Liposomes were measured for size using DLS (Dynamic Light Scattering) analysis via the 90Plus nanoparticle size analyzer (Brookhaven Instruments Corp.), using 658 nm light observed at a fixed angle of 90º at 20ºC. Liposome samples were diluted with 0.22 μM filter sterilized PBS to a 1.25 nM liposome concentration immediately after extrusion, placed in a 50 μL quartz cuvette and particle sized.
90plus nanoparticle size analyzer
Manufactured by Brookhaven Instruments
Sourced in United States
The 90Plus Nanoparticle Size Analyzer is a laboratory instrument designed to measure the size distribution of nanoparticles. It uses dynamic light scattering technology to determine the hydrodynamic diameter of particles in the range of 0.3 to 10,000 nanometers.
Automatically generated - may contain errors
Lab products found in correlation
Spectrum 100, by PerkinElmer (1 mentions)
Bx53 fluorescence microscope, by Olympus (1 mentions)
X cite series 120 q, by Olympus (1 mentions)
Tecnai t12, by Thermo Fisher Scientific (1 mentions)
S 4800, by Thermo Fisher Scientific (1 mentions)
Dp72 cooled ccd camera, by Olympus (1 mentions)
Leo 906, by Zeiss (1 mentions)
90 plus particle size analyzer, by Brookhaven Instruments (1 mentions)
V 670 spectrophotometer, by Jasco (1 mentions)
1200 series system, by Agilent Technologies (1 mentions)
Jem 1400, by JEOL (1 mentions)
Spectramax i3x, by Molecular Devices (1 mentions)
Uv 2550, by Shimadzu (1 mentions)
Fiveeasy plus, by Mettler Toledo (1 mentions)
10 protocols using 90plus nanoparticle size analyzer
1
Dynamic Light Scattering of Liposomes
2
Particle Size Distribution of Plant PIH
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The particle size distribution (PSD) of plant PIH was determined using a 90Plus Nanoparticle Size Analyzer (Brookhaven Instruments, Holtsville, NY, US). The particle sizes of the samples were analyzed using the method proposed by Win and Feng [28 (link)]. A sample solution (0.5% w/v) was prepared by dissolving the required amount of sample in secondary water (dd water), and stirred at 25 °C for 20 min. Then, the sample solution was diluted 10 times with secondary water and centrifuged at 12,000× g for 20 min at 25 °C. The supernatant (3 mL) was injected into a cuvette to measure particle size. Specifically, the laser light hits the particle and generates scattered light, and the detector measures the intensity change of the scattered light to calculate the particle size. The signal range is 1 nm–10 µm. All measurements were repeated three times (n = 3).
3
Biophysical Characterization of Protein Solutions
4
Particle Size Analysis of PPI and PPIH
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Particle size distributions (PSDs) of PPI and PPIH were obtained on the basis of the method of Win and Feng [42 (link)] using a 90Plus Nanoparticle Size Analyzer (Brookhaven Instruments, Holtsville, New York, NY, USA). PPI and PPIH solutions (0.05% w/v) were prepared using the required number of samples solubilized in dd water and stirred for 20 min at 25 °C. The PPI and PPIH solutions were centrifuged at 12,000× g for 20 min at 25 °C. The supernatants of the PPI and PPIH solutions (3 mL) were loaded into a cuvette to measure particle size. By using the principles of dynamic light scattering, the PSD was obtained from the velocity distribution of particles suspended in a dispersing medium. The signal was analyzed with sizes ranging from 1 nm to 10 µm. Each sample was analyzed in triplicate.
5
Characterization of Functionalized Gold Nanorods
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Characteristic surface plasmon resonances of GNRs were
recorded in the wavelength region of 400 to 900 nm, using a
Perkin Elmer spectrophotometer (Lambda 25). For Fourier-
transform infrared spectroscopy (FTIR) analysis, samples
of bare GNRs, silica, and folic acid modified GNRs were
made into a dry powder by a lyophilizer (LYSFME-Snijders
scientific). Spectra were recorded on a NICOLET IR 100
(FT-IR) and reported in the range of 500-3,800 cm-1.
For transmission electron microscopy (TEM)
characterization, purified and surface modified GNRs
were deposited on carbon-coated copper grids and imaged
utilizing TEM (LEO 906, Zeiss).
The dynamic light scattering (DLS) was performed
by Brookhaven 90Plus Nanoparticle Size Analyzer to
identify the effective diameter and size distribution of
GNRs. The surface charge of F-Si-GNR was measured
with Zeta potential measurements in water (NICOMP
380ZLS Zeta potential/Particle sizer).
recorded in the wavelength region of 400 to 900 nm, using a
Perkin Elmer spectrophotometer (Lambda 25). For Fourier-
transform infrared spectroscopy (FTIR) analysis, samples
of bare GNRs, silica, and folic acid modified GNRs were
made into a dry powder by a lyophilizer (LYSFME-Snijders
scientific). Spectra were recorded on a NICOLET IR 100
(FT-IR) and reported in the range of 500-3,800 cm-1.
For transmission electron microscopy (TEM)
characterization, purified and surface modified GNRs
were deposited on carbon-coated copper grids and imaged
utilizing TEM (LEO 906, Zeiss).
The dynamic light scattering (DLS) was performed
by Brookhaven 90Plus Nanoparticle Size Analyzer to
identify the effective diameter and size distribution of
GNRs. The surface charge of F-Si-GNR was measured
with Zeta potential measurements in water (NICOMP
380ZLS Zeta potential/Particle sizer).
