Mmv00

Manufactured by R&D Systems

Sourced in United States

The MMV00 is a laboratory equipment device designed for research and development purposes. It serves as a tool for specific applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation on its intended use.

Automatically generated - may contain errors

Lab products found in correlation

Ripa buffer, by Thermo Fisher Scientific (4 mentions) M6250, by Merck Group (2 mentions) P8783, by Merck Group (2 mentions) H 2270, by Merck Group (2 mentions) P 7505, by Merck Group (2 mentions) Gw9508, by Cayman Chemical (2 mentions) Mlb00c, by R&D Systems (2 mentions) Mcx120, by R&D Systems (2 mentions) Mbb00, by R&D Systems (2 mentions) Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Pierce bca protein assay, by Thermo Fisher Scientific (2 mentions) Spectramax plus, by Molecular Devices (2 mentions) Ab654, by Abcam (2 mentions) Ab113474, by Abcam (2 mentions) Nb300 666, by Abcam (2 mentions) Ab652, by Merck Group (2 mentions) Ab1344, by Abcam (2 mentions) Nb100 134, by Novus Biologicals (2 mentions) Fibrillarin, by Cell Signaling Technology (2 mentions) Na934v, by Cytiva (2 mentions)

Close spelling variants of this product

Quantikine (MMV00) (3 citations)

28 protocols using mmv00

Measuring Mouse VEGF Levels

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Mouse vascular endothelial growth factor (Vegf) ELISA kit was purchased from R&D Systems (Quantikine, MMV00) and used according to manufacturer’s instructions.

Serrano-Pozo A., Sánchez-García M.A., Heras-Garvín A., March-Díaz R., Navarro V., Vizuete M., López-Barneo J., Vitorica J, & Pascual A. (2017). Acute and Chronic Sustained Hypoxia Do Not Substantially Regulate Amyloid-β Peptide Generation In Vivo. PLoS ONE, 12(1), e0170345.

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Cone Photoreceptor Cell Culture and Hypoxia Signaling

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Cone photoreceptor cells^{47 (link),48 (link)} (661W; from Dr. Al-Ubaidi) were cultured as monolayers at 37 °C, 5% CO₂ in a humidified atmosphere in DMEM with FBS 10% supplemented with hydrocortisone (20 µg/500 mL, H-2270, Sigma), Progesterone (20 µg/500 mL, P-8783, Sigma), Putrescine (0.016 g/500 mL, P-7505, Sigma) and β-mercaptoethanol (20 µL/500 mL, M-6250, Sigma). Cells were not contaminated by mycoplasma. Equal number of 661W cells (0.3×10⁶) were plated in 6-well dishes and cultured to 80% confluence. Cells were washed twice with PBS, starved for 4 hours (above medium without FBS) then stimulated with GW9508 (14 µM, Cayman) or vehicle. Photoreceptors were then collected 8 hours post-treatment for Hif1a protein expression (see western blot); while their medium was collected at 12 hours for Vegfa quantification by ELISA (as per manual, MMV00, R&D Systems). Vegfa concentration was normalized for the number of cells per well, by doing a Bradford to measure the total cell protein content of each well.

Joyal J.S., Sun Y., Gantner M.L., Shao Z., Evans L.P., Saba N., Fredrick T., Burnim S., Kim J.S., Patel G., Juan A.M., Hurst C.G., Hatton C.J., Cui Z., Pierce K.A., Bherer P., Aguilar E., Powner M.B., Vevis K., Boisvert M., Fu Z., Levy E., Fruttiger M., Packard A., Rezende F.A., Maranda B., Sapieha P., Chen J., Friedlander M., Clish C.B, & Smith L.E. (2016). Retinal lipid and glucose metabolism dictates angiogenesis through lipid sensor Ffar1. Nature medicine, 22(4), 439-445.

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Quantifying VEGF and cAMP Levels

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VEGF and cAMP levels were determined using a sensitive ELISA kit according to the manufacturer's instruction (MMV00 and KGE002B, R&D Systems Inc.).

Seki T., Hosaka K., Lim S., Fischer C., Honek J., Yang Y., Andersson P., Nakamura M., Näslund E., Ylä-Herttuala S., Sun M., Iwamoto H., Li X., Liu Y., Samani N.J, & Cao Y. (2016). Endothelial PDGF-CC regulates angiogenesis-dependent thermogenesis in beige fat. Nature Communications, 7, 12152.

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Quantification of Inflammatory Biomarkers in Endometriosis

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Interleukin 1β (IL-1β) (MLB00C, R&D, Minneapolis, MN, USA), tumor necrosis factor-α (TNF-α) (430907, Biolegend, San Diego, CA, USA), vascular endothelial growth factor (VEGF) (MMV00, R&D, Minneapolis, MN, USA) in the blood serum, and VEGF in endometriosis lesions were quantified by enzyme-linked immunosorbent assay (ELISA) kit following the manufacturer’s protocol.

Chang L.C., Chiang Y.F., Chen H.Y., Huang Y.J., Liu A.C, & Hsia S.M. (2020). The Potential Effect of Fucoidan on Inhibiting Epithelial-to-Mesenchymal Transition, Proliferation, and Increase in Apoptosis for Endometriosis Treatment: In Vivo and In Vitro Study. Biomedicines, 8(11), 528.

