8 well chamber slide

Manufactured by Merck Group

Sourced in United States, Germany, Ireland, United Kingdom

The 8-well chamber slides are a laboratory equipment product. They provide a convenient platform for cell culture, microscopy, and other applications that require a multi-well setup. The slides feature eight individual wells that can accommodate cells or other samples, allowing for parallel experimentation or observation.

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Lab products found in correlation

Vectashield hardset mounting medium with dapi, by Vector Laboratories (4 mentions) Dead end tunel kit, by Promega (3 mentions) Laser scanning microscope 510, by Zeiss (2 mentions) Triton x 100, by Merck Group (2 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (2 mentions) Nerve growth factor (ngf), by Thermo Fisher Scientific (2 mentions) Glycergel aqueous mounting medium, by Agilent Technologies (1 mentions) Hmsc adipogenic differentiation medium bulletkit, by Lonza (1 mentions) 6 well plate, by Corning (1 mentions) Stempro adipogenesis differentiation kit, by Thermo Fisher Scientific (1 mentions) 410 mt 025 cf, by R&D Systems (1 mentions) Detection reagents red, by Merck Group (1 mentions) Duolink in situ fluorescence kit, by Merck Group (1 mentions) Isotype matched control antibodies, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 555 conjugate, by Thermo Fisher Scientific (1 mentions) Axio observer 7 microscope, by Zeiss (1 mentions) Duolink kit, by Olink (1 mentions) 4 6 diamidino 2 phenylindole dapi, by Agilent Technologies (1 mentions) Lsm 700, by Zeiss (1 mentions) Alexa fluor 488 or 594, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Chamber slides (45 citations) 8-chamber slides (9 citations)

45 protocols using 8 well chamber slide

Adipogenic Cell Differentiation Protocol

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For adipogenic cell differentiation experiments, 2 × 10⁴ cells were plated on 8-well chamber slides (Millipore) for histology and 10⁵ cells were plated on 6-well plates (Costar Corning Incorporated) for molecular analysis. Cells were grown for 21 days in hMSC Adipogenic Differentiation BulletKit Medium (Lonza) or StemPro Adipogenesis Differentiation Kit (Gibco, Thermo Fisher Scientific) and 20%FBS/DMEM (as the control). For histological analysis, cells were fixed with 4% paraformaldehyde (Sigma-Aldrich Química S.A.) before Oil Red O staining was performed. Slides were mounted with Glycergel aqueous mounting medium (Dako, Agilent Technologies Spain S.L.).

Piñeiro-Ramil M., Castro-Viñuelas R., Sanjurjo-Rodríguez C., Rodríguez-Fernández S., Hermida-Gómez T., Blanco-García F.J., Fuentes-Boquete I, & Díaz-Prado S. (2020). Immortalizing Mesenchymal Stromal Cells from Aged Donors While Keeping Their Essential Features. Stem Cells International, 2020, 5726947.

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TNFα Regulates Hepatic CYP Genes

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Primary hepatocytes cultured in the collagen I-coated 6-well plates or 8-well chamber slides (Millipore, Sigma) were treated with PBS or 25 ng/ml of recombinant murine TNFα (aa 80-235) protein (R&D system, 410-MT-025/CF) for 24 h or 48 h, followed by qRT-PCR analysis of the Cyp gene expression or BODIPY staining, respectively.

Wang G., Li J., Bojmar L., Chen H., Li Z., Tobias G.C., Hu M., Homan E.A., Lucotti S., Zhao F., Posada V., Oxley P.R., Cioffi M., Kim H.S., Wang H., Lauritzen P., Boudreau N., Shi Z., Burd C.E., Zippin J.H., Lo J.C., Pitt G.S., Hernandez J., Zambirinis C.P., Hollingsworth M.A., Grandgenett P.M., Jain M., Batra S.K., DiMaio D.J., Grem J.L., Klute K.A., Trippett T.M., Egeblad M., Paul D., Bromberg J., Kelsen D., Rajasekhar V.K., Healey J.H., Matei I.R., Jarnagin W.R., Schwartz R.E., Zhang H, & Lyden D. (2023). Tumor extracellular vesicles and particles dysregulate liver metabolism. Nature, 618(7964), 374-382.

