Ab3673

The following primary antibodies were used in the present study: rabbit anti-Pan-Calcineurin A (2614, CST, USA), rabbit anti-Calcineurin A (ab3673, Abcam, USA), rabbit anti-β-Actin (4970, CST, USA), mouse anti-NF-200 (MAB5262, Chemicon, USA), mouse anti-GFAP (MAB3402, Chemicon, USA), goat anti-CGRP (ab36001, Abcam, USA), and lectin from Bandeiraea Simplicifolia-BSI-B4 FITC conjugate (L2895, Sigma-Aldrich, USA). The secondary antibodies included fluoresce in (FITC) Affinipure donkey anti-mouse IgG (715-095-150, Jackson ImmunoResearch, USA), Alex Fluor 488 AffiniPure donkey anti-goat IgG (705-545-003, Jackson ImmunoResearch, USA) and Cy3-conjugated donkey anti-rabbit IgG (711-165-152, Jackson ImmunoResearch, USA). The drugs included ulinastatin (Techpool Company, Guangdong, China) and exogenous CN (BML-SE163-5000, Enzo LifeSciences, USA). IL-10 ELISA kits (R1000, R&D Systems Inc, MN, USA) were used to measure IL-10.

Cell culture reagents: FBS (cat. no. SH30087.01; Hyclone; GE Healthcare Life Sciences); DMEM-high glucose culture medium (50 mM; cat. no. 11965-092; Gibco; Thermo Fisher Scientific, Inc.); DMEM-low glucose culture medium (5 mM; cat. no. 10567-014; Gibco; Thermo Fisher Scientific, Inc.). H9C2 cells were rat embryonic cardiomyocytes purchased from the Cell Resource Center of Shanghai Academy of Life Sciences, Chinese Academy of Sciences; Norvasc was obtained from Sigma-Aldrich (Merck KGaA); ELISA assay kit of CaN (cat. no. E-EL-R0134c) was purchased from Elabscience Biotechnology Co., Ltd.; Fluo-3 AM (calcium ion fluorescent probe; cat. no. S1056) was from Beyotime Institute of Biotechnology; DNase I (RNase-free) was purchased from Beijing Transgen Biotech Co., Ltd.; reverse transcription kit and real-time PCR kit were obtained from Vazyme; CnAβ, nuclear factor of activated T cells 3 (NFAT3), β type myosin heavy chain (β-MHC) and b-actin primary antibodies (cat. nos. ab3673, ab66781, ab207926 and ab8227, respectively) were all purchased from Abcam.