Dp71 high resolution color digital camera

Embryos were harvested for morphology examination for anomalies. Photos were taken with the Olympus SZX7 microscope mounted with the Olympus DP71 High Resolution Color Digital Camera.
For histology, embryos were fixed in PFA/PBS (4% (w/v) paraformaldehyde (Sigma-Aldrich, Steinheim, Germany) in PBS (phosphate-buffered saline, pH 7.2; Sigma-Aldrich, Steinheim, Germany)) for 18 hours at 4°C, dehydrated in graded series of alcohol (Merck, Darmstadt, Germany), cleared in xylene (RCI Labscan Ltd, Bangkok, Thailand) before being embedded in paraffin (Leica Biosystems, Richmond, IL USA). Sections (8 μm in thickness) were prepared, mounted onto TESPA-coated microscope glass, and stained with hematoxylin and eosin following standard protocol. Photos were taken with the Olympus SZX7 microscope mounted with the Olympus DP71 High Resolution Color Digital Camera.

Skeletons of mouse embryos were stained with alizarin red S and alcian blue following the protocol as published previously [27 (link)]. In brief, embryos were eviscerated and then fixed in 100% ethanol (Merck, Darmstadt, Germany) for 4 days followed with acetone for 3 days. After fixation, embryos were rinsed with water, and then incubated in staining solution containing alizarin red S (0.06%; w/v; Sigma-Aldrich, Steinheim, Germany), alcian blue 8GX (0.02%; w/v; Sigma-Aldrich, Steinheim, Germany), 5% glacial acetic acid in ethanol for 10 days. After staining, the embryos were rinsed with water, then incubated in 20% glycerol (v/v; Sigma-Aldrich, Steinheim, Germany), 1% KOH (w/v; BDH, Poole England) at 37°C for 8 hours, and then incubated in the same solution at room temperature until tissue was completely cleared. Then the solution was replaced with 50% glycerol, 80% glycerol and finally 100% glycerol (USB Corporation, Cleveland, OH USA). Photos were taken with the Olympus SZX7 microscope mounted with the Olympus DP71 High Resolution Color Digital Camera.