Normal human airway epithelial cell line, BEAS-2B, normal human embryonic liver cell line, LO2, and human embryonic kidney cell line, HEK293, were supplied by American Type Culture Collection (Rockville, MD). Cells were cultured in Gibco DMEM (Cat: 12100046) supplemented with 10% Gibco fetal bovine serum and 1% Gibco penicillin-streptomycin-glutamine (Thermo Fisher Scientific, Waltham, USA). Cells were cultured at 37 °C in a humidified incubator containing 5% CO2.
Gibco penicillin streptomycin glutamine
Manufactured by Thermo Fisher Scientific
Sourced in United States
Gibco penicillin-streptomycin-glutamine is a sterile, liquid cell culture supplement that contains three essential components: penicillin, streptomycin, and L-glutamine. It is used to support the growth and health of cultured cells in various research applications.
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Lab products found in correlation
Gibco fetal bovine serum, by Thermo Fisher Scientific (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Gibco dmem, by Thermo Fisher Scientific (1 mentions)
Beas 2b, by American Type Culture Collection (1 mentions)
Hek293t cell, by American Type Culture Collection (1 mentions)
Raw264.7 cell, by American Type Culture Collection (1 mentions)
Vero e6, by American Type Culture Collection (1 mentions)
Hek 293t ace2h cells, by Sino Biological (1 mentions)
2 protocols using gibco penicillin streptomycin glutamine
1
Cell Culture of Common Cell Lines
2
SARS-CoV-2 Infection Experiments in Cell Lines
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HEK-293T cells, RAW264.7 cells, and the African green monkey epithelial (Vero E6) cells were supplied by American Type Culture Collection (ATCC, Rockville, MD, United States). HEK-293T-ACE2h cells were constructed by Sino Biological (Beijing, China). All the cell lines were cultured in Dulbecco Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum and 1% Gibco penicillin-streptomycin-glutamine (Thermo Fisher Scientific, Waltham, United States) at 37°C in a humidified atmosphere of 5% CO2. Besides, 100 μg/mL hygromycin was used for the culture of HEK-293T-ACE2h cells. SARS-CoV-2 (Genebank accession no. MT123290.1 ) was clinical isolate from the First Affiliated Hospital of Guangzhou Medical University. The viral titer was determined by 50% tissue culture infective dose (TCID50) according to the cytopathic effect by use of Reed-Muench method. All the infection experiments of SARS-CoV-2 strain were performed in a biosafety level-3 laboratory.
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