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Tcs sp8 x white laser confocal microscope

Manufactured by Leica
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The Leica TCS SP8-X white laser confocal microscope is a versatile and high-performance imaging system designed for advanced biological and materials research. It features a white light laser that provides a wide range of excitation wavelengths, enabling flexible and efficient fluorescence imaging. The microscope is equipped with highly sensitive detectors and advanced optics, allowing for high-resolution, high-contrast imaging of a variety of samples.

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Lab products found in correlation

Vectashield, by Vector Laboratories (4 mentions) Rat monoclonal anti ncadherin, by Developmental Studies Hybridoma Bank (1 mentions) Goat polyclonal anti gfp, by Abcam (1 mentions) Rat monoclonal anti gfp, by BioLegend (1 mentions) Rabbit polyclonal anti dsred, by Takara Bio (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Rabbit polyclonal anti cd4, by Atlas Antibodies (1 mentions) Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions) Anti mouse cy5, by Jackson ImmunoResearch (1 mentions) Anti rabbit cy5, by Jackson ImmunoResearch (1 mentions) Anti myc, by Cell Signaling Technology (1 mentions) Anti rabbit alexa647, by Thermo Fisher Scientific (1 mentions) Anti mouse cy3, by Jackson ImmunoResearch (1 mentions)

4 protocols using tcs sp8 x white laser confocal microscope

1

Immunofluorescent Staining of Drosophila Eyes

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Pupal and adult eye–brain complexes were dissected in cold Schneider’s Drosophila medium and fixed in 4% paraformaldehyde in phosphate-buffered saline for 40 min. Tissues were washed in Phosphate-buffered saline + 0.4% Triton-X (PBST) and mounted in Vectashield (Vector Laboratories, CA). Images were obtained with a Leica TCS SP8-X white laser confocal microscope with a ×63 glycerol objective (c = 1.3). The primary antibodies used in this study with given dilutions were as follows: mouse monoclonal anti-Chaoptin (1:200; Developmental Studies Hybridoma Bank); rat monoclonal anti-nCadherin (1:100; Developmental Studies Hybridoma Bank); rabbit monoclonal anti-Atg8 (1:100; Abcam); mouse monoclonal anti-Trio (1:50; Developmental Studies Hybridoma Bank); mouse monoclonal anti-LAR (1:50; Developmental Studies Hybridoma Bank); goat polyclonal anti-GFP (1:1000; Abcam); rat monoclonal anti-GFP (1:500; BioLegend); rabbit polyclonal anti-CD4 (1:600; Atlas Antibodies); rabbit polyclonal anti-DsRed (1:500; ClonTech); rabbit anti-Syd-1 (1:500; gift from Sigrist Lab). The secondary antibodies Cy3, Cy5 (Jackson ImmunoResearch Laboratories) and Alexa488 (Invitrogen) were used in 1:500 dilution.
Kiral F.R., Linneweber G.A., Mathejczyk T., Georgiev S.V., Wernet M.F., Hassan B.A., von Kleist M, & Hiesinger P.R. (2020). Autophagy-dependent filopodial kinetics restrict synaptic partner choice during Drosophila brain wiring. Nature Communications, 11, 1325.
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2

Visualizing Neuronal Subpopulations in Adult Drosophila Brains

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Adult brains of the following lines LC11-GAL4>UAS-GFP; LC10a-GAL4>UAS-GFP; MB-GAL4>UAS-GFP; CC-GAL4>UAS-GFP; M-DCN-Gal4>UAS-GFP were processed for GFP expression. Brains were dissected in ice-cold PBS, fixed 20 min at room temperature in 4% PFA. Samples were washed three times with PBST (0.3% PBT) for 20 min. Antibody incubations were performed in PBST, normal anti-GFP rabbit serum 1:1000 (Invitrogen, RRID:AB_221569) and anti-nc82 mouse serum 1:100 (Hybridoma Bank, RRID:AB_2314866), overnight at 4°C for primary antibodies and 2h at room temperature for secondary antibodies. After 3 washes in PBST 0.3%, samples were mounted in Vectashield (Vector Laboratories, CA) and imaged using a 20X objective with a Leica TCS SP8-X white laser confocal microscope. Acquired images are visualized and processed offline using Fiji.
Takenaka M., Bagnasco S.M., Preston A.S., Uchida S., Yamauchi A., Kwon H.M, & Handler J.S. (1995). The canine betaine gamma-amino-n-butyric acid transporter gene: diverse mRNA isoforms are regulated by hypertonicity and are expressed in a tissue-specific manner.. Proceedings of the National Academy of Sciences of the United States of America, 92(4), 1072-1076.
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3

Imaging of Eye-Brain Complexes

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Eye-brain complexes were dissected in PBS, fixed in 3.7% paraformaldehyde (PFA) in PBS for 40 minutes, washed in PBST (0.4% Triton-X) and mounted in Vectashield (Vector Laboratories, CA). Images were collected using a Leica TCS SP8-X white laser confocal microscope with a 63X glycerol objective (NA=1.3).
Following antibodies were used for fixed imaging: Primary antibodies: anti-Trio (mouse, 1:50, DSHB), 24B10 (1:200, DSHB), anti-myc (1:200, Cell signaling), anti-Lar (mouse, 1:50, DSHB), anti-Syd-1 (rabbit, 1:500, gift from Stephan Sigrist).
Secondary antibodies: anti-mouse Alexa 647 (1:500, Life technologies), anti-mouse Cy5 (1:500, Jackson laboratories), anti-mouse-Cy3 (1:500, Jackson Laboratories), anti-Rabbit Alexa 647 (1:500, Life Technologies), anti-Rabbit Cy5 (1:500, Jackson Laboratories).
Özel M.N., Kulkarni A., Hasan A., Brummer J., Moldenhauer M., Daumann I.M., Wolfenberg H., Dercksen V.J., Kiral F.R., Weiser M., Prohaska S., von Kleist M, & Hiesinger P.R. (2019). Serial synapse formation through filopodial competition for synaptic seeding factors. Developmental cell, 50(4), 447-461.e8.
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4

Visualizing Neuronal Subpopulations in Adult Drosophila Brains

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Adult brains of the following lines LC11-GAL4>UAS-GFP; LC10a-GAL4>UAS-GFP; MB-GAL4>UAS-GFP; CC-GAL4>UAS-GFP; M-DCN-Gal4>UAS-GFP were processed for GFP expression. Brains were dissected in ice-cold PBS, fixed 20 min at room temperature in 4% PFA. Samples were washed three times with PBST (0.3% PBT) for 20 min. Antibody incubations were performed in PBST, normal anti-GFP rabbit serum 1:1000 (Invitrogen, RRID:AB_221569) and anti-nc82 mouse serum 1:100 (Hybridoma Bank, RRID:AB_2314866), overnight at 4°C for primary antibodies and 2h at room temperature for secondary antibodies. After 3 washes in PBST 0.3%, samples were mounted in Vectashield (Vector Laboratories, CA) and imaged using a 20X objective with a Leica TCS SP8-X white laser confocal microscope. Acquired images are visualized and processed offline using Fiji.
Bengochea M., Sitt J.D., Izard V., Preat T., Cohen L, & Hassan B.A. (2023). Numerical discrimination in Drosophila melanogaster. Cell reports, 42(7), 112772.
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