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Mx3005tmp real time detection system

Manufactured by Agilent Technologies
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Sourced in United States

The Mx3005P Real-time detection system is a product offered by Agilent Technologies. It is a lab equipment designed for real-time detection applications. The core function of this system is to facilitate real-time detection of target samples.

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Lab products found in correlation

Sybr premix ex taqtm gc, by Takara Bio (1 mentions) Sybr premix ex taqtm, by Takara Bio (1 mentions)

Close spelling variants of this product

REAL Detection System (12 citations) Real-time System (2 citations)

2 protocols using mx3005tmp real time detection system

1

Comparative RT-qPCR Analysis of mazEF Mutant

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Total RNA of both wild type and mazEF mutant strains were extracted as described (Dai et al., 2020 (link)) and then cDNA was amplified using SYBR Premix Ex TaqTM GC (TaKaRa). RT-qPCR assays were performed using the Mx3005TMP Real-time detection system (Agilent, United States) and groEL was used to normalize RNA input. Experiments were repeated three times. RT-qPCR primers are listed in Supplementary Table S1.
Dai J., Chen Z., Hou J., Wang Y., Guo M., Cao J., Wang L., Xu H., Tian B, & Zhao Y. (2021). MazEF Toxin-Antitoxin System-Mediated DNA Damage Stress Response in Deinococcus radiodurans. Frontiers in Genetics, 12, 632423.
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2

Validating RNA-seq Data Quality via RT-qPCR

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The reliability of the RNA-seq data was verified using 13 DEGs with different fold-change values (seven up-regulated and six down-regulated genes). Total RNA was extracted and cDNA was amplified using SYBR Premix Ex TaqTM (TaKaRa). RT-qPCR assays were performed using the Mx3005TMP Real-time detection system (Agilent, United States) and DR_1343 was used to normalize expression. RT-qPCR primers are listed in Supplementary Table S2.
Li S., Cai J., Lu H., Mao S., Dai S., Hu J., Wang L., Hua X., Xu H., Tian B., Zhao Y, & Hua Y. (2019). N4-Cytosine DNA Methylation Is Involved in the Maintenance of Genomic Stability in Deinococcus radiodurans. Frontiers in Microbiology, 10, 1905.
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