6
Characterizing Nanoparticle Size and Zeta Potential
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Photon correlation spectroscopy (PCS), also known as dynamic light scattering (DLS), was used to determine the average hydrodynamic diameter (HDD) and polydispersity index (PDI) of each batch of nanoparticles (90 Plus Nanoparticle Size Analyzer; 35 mV red diode laser source, ʎ = 640 nm; Brookhaven Instruments Corporations, Holtsville, NY, USA). Each suspension sample was diluted with DDW to yield an appropriate scattering intensity of 100–400 kcps. All measurements were performed three times at 25 °C with angle detection fixed at 90° on 2 mL samples.
The zeta potential (ZP) of the synthesized nanoparticles was determined by electrophoretic laser Doppler anemometry with the 90 Plus Particle Size Analyzer (Brookhaven Instruments Corporation). Suspension samples were prepared as described before and analyzed in triplicate. All results are shown as the mean and standard deviation (s.d.) of the values obtained from three different batches.
The zeta potential (ZP) of the synthesized nanoparticles was determined by electrophoretic laser Doppler anemometry with the 90 Plus Particle Size Analyzer (Brookhaven Instruments Corporation). Suspension samples were prepared as described before and analyzed in triplicate. All results are shown as the mean and standard deviation (s.d.) of the values obtained from three different batches.
7
Characterizing Gold Nanoparticles: TEM and UV-Vis
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For TEM analysis,
5 μL of the AuNP dispersions was deposited onto a copper grid
coated with a carbon membrane and examined using a microscope operating
at 120 kV. The TEM was equipped with a CCD camera. The size (min Feret
diameter and its standard deviation), as well as the shape parameters
(aspect ratio, projection area, and perimeter) of the AuNPs were determined
using an open-source image processing program called C6H6.42 To record the UV–vis
extinction spectrum, a Jasco V-670 spectrophotometer was used with
10 mm path-length quartz Suprasil-grade cuvettes at 25 °C. Before
measurement, all dispersions were diluted 20-fold in Milli-Q water.
DLS measurements of the 5-fold diluted AuNPs in Milli-Q water at
25 °C were performed using a 90 Plus Nanoparticle Size Analyzer
(Brookhaven) and reusable plastic cuvettes.
5 μL of the AuNP dispersions was deposited onto a copper grid
coated with a carbon membrane and examined using a microscope operating
at 120 kV. The TEM was equipped with a CCD camera. The size (min Feret
diameter and its standard deviation), as well as the shape parameters
(aspect ratio, projection area, and perimeter) of the AuNPs were determined
using an open-source image processing program called C6H6.42 To record the UV–vis
extinction spectrum, a Jasco V-670 spectrophotometer was used with
10 mm path-length quartz Suprasil-grade cuvettes at 25 °C. Before
measurement, all dispersions were diluted 20-fold in Milli-Q water.
DLS measurements of the 5-fold diluted AuNPs in Milli-Q water at
25 °C were performed using a 90 Plus Nanoparticle Size Analyzer
(Brookhaven) and reusable plastic cuvettes.
8
Nanoparticle Characterization by DLS and HPLC
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Particle sizes were measured using DLS analysis via the 90Plus Nanoparticle Size Analyzer (Brookhaven Instruments Corp., Long Island, NY), using 658 nm light observed at a fixed angle of 90° at 20 °C. Confirmation of the components of the nanoparticle formulations was determined by RP-HPLC on an Agilent (Santa Clara, CA) 1200 series system with a Zorbax C3 column with an IPA gradient in the mobile phase. The column was monitored with a diode array detector from 200 to 400 nm wavelengths. Extruded nanoparticles were compared with equivalent samples of the base components to confirm that the resulting formulations were composed of intended ratios of the individual lipids and conjugates and that the stoichiometries that were used for synthesis of the particles were conserved in the final product.
9
Morphological Characterization of Nanoparticles
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The morphology of PLGA, PLGA-chitosan, and PLGA-lovastatin-chitosan-tetracycline nanoparticles was examined by transmission electron microscopy (JEM-1400, JEOL Ltd., Tokyo, Japan) at an accelerating voltage of 80 kV. Nanoparticles treated with 1% uranyl acetate on copper grids were used for negative staining. Particle size was measured via dynamic light scattering analyses using a 90Plus Nanoparticle Size Analyzer (Brookhaven Instruments Corporation, Holtsville, NY, USA). The particle size measurements were conducted at a fixed 90° angle, a wavelength of 632.8 nm, and temperature of 25°C. For all measurements, specimens were equilibrated in a temperature-controlled chamber for 5 minutes and then dynamic light scattering measurements were promptly started. Data collection and analysis were performed using BIC software (Brookhaven Instruments Corporation) with a dust filter cutoff value setting at 30.
10
Characterization of Polymer Dots
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The particle size and zeta potential of the polymer dots were measured in aqueous solution using a Dynamic Light Scattering (DLS) instrument (Brookhaven 90 Plus Nanoparticle Size Analyzer). The absorption spectrum (360–750 nm) was obtained with a Shimadzu UV-2550 ultraviolet-visible spectrometer. The fluorescence spectrum (562–800 nm) was measured with an excitation wavelength at 537 nm with SpectraMax i3x (MOLECULAR DEVICES). pH was measured by FiveEasy Plus (METTLER TOLEDO). The fluorescence quantum yield (QY) of the polymer dots was measured with a UV-NIR absolute PL QY spectrometer (Hamamatsu, Japan) with 510 nm excitation for polymer dots from a xenon lamp.42 (link) In the size and fluorescence stability test, polymer dots were dispersed in DMEM supplemented with 10% FBS at 37 °C for 96 h.
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