Cell Signaling Technology (2 mentions) Apc mouse igg1 isotype control, by BioLegend (2 mentions) Mouse and rabbit hrp conjugated antibodies, by Abcam (2 mentions) β actin ac 74, by Merck Group (1 mentions) Ibright cl1000 imaging system, by Thermo Fisher Scientific (1 mentions) Supersignal west pico, by Thermo Fisher Scientific (1 mentions) Goat anti mouse hrp, by Jackson ImmunoResearch (1 mentions) Goat anti rabbit hrp, by Jackson ImmunoResearch (1 mentions)

3 protocols using p24 ab9071

1

Immunoblotting Antibody Panel for Diverse Proteins

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Antibodies against the following proteins were used for immunoblot, listed by manufacturer: Atlas antibodies: TASOR (HPA006735). Bethyl: BBX (A303-151A), HLTF (A300-230A), RALY (A302-070A), ZNF512B (A303-234A). Cell Signaling Technology: SMN1/2 (2F1). Novus: ESCO2 (NB100-87021). Origene: UNG2 (2C12). Proteintech: Vpr (51143-I-AP). Santa Cruz: CCNB1 (SC-245), ZGPAT (SC-515524). Sigma: β-actin (AC74). Abcam: p24 (ab9071), VCP (ab11433). The following secondary antibodies were used: goat anti-mouse-HRP and goat anti-rabbit-HRP (immunoblot, Jackson ImmunoResearch, West Grove, PA). Blots were immersed in chemiluminescent substrate (SuperSignal West Pico or Dura, Thermo Fisher Scientific), and signal was visualized using X-ray film or the iBright CL1000 imaging system (Thermo Fisher Scientific).
Greenwood E.J., Williamson J.C., Sienkiewicz A., Naamati A., Matheson N.J, & Lehner P.J. (2019). Promiscuous Targeting of Cellular Proteins by Vpr Drives Systems-Level Proteomic Remodeling in HIV-1 Infection. Cell Reports, 27(5), 1579-1596.e7.
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2

Comprehensive Antibody Panel for HIV-1 Analysis

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CD4 (ab133616) and p24 (ab9071) antibodies were purchased from Abcam. APC anti-human tetherin (348410) and APC mouse IgG1 isotype control were from Biolegend. HLA-C antibody (MABF233) was from Millipore. SNAT1 antibody (sc-67080) was from Santa Cruz. GAPDH antibody (14C10) was from cell signaling. Mouse and rabbit HRP-conjugated antibodies were from Abcam. Alexa Fluor 488 conjugated anti-mouse IgG2b antibody (A-21141) and mouse IgG2b isotype control (MA5-14447) were from ThermoFisher Scientific. Polyclonal antibody against a conserved region of HIV-1 Vpu (both subtype A and B) was raised in mouse by conjugating KLH (keyhole limpet hemocyanin) to a synthetic peptide (ERAEDSGNESDG) corresponding to amino acids 48–59 of Vpu.
Romani B., Kavyanifard A, & Allahbakhshi E. (2017). Functional conservation and coherence of HIV-1 subtype A Vpu alleles. Scientific Reports, 7, 87.
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3

Comprehensive Antibody Panel for HIV-1 Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD4 (ab133616) and p24 (ab9071) antibodies were purchased from Abcam. APC anti-human tetherin (348410) and APC mouse IgG1 isotype control were from Biolegend. HLA-C antibody (MABF233) was from Millipore. SNAT1 antibody (sc-67080) was from Santa Cruz. GAPDH antibody (14C10) was from cell signaling. Mouse and rabbit HRP-conjugated antibodies were from Abcam. Alexa Fluor 488 conjugated anti-mouse IgG2b antibody (A-21141) and mouse IgG2b isotype control (MA5-14447) were from ThermoFisher Scientific. Polyclonal antibody against a conserved region of HIV-1 Vpu (both subtype A and B) was raised in mouse by conjugating KLH (keyhole limpet hemocyanin) to a synthetic peptide (ERAEDSGNESDG) corresponding to amino acids 48–59 of Vpu.
Romani B., Kavyanifard A, & Allahbakhshi E. (2017). Functional conservation and coherence of HIV-1 subtype A Vpu alleles. Scientific Reports, 7, 44894.
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