Fatty acids methyl ester reference standard mixture 37 standard 47885 u

Acetonitrile 99.9%, n-hexane 95% and methanol 99.9% were purchased from Fisher Scientific (Shanghai, China). The fatty acids methyl ester (FAME) reference standard mixture 37 (standard 47885-U) was purchased from Sigma (St. Louis, MO, USA). Sugars standards [glucose, sorbitol and d(+)-trehalose and myo-inositol], phenolic standards (gallic, 4-hydroxybenzoic, 3,4-dihydroxybenzaldehyde, homogentisic, p-coumaric and o-coumaric acids) were purchased from Sigma (St. Louis, MO, USA) or Sigma-Aldrich Chem (shanghai, China). 1,1-Diphenyl-2-picryl-hydrazyl (DPPH), BHT, 3-(2-pyridyl)-5,6-bis (4-phenyl-sulfonic acid)-1,2,4-triazine (ferrozine), rutin and ascorbic acid were purchased from Sigma Chemical Co. BSTFA + TMCS (99:1) was purchased from Supelco. Folin–Ciocalteu’s phenol reagent was purchased from Beijing Solarbio reagent company (Beijing, China). Ferrous chloride, sodium salicylate, ferrous sulphate, hydrogen peroxide, potassium ferricyanide, trichloroacetic acid, disodium phosphate, sodium dihydrogen phosphate, ferric chloride, sodium carbonate, sodium hydroxide, aluminium nitrate, sodium nitrite, petroleum ether (60–90 °C), ethanol and methanol were all purchased from Beijing chemical plant (Beijing, China). All chemicals were of analytical grade. Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, USA).

Acetonitrile 99.9%, n-hexane 95% and ethyl acetate 99.8% were of HPLC grade from Fisher Scientific (Lisbon, Portugal). The fatty acids methyl ester (FAME) reference standard mixture 37 (standard 47885-U) was purchased from Sigma (St. Louis, MO, USA), as also other individual fatty acids, trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), sugar, tocopherol, organic acid and phenolic compound standards. Racemic tocol, 50 mg/mL, was purchased from Matreya (Pleasant Gap, PA, USA). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was obtained from Alfa Aesar (Ward Hill, MA, USA). Fetal bovine serum (FBS), Hank’s balanced salt solution, nonessential amino acids solution (2 mM), penicillin/streptomycin solution (100 U/mL and 100 µg/mL, respectively), DMEM medium was from Hyclone (Logan, Utah, USA). Acetic acid, ellipticine and sulforhodamine B (SRB) were from Sigma Chemical Co. Methanol and all other chemicals were of analytical grade and purchased from common sources. Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Topway Global, Greenville, SC, USA).

Acetonitrile 99.9%, n-hexane 95%, and ethyl acetate 99.8% of HPLC grade were obtained from Lab-Scan (Lisbon, Portugal). Methanol and all other chemicals were of analytical grade and were obtained from common sources.
The fatty acids methyl ester (FAME) reference standard mixture 37 (standard 47885-U) was purchased from Sigma-Aldrich (St. Louis, MO, USA), as also were sugar standards, organic acids, and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox). Tocol and tocopherol standards were purchased from Matreya (Pleasant Gap, PA, USA).
2,2-Diphenyl-1-picrylhydrazyl was obtained from Alfa Aesar (Ward Hill, MA, USA). p-iodonitrotetrazolic chloride (INT) dye was purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as an indicator of microbial growth. Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, CA, USA).