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Quantifying Mouse Plasma VEGF Levels

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ELISA was used for determining mouse VEGF protein concentrations. To detect circulating protein levels, mouse plasma VEGF (MMV00, R&D Systems) were determined according to the manufacturer’s protocol with the standard curve. Absorbance values were detected at 450 nm using a microplate reader and values were calculated using the formula obtained from the trendline.

Jing X., Wu J., Dong C., Gao J., Seki T., Kim C., Urgard E., Hosaka K., Yang Y., Long S., Huang P., Zheng J., Szekely L., Zhang Y., Tao W., Coquet J., Ge M., Chen Y., Adner M, & Cao Y. (2022). COVID-19 instigates adipose browning and atrophy through VEGF in small mammals. Nature Metabolism, 4(12), 1674-1683.

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Serum ANGPTL2 and VEGF Quantification

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Serum ANGPTL2 (1F-716, Immuno-biological laboratories Co., Ltd., Japan) and VEGF (MMV00, R&D Systems, Minneapolis, MN, USA) levels were measured by using a commercially available human ELISA kits according to the manufacturers’ instructions, respectively.

Wang Y., Zheng Z., Yang Y., Lang J., Zhang N., Yang L, & Zhao D. (2020). Angiopoietin-like 2 is a potential biomarker for diabetic foot patients. BMC Endocrine Disorders, 20, 178.

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Comprehensive Mouse Plasma Proteome Analysis

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Mouse plasma protein concentration was measured for PlGF2 (dilution factor: 1), VEGF (dilution factor: 2), SDF1α (dilution factor: 1), G-CSF (dilution factor: 1), HGF (dilution factor: 1), TGFβ1 (dilution factor: 60), OPN (dilution factor: 100), sVEGFR2 (dilution factor: 20), CSF (dilution factor: 2), and Leptin (dilution factor: 10). All ELISA kits were purchased from R&D Systems (catalogue numbers: MP200, MMV00, MCX120, MCS00, DY2207, MB100B, MOST00, MVR200, MCK00, MOB00, respectively) and used according to manufacturer’s instructions. Protein levels after treatment were normalized to corresponding vehicle controls, and fold changes in log2 scale were graphed.

Mastri M., Lee C.R., Tracz A., Kerbel R.S., Dolan M., Shi Y, & Ebos J.M. (2018). Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors. Molecular cancer therapeutics, 17(7), 1602-1612.

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Quantification of VEGF and IGFBP Secretion in Stem Cell Cultures

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The media of MESCs and HESCs were collected at different time points, as described above, from at least 3 wells of a 6-well plate and were stored at -80°C. Media samples were subjected to enzyme-linked immunosorbent assays (ELISA) and the data were analyzed according to the manufacturer's instructions. All samples were measured in duplicates and the total protein content of the culture medium was calculated. Some samples were diluted to match the dynamic range of each ELISA kit. Mean protein production was normalized to the cell counts of each sample assayed. Protein estimates were obtained from at least two independent samples for HESC and at least three independent samples for MESC. The data are reported as mean protein per cell ± S.E.M.
The analytical sensitivities of each kit were: 3 pg/mL for the mouse VEGF ELISA kit (MMV00, R&D Systems), 6.4 pg/mL for the mouse IGFBP4 ELISA kit (SEA055Mu, Uscn Life Science Inc.), 9 pg/mL for the human VEGF ELISA kit (DDV00, R&D Systems), < 5 pg/mL for the human IGFBP1 ELISA kit (ab100539, Abcam).

Davila J., Laws M.J., Kannan A., Li Q., Taylor R.N., Bagchi M.K, & Bagchi I.C. (2015). Rac1 Regulates Endometrial Secretory Function to Control Placental Development. PLoS Genetics, 11(8), e1005458.

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Quantifying VEGF in Grafted DMS and MNC

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To determine the neovascularization within the engrafted DMS and MNC post-intrperitoneal transplantation, quantification of vascular endothelial growth factor (VEGF) was performed using Mouse VEGF Immunoassay kit (MMV00, R&D System) as per the manufacturer's instructions. Lysates prepared from each implant along with surrounding host tissues were used for VEGF estimation and compared with the control. The amount of VEGF was expressed in pg/mL of the lysate used for estimation. Five samples of each group were used for VEGF estimation in three different experiments to enhance the reproducibility of the analysis.

Vishwakarma S.K., Bardia A., Lakkireddy C., Paspala S.A, & Khan A.A. (2018). Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury. Frontiers in Bioengineering and Biotechnology, 6, 150.

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Quantitative Tissue Biomarker Assay

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Harvested lung and kidney tissues were snap-frozen in liquid nitrogen
and homogenized in RIPA lysis buffer (27 (link)). Blood samples obtained via terminal cardiac punctures were
collected in heparin-coated plasma-separating tubes (BD Microtainer #365985) and
centrifuged for 10 minutes at 1,000 RCF (g) to isolate plasma.
Quantikine ELISA kits were used to measure murine Ang2 (R&D #MANG20),
VEGF (#MMV00), PDGF-BB (#MBB00), and erythropoietin (EPO; #MEP00) levels in
plasma or tissue homogenates.

Wu F.T., Man S., Xu P., Chow A., Paez-Ribes M., Lee C.R., Pirie-Shepherd S.R., Emmenegger U, & Kerbel R.S. (2016). Efficacy of Cotargeting Angiopoietin-2 and the VEGF Pathway in the Adjuvant Postsurgical Setting for Early Breast, Colorectal, and Renal Cancers. Cancer research, 76(23), 6988-7000.

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