Mda mb 231

Manufactured by Solarbio

Sourced in China

MDA‐MB‐231 is a human breast adenocarcinoma cell line. It is commonly used in cancer research to study the behavior and characteristics of triple-negative breast cancer cells.

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6 protocols using mda mb 231

Culturing Diverse Cell Lines with FBS

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Cells (MCF‐10A, MCF‐7, MDA‐MB‐231, and 293T) were purchased from the American Type Culture Collection (ATCC, Manassas, Virginia, USA). MCF‐10A cells were cultured in mammary epithelial growth medium (MEGM) (Catalog No. CC‐3150). MCF‐7 were cultured in Eagle's Minimum Essential Medium (EMEM) (ATCC, 30‐2003), with MDA‐MB‐231 in Roswell Park Memorial Institute‐1640 solution (RPMI) (Solarbio, Catalog No. 3180) and 293T in Dulbecco's modified Eagle’s medium (DMEM) (Solarbio, Catalog No.11995).
All four kinds of cells were cultured at 37°C with 5% CO₂, and their media was supplemented with 10% fetal bovine serum (FBS).

Zhang L., Liu Q., Mu Q., Zhou D., Li H., Zhang B, & Yin C. (2020). MiR‐429 suppresses proliferation and invasion of breast cancer via inhibiting the Wnt/β‐catenin signaling pathway. Thoracic Cancer, 11(11), 3126-3138.

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Culturing Diverse Breast Cell Lines

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Human normal breast epithelial cells (MCF-10A) and BRCA cell lines (MCF-7, MDA-MB-231, MDA-MB-468, HCC1954, HCC38, SK-BR3, and T47D) were obtained from American Typical Culture Collection (ATCC, Manassas, VA). Normal breast MCF-10A cells were cultured in a mammary epithelium-specific medium (Lonza). MDA-MB-231 and MDA-MB-468 cells were cultured in sealed medium containing L-15 basal medium (Solarbio). MCF-7 cells were cultured in DMEM medium (Gibco). HCC1954, HCC38, SK-BR3, and T47D cells were cultured in 1640 medium (Gibco). In addition to the above dedicated medium, the addition of 10% fetal bovine serum (VivaCell) was essential, while penicillin/streptomycin (Hyclone) was selectively added according to the cell status. Growth conditions for all cells were 37°C and 5% CO₂ concentration.

Qu W., Yao Y., Liu Y., Jo H., Zhang Q, & Zhao H. (2023). Prognostic and Immunological Roles of CES2 in Breast Cancer and Potential Application of CES2-Targeted Fluorescent Probe DDAB in Breast Surgery. International Journal of General Medicine, 16, 1567-1580.

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IMUP Knockdown in Breast Cancer Cells

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Human BC cell lines (MDA-MB-231, MCF-7, T47D, MDA-MB-436, MDA-MB-453) and normal mammary epithelial cell line (MCF-10A) were purchased from the Cell Bank of the Shanghai Chinese Academy of Sciences (Shanghai, China). Under 5% CO2, MDA-MB-231, MCF-7, and T47D were cultured in DMEM medium (Gibco, Invitrogen, Carlsbad, CA, USA), and the MCF-10A was cultured in DMEM-F12 medium (Gibco). MDA-MB-436 and MDA-MB-453 were incubated in L-15 medium (Gibco) without CO2. All cell lines were grown with 10% Fetal bovine serum (Gibco) and 1% penicillin/streptomycin (Solarbio, Beijing, China) at 37°C.
The cells (MCF-7 and MDA-MB-231) were seeded in 6-well plates before transfection at a certain density (15× 10⁴ cells per well). Small interfering RNA (siRNA) designed and supplied by Gene Pharma (Shanghai, China) was used to silence the expression of IMUP. After premixing with Lipofectamine RNAiMAX (Invitrogen, Grand Island, NY, USA) (siRNA: iMAX= 10 ul/4 ul; 100 nM final concentration per well), the siRNA was introduced into cells. The medium was replaced after 7 h and further experiments could be conducted after 48 h. The siRNA sequences targeted IMUP were as follows: siRNA1- sense: GGUCCGGGUCCAAAGCAAGTT/antisense: CUUGCUUUGGACCCGGACCTT; siRNA2- sense: GGAUGUGAAGUCCCACGCUTT/antisense: AGCGUGGGACUUCACAUCCTT.

Wen J., Lin L., Lin B., Xia E., Qu J, & Wang O. (2020). Downregulation of Immortalization-Upregulated Protein Suppresses the Progression of Breast Cancer Cell Lines by Regulating Epithelial–Mesenchymal Transition. Cancer Management and Research, 12, 8631-8642.

