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Mm nf mabf515

Manufactured by Merck Group
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The MM_NF-MABF515 is a laboratory equipment product manufactured by Merck Group. It is designed to perform nanofiltration of monoclonal antibodies. The core function of this product is to separate and purify monoclonal antibodies from complex mixtures using a nanofiltration membrane technology.

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Lab products found in correlation

At8 anti p tau, by Thermo Fisher Scientific (2 mentions) Chemidoc imaging system, by Bio-Rad (2 mentions) Nitrocellulose membrane, by Bio-Rad (2 mentions) Anti tuj1, by Abcam (2 mentions) P tau phf 1, by Abcam (2 mentions) Cy5 anti mouse secondary antibody, by Jackson ImmunoResearch (2 mentions) Anti map2, by Cell Signaling Technology (2 mentions) Anti aldh1l1, by Merck Group (2 mentions) Bis tris gradient gel, by Thermo Fisher Scientific (2 mentions)

2 protocols using mm nf mabf515

1

Western Blot Analysis of Neural Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates (15–75 μg) were resolved on 12% Bis-Tris or 4–12% gradient Bis/Tris gels (Life Technologies) and the proteins were transferred to nitrocellulose membranes (Bio-Rad). For amyloid-ß western blot analysis, the membranes were cross-linked with 0.5% glutaraldehyde solution before blocking. Western blot results were visualized using enhanced chemiluminescence (ECL). Signals were captured using ChemiDoc imaging system (Bio-Rad) and quantified using ImageJ software (NIH). The following primary antibodies were used (dilutions): anti- Aβ (1:100, 6e10; Signet); anti-Tuj1 (1:200, Abcam, ab24629); anti-MAP2 (1:200, Cell Signaling, 4542); anti-GFAP (1:100, Neuromap, N206A/8); anti-p-tau AT8 (1:30, Thermo Scientific, MN1020), p-tau PHF-1 (1:200, Abcam, ab66275), CD68 (1:200, BD Bioscience, 556059), CD11b (1:200, EMD Millipore, MM_NF-MABF515), anti-ALDH1L1 (1:200, EMD Millipore), Cy5 anti-mouse secondary antibody (1:400, Jackson Immunoresearch, 715-175-150).
Park J., Wetzel I., Marriott I., Dréau D., D’Avanzo C., Kim D.Y., Tanzi R.E, & Cho H. (2018). A 3D Human Tri-Culture System Modeling Neurodegeneration and Neuroinflammation in Alzheimer’s Disease. Nature neuroscience, 21(7), 941-951.
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2

Western Blot Analysis of Neural Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates (15–75 μg) were resolved on 12% Bis-Tris or 4–12% gradient Bis/Tris gels (Life Technologies) and the proteins were transferred to nitrocellulose membranes (Bio-Rad). For amyloid-ß western blot analysis, the membranes were cross-linked with 0.5% glutaraldehyde solution before blocking. Western blot results were visualized using enhanced chemiluminescence (ECL). Signals were captured using ChemiDoc imaging system (Bio-Rad) and quantified using ImageJ software (NIH). The following primary antibodies were used (dilutions): anti- Aβ (1:100, 6e10; Signet); anti-Tuj1 (1:200, Abcam, ab24629); anti-MAP2 (1:200, Cell Signaling, 4542); anti-GFAP (1:100, Neuromap, N206A/8); anti-p-tau AT8 (1:30, Thermo Scientific, MN1020), p-tau PHF-1 (1:200, Abcam, ab66275), CD68 (1:200, BD Bioscience, 556059), CD11b (1:200, EMD Millipore, MM_NF-MABF515), anti-ALDH1L1 (1:200, EMD Millipore), Cy5 anti-mouse secondary antibody (1:400, Jackson Immunoresearch, 715-175-150).
Park J., Wetzel I., Marriott I., Dréau D., D’Avanzo C., Kim D.Y., Tanzi R.E, & Cho H. (2018). A 3D Human Tri-Culture System Modeling Neurodegeneration and Neuroinflammation in Alzheimer’s Disease. Nature neuroscience, 21(7), 941-951.
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