Biotin xx goat anti rabbit igg h l

Manufactured by Thermo Fisher Scientific

Biotin-XX Goat Anti-rabbit IgG (H+L) is a secondary antibody conjugated with Biotin. It is designed to detect and bind to rabbit immunoglobulin G (IgG) antibodies, including both heavy and light chains (H+L).

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Lab products found in correlation

Streptavidin horseradish peroxidase, by Vector Laboratories (1 mentions) Donkey anti rabbit igg hrp, by GE Healthcare (1 mentions) Anti actin monoclonal antibody, by Merck Group (1 mentions) Pampkt172, by Cell Signaling Technology (1 mentions) Anti smad3 phospho s423 s425, by Abcam (1 mentions) Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions) Anti pgc 1α, by Abcam (1 mentions) Ampkα, by Cell Signaling Technology (1 mentions) 2 deoxy d glucose 2 dg, by Merck Group (1 mentions)

Spelling variants of this product

Goat anti-Rabbit IgG (H+L) (90 citations) IgG Rabbit (4 citations) H + L IgG (3 citations)

3 protocols using biotin xx goat anti rabbit igg h l

Immunohistochemistry of Testicular Sections

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After deparaffinization and rehydration, the testicular sections were heated for 15 min in a microwave oven at 100°C with sodium citrate buffer (10mM; pH 6),cooled down in the same buffer at RT, then incubated for 20 min with 3% hydrogen peroxide. After washing with phosphate-buffered saline (PBS), the sections were incubated with primary antibodies in a humid chamber at 4°Covernight, then washed again three times in PBS. Primary antibodies were detected by incubation with 1:300Biotin-XX Goat Anti-rabbit IgG (H+L)(Life Technology, B-2770) or Biotin-XX Goat Anti-rat IgG (H+L)(Life Technology,A-10517) for 1 hr at RT, then horseradish peroxidase Streptavidin(SA-5704,Vector) was added. The peroxidase activity was made visible with Vector NovaRED^™ (SK-4800) and counterstained with hematoxylin for 10 sec to 2 min.
Negative controls omitting primary antibodies were included in each experiment. Primary antibodies and dilutions used are presented in Table 1.

Fu C., Begum K., Jordan P.W., He Y, & Overbeek P.A. (2016). Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice. PLoS ONE, 11(8), e0159800.

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Investigating AMPK and Sphingomyelin Signaling

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Cell culture and transfection regents were purchased from Life technologies (Grand Island, NY). p-AMPK^T172 and AMPKα antibodies were purchased from Cell Signaling Technology (Danvers, MA). ASM (H-181), N-SMase2 (H-195), SMS1 (H-130) (for immunohistochemistry), SMS2 (N-13), Smad3 (38-Q), TGFβ1 (V) and rabbit anti-goat IgG-HRP antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). SGMS1 (SMS1) antibody for western blot was purchased from Aviva Systems Biology (San Diego, CA). Anti-PGC1α and anti-Smad3 (phospho S423 + S425) antibodies were purchased from Abcam (San Francisco, CA). Monoclonal anti-actin antibody was purchased from Sigma-Aldrich (St. Louis, MO). Biotin-XX goat anti-rabbit IgG (H + L) were purchased from Life technologies. Donkey anti-rabbit IgG-HRP and sheep anti-mouse IgG-HRP antibodies, and all siRNAs were purchased from GE Healthcare (Chicago, IL, USA). 2-Deoxy-d-glucose (2-DG) was purchased from Sigma-Aldrich.

Miyamoto S., Hsu C.C., Hamm G., Darshi M., Diamond-Stanic M., Declèves A.E., Slater L., Pennathur S., Stauber J., Dorrestein P.C, & Sharma K. (2016). Mass Spectrometry Imaging Reveals Elevated Glomerular ATP/AMP in Diabetes/obesity and Identifies Sphingomyelin as a Possible Mediator. EBioMedicine, 7, 121-134.

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Cell Surface PTHR Immobilization Protocol

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Cell surface PTHR was immobilized by crosslinking specific antibodies using a strategy previously reported^{3 (link)}. Cells were rinsed in buffer containing 150 mM NaCl, 10 mM HEPES, 12.8 mM d-glucose, 2.5 M KCl, 0.5 mM MgCl₂ and 0.5 mM CaCl₂ (pH 8.0) and then incubated at room temperature for 20 min in a 1:200 dilution of polyclonal rabbit anti-GFP antibody (Life Technologies; A-11122). Cells were washed twice and incubated for 20 min at room temperature in a 1:200 dilution of biotin-XX goat anti-rabbit IgG (H + L) (Life Technologies; B2770). Then, cells were washed two times before beginning the experiments.

Jean-Alphonse F.G., Wehbi V.L., Chen J., Noda M., Taboas J.M., Xiao K, & Vilardaga J.P. (2016). β2-adrenergic receptor control of endosomal PTH receptor signaling via Gβγ. Nature chemical biology, 13(3), 259-261.

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