Bax 6a7 antibody

Manufactured by Santa Cruz Biotechnology

Sourced in Japan

The Bax 6A7 antibody is a specific monoclonal antibody produced by Santa Cruz Biotechnology. It is designed to recognize and bind to the Bax protein, a key regulator of apoptosis (programmed cell death). The Bax 6A7 antibody can be used in various research applications to study the role of Bax in cellular processes.

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Lab products found in correlation

Bax n20 antibody, by Santa Cruz Biotechnology (2 mentions) Protein g mag sepharose, by GE Healthcare (1 mentions) Protein a g bead, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Bax antibody (18 citations) Bax (6A7) (6 citations)

2 protocols using bax 6a7 antibody

Bax Protein Immunoprecipitation from A549 Cells and Lung Tissue

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Homogenized A549 cells and mice lung tissues were incubated with Bax 6A7 antibody (Santa Cruz Biotechnology) for 6 h and mixed using rotary mixer NRC-200 (NISSEN, Tokyo, Japan) at 4°C temperature. Next, samples were incubated with protein G Mag Sepharose (GE Healthcare UK Ltd, Little Chalfont, Buckinghamshire, UK) for 6 h again. The antibody/antigen complex was pulled out of the sample using magnetic interaction between protein G Mag and Magrock6 (GE Healthcare UK Ltd). This isolated protein was separated by SDS-PAGE for western blot analysis. The Bax protein was detected using Bax N-20 antibody (Santa Cruz Biotechnology) after electrophoresis and transfer.

Suzuki K., Yanagihara T., Yokoyama T., Maeyama T., Ogata-Suetsugu S., Arimura-Omori M., Mikumo H., Hamada N., Harada E., Kuwano K., Harada T, & Nakanishi Y. (2017). Bax-inhibiting peptide attenuates bleomycin-induced lung injury in mice. Biology Open, 6(12), 1869-1875.

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Regulation of BAX Activation by BCL-2 BH4 Peptides

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Recombinant BAX (1 μM) was mixed with 8 μl liposomes,1 μM BIM SAHB_A (aa 145–164), and the indicated doses of BCL-2 BH4 SAHB_A or BCL-2 BH4 SAHB_A L23A in 20 μl liposomal buffer (10 mM HEPES [pH 7.0], 200 mM KCl, 1 mM MgCl₂). Following the indicated incubation period at room temperature, the mixture was added to 280 μl 3% BSA in PBS, and 30 μl of the resulting mixture (10%) was reserved for input analysis. Activated BAX was immunoprecipitated with BAX 6A7 antibody (Santa Cruz Biotechnology) conjugated to pre-washed protein A/G beads (Thermo Scientific) or beads alone, and resolved by electrophoresis. Immunoblotting of the inputs and immunoprecipitated samples was performed with the BAX N20 antibody (Santa Cruz).

Barclay L.A., Wales T.E., Garner T.P., Wachter F., Lee S., Guerra R.M., Stewart M.L., Braun C.R., Bird G.H., Gavathiotis E., Engen J.R, & Walensky L.D. (2015). Inhibition of Pro-Apoptotic BAX by a Noncanonical Interaction Mechanism. Molecular cell, 57(5), 873-886.

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