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Apoptosis Evaluation via TUNEL Assay

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For cell staining, cells were plated on 8-well chamber slides (Millipore, Billerica, MA) and subjected to H₂O₂ treatment as described above. The terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end-labeling (TUNEL) staining for apoptotic nuclei was performed using DEAD End TUNEL kit (Promega, Madison, WI) according to the manufacturer’s instructions with minor modifications. Briefly, cells were fixed in 4 % paraformaldehyde for 25 min and then treated with permeabilization solution (0.2 % Triton X-100 solution in PBS) for 5 min at room temperature. Labeling reactions were performed with 100 µL of reaction buffer for 60 min at 37 °C in a humidified chamber, followed by steptavidin Alexa Fluor 488 or 555 conjugate (1:400, Life Technologies, Carlsbad, CA) staining. Slides were mounted using VECTASHIELD HardSet Mounting Medium with DAPI (Vector Laboratories, Burlingame, CA) and imaged using a Zeiss 510 Laser Scanning Microscope (Carl Zeiss, Thornwood, NY). Apoptosis was evaluated as the average number of TUNEL-positive cells per DAPI labeled cells at high-power magnification (×250).

Zhang L., Zhou M., Wang Y., Huang W., Qin G., Weintraub N.L, & Tang Y. (2014). miR-92a inhibits vascular smooth muscle cell apoptosis: role of the MKK4–JNK pathway. Apoptosis : an international journal on programmed cell death, 19(6), 975-983.

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EGFR-HER3 Protein Interaction Assay

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HP and Ctx^R clones were grown on 8-well chamber slides (Millipore) and processed for PLA using the DuoLink In Situ Fluorescence kit with red detection reagents (Sigma) as per the manufacture’s instructions. Primary antibodies used: EGFR (SC-03_Rabbit) and HER3 (SC-203_mouse).

Brand T.M., Iida M., Corrigan K.L., Braverman C.M., Coan J.P., Flanigan B.G., Stein A.P., Salgia R., Rolff J., Kimple R.J, & Wheeler D.L. (2017). The receptor tyrosine kinase AXL mediates nuclear translocation of the epidermal growth factor receptor. Science signaling, 10(460), eaag1064.

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Visualizing DDX17 Knockdown Impact

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HeLa cells were seeded in 8-well chamber slides (Millipore). Cells were sequentially transfected with siControl or siDDX17 and then a second round of siRNA together with pLAI, with or without increasing concentrations of siDDX17 rescue expressor construct. Cells were harvested 48 h post-co-transfection and fixed with 4% paraformaldehyde followed by washing with PBS and permeabilizing with 0.1% Triton X-100 for 10 min at room temperature. Cells were then treated with 5% BSA blocking solution for 45 min, followed by incubation with the relevant primary antibodies (Gag, Isotype matched control or DDX17) for 1 h. Cells were washed with PBST prior to incubation with the relevant secondary antibodies. Secondary antibodies used were as follows: Alexa-Fluor 647 donkey anti-mouse conjugated secondary antibody, Alexa Fluor 555 goat anti-rabbit, or isotype-matched control antibodies (Santa Cruz). Vectashield with 4′,6-diamidino-2-phenlindole (DAPI) (Vector) was applied before coverslip was mounted. Cells were visualized using Leica Sp5 confocal laser microscope. All images were digitally recorded and merged using the Leica software.

Sithole N., Williams C.A., Vaughan A.M., Kenyon J.C, & Lever A.M. (2018). DDX17 Specifically, and Independently of DDX5, Controls Use of the HIV A4/5 Splice Acceptor Cluster and Is Essential for Efficient Replication of HIV. Journal of Molecular Biology, 430(18Part B), 3111-3128.

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Apoptosis Evaluation Using TUNEL Assay

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For cell staining, cells were plated on 8-well chamber slides (Millipore, Billerica, MA) and subjected to oxidative stress treatments as described above. (1) The terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick-end-labeling (TUNEL) staining for apoptotic nuclei was performed using DEAD End TUNEL kit (Promega, Madison, WI) according to the manufacturer’s instruction with modification. Briefly, cells were fixed in 4% paraformaldehyde for 25 min and then treated with permeabilization solution (0.2% Triton X-100 solution in PBS) for 5 min at room temperature. Labeling reactions were performed with 100 μl of reaction buffer for 60 min at 37°C in a humidified chamber, followed by steptavidin Alexa Fluor 555 conjugate (1:400, Life Technologies, Carlsbad, CA) staining. Slides were mounted using VECTASHIELD HardSet Mounting Medium with DAPI (Vector Laboratories, Burlingame, CA). The staining was analyzed by Zeiss 510 Laser Scanning Microscope (Carl Zeiss, Thornwood, NY). Apoptosis was evaluated as the average number of positively stained cells per DAPI labeled cells.

Zhang L., Zhou M., Qin G., Weintraub N.L, & Tang Y. (2014). MiR-92a regulates viability and angiogenesis of endothelial cells under oxidative stress. Biochemical and biophysical research communications, 446(4), 952-958.