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Culturing Breast Cancer Cell Lines

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The MDA-MB-231, MDA-MB-453 and MDA-MB-468 human BC cell lines were incubated in L15 medium (LA9510; Beijing Solarbio Science & Technology Co., Ltd.) containing 10% FBS (SH30084.03; HyClone; Cytiva), 1% penicillin (C8251; Beijing Solarbio Science & Technology Co., Ltd.) and 1% streptomycin (S8290; Beijing Solarbio Science & Technology Co., Ltd.) in a 37°C, 5% CO₂ incubator. The MCF-7 and ZR-75-1 cells were cultured with Minimum Essential Medium (MEM; 41500; Beijing Solarbio Science & Technology Co., Ltd.) containing 10% FBS, 1% penicillin (C8251; Beijing Solarbio Science & Technology Co., Ltd.) and 1% streptomycin (S8290; Beijing Solarbio Science & Technology Co., Ltd.) and RPMI-1640 (31800; Beijing Solarbio Science & Technology Co., Ltd.) medium containing 10% FBS, 1% penicillin (C8251; Beijing Solarbio Science & Technology Co., Ltd.) and 1% streptomycin (S8290; Beijing Solarbio Science & Technology Co., Ltd.) in a 37°C, 5% CO₂ incubator, respectively. The MCF-10A cells were cultured in Mammary Epithelium Basal Medium (CC-3150; iCell Bioscience, Inc.) in a 37°C, 5% CO₂ incubator. The MDA-MB-231, MDA-MB-453, MDA-MB-468, MCF-7 and MCF-10A cells were purchased from iCell Bioscience, Inc. The ZR-75-1 cells were purchased from Procell Life Science & Technology Co., Ltd.

Yang Y., Wu Y., Hou L., Ge X., Song G, & Jin H. (2022). Heat shock protein 20 suppresses breast carcinogenesis by inhibiting the MAPK and AKT signaling pathways. Oncology Letters, 24(6), 462.

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Culturing Breast Cancer Cell Lines

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Human breast cancer cell lines MCF‐7 and MDA‐MB‐231 and human normal breast epithelial cell line MCF‐10A were acquired from ATCC (Manassas, VA, USA), and the human breast cancer cell line ZR‐75‐1 was obtained from the cell bank of Shanghai Institutes for Biological Sciences of the Chinese Academy of Sciences (Shanghai, China). MCF‐7 cells were maintained in Eagle's minimum essential medium (EMEM; ATCC) with 0.01 mg/mL insulin (Solarbio, Beijing, China) and 10% FBS, ZR‐75‐1 cells were cultured in RPMI 1640 medium with 10% FBS, MDA‐MB‐231 were cultured in L‐15 medium with 10% FBS using impermeable flasks, and MCF‐10A cells were cultured in DMEM/F12 medium with 5% horse serum, 100 ng/mL cholera, 10 μg/mL insulin, 20 ng/mL epidermal growth factor (EGF) toxin and 500 ng/mL hydrocortisone. All cells were cultured in a humidified incubator at 37°C with 5% CO₂ (Thermo Fisher Scientific, Waltham, MA, USA).

Wang M., Wang M., Wang Z., Yu X., Song Y., Wang C., Xu Y., Wei F., Zhao Y, & Xu Y. (2018). Long non‐coding RNA‐CTD‐2108O9.1 represses breast cancer metastasis by influencing leukemia inhibitory factor receptor. Cancer Science, 109(6), 1764-1774.

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Cell Culture Conditions for Cancer and HEK293T

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MCF‐10A, MCF‐7, MDA‐MB‐231, and HEK‐293 T cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). MCF‐10A cells were cultured in mammary epithelial growth medium (Catalog no. CC‐3150). MCF‐7 and HEK‐293 T cells were cultured in Dulbecco's Modified Eagle Medium (Solarbio, Catalog no. 11995) and 1% sodium pyruvate. MDA‐MB‐231 cells were cultured in Roswell Park Memorial Institute 1640 medium (RPMI‐1640) (Solarbio, Catalog no. 3180). The four kinds of cells were cultured with 10% fetal bovine serum (FBS) (HyClone, SH30070.03) in a humidified atmosphere of 5% CO₂ at 37°C.

Yang K., Li D., Jia W., Song Y., Sun N., Wang J., Li H, & Yin C. (2022). MiR‐379‐5p inhibits the proliferation, migration, and invasion of breast cancer by targeting KIF4A. Thoracic Cancer, 13(13), 1916-1924.

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