GFP-tagged hWJ-MSCs (HUXUC-01101, passage #5) were purchased from Cyagen Biosciences Inc. (Guangzhou, China) and cultured in hWJ-MSCs complete medium (HUXUC-90011, Cyagen Biosciences Inc.) supplemented with 10% human UCMSCs specific FBS, 100 mg/mL streptomycin and 100 U/mL penicillin, and incubated at 37°C in a 5% CO2 humidified incubator. After 72 hours, non-adherent cells were removed, and fresh medium was added; the medium was changed every 3 days. When adherent cells were 90% confluent, they were trypsinized (trypsin-EDTA solution; HUXUB-90011, Cyagen Biosciences Inc.) and seeded onto fresh plates (split 