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Immunoblotting and Immunofluorescence Assays with ARPE-19 Cells

Cited 2 times

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ARPE-19 cells were planted into 6-well plates for immunoblotting, and were grown on 8-well chamber slides (Millipore, Billerica, MA, USA) for immunofluorescence. Cells were maintained in complete medium, and were harvested at 72 h post transfection. Immunoblotting and immunofluorescence were conducted using a previously described protocol^{60 (link),61 (link)}. Antibodies were detailed in Supplementary Table S3. ImageJ software (https://imagej.nih.gov/ij/index.html) was applied to determine and quantify protein expressions.

Jiang C., Xie P., Sun R., Sun X., Liu G., Ding S., Zhu M., Yan B., Liu Q., Chen X, & Zhao C. (2018). c-Jun-mediated microRNA-302d-3p induces RPE dedifferentiation by targeting p21Waf1/Cip1. Cell Death & Disease, 9(5), 451.

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In Situ Protein Interaction Assay

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About 20,000 cells were seeded per well in 8-well chamber slides (Millipore) and grown for 2 days with Dox (5 μg/mL) induction. After the indicated treatment, the cells were fixed with 4% PFA for 15 min in the dark at RT, permeabilized in 0.2% Triton X-100 (Sigma) in 1× PBS for 10 min at RT, washed with PBS, blocked with 100 mM glycine, and washed again with PBS. The in situ PLA experiment was performed using the DuoLink kit (Olink Biosciences, Uppsala, Sweden) following the manufacturer’s instructions. The following primary antibodies were used at the specified dilutions: mouse anti-FLAG at 1:1000; rabbit anti-α-Syn at 1:200, and rabbit anti-histone 3 at 1:200 dilution. The nuclear background of cells was visualized by staining with 4’,6-diamidino-2-phenylindole (DAPI; Agilent Technologies). After staining, cells were examined with a Zeiss Axio Observer 7 microscope.

Vasquez V., Mitra J., Hegde P.M., Pandey A., Sengupta S., Mitra S., Rao K.S, & Hegde M.L. (2017). Chromatin-Bound Oxidized α-Synuclein Causes Strand Breaks in Neuronal Genomes in in vitro Models of Parkinson’s Disease. Journal of Alzheimer's disease : JAD, 60(Suppl 1), S133-S150.

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Immunostaining Protocol for Glial Cells

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Cells were grown in 8-well chamber slides (Millipore, Burlington, MA) precoated with poly-d-lysine and laminin to ~50% confluency and fixed with 4% paraformaldehyde and permeabilized with Triton X-100. After blocking with 5% goat serum, cells were incubated with antibodies against GFAP (Molecular Probes, Carlsbad, CA), S100B (Abcam, Cambridge, MA), and Olig2 (Abcam, Cambridge, MA) and subsequently stained with Alexa Fluor 488 or 594 (Molecular Probes, Carlsbad, CA). Images were captured on a laser-scanning confocal microscope (Zeiss model LSM700, San Diego, CA).

Ganguly D., Sims M., Cai C., Fan M, & Pfeffer L.M. (2018). Chromatin Remodeling Factor BRG1 Regulates Stemness and Chemosensitivity of Glioma Initiating Cells. Stem cells (Dayton, Ohio), 36(12), 1804-1815.

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TUNEL Assay for Apoptosis in IFP 1.4 Infection

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To determine whether IFP 1.4 infection impacts on cell apoptosis, cells were plated on 8-well chamber slides (Millipore, Billerica, MA). The terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick-end-labeling (TUNEL) staining for apoptotic nuclei was performed using DEAD End TUNEL kit (Promega, Madison, WI) according to the manufacturer’s instructions with minor modifications. Briefly, cells were fixed in 4% paraformaldehyde for 25 min and then treated with permeabilization solution (0.2% Triton X-100 solution in PBS) for 5 min at room temperature. Labeling reactions were performed with 100 µl of reaction buffer for 60 min at 37°C in a humidified chamber, followed by steptavidin Alexa Fluor 555 conjugate (1∶400, Life Technologies, Carlsbad, CA) staining. Slides were mounted using VECTASHIELD HardSet Mounting Medium with DAPI (Vector Laboratories, Burlingame, CA). Apoptosis was evaluated as the average number of TUNEL-positive cells per DAPI labeled cells at high-power magnification.

Chen L., Phillips M.I., Miao H.L., Zeng R., Qin G., Kim I.M., Weintraub N.L, & Tang Y. (2014). Infrared Fluorescent Protein 1.4 Genetic Labeling Tracks Engrafted Cardiac Progenitor Cells in Mouse Ischemic Hearts. PLoS ONE, 9(10), e107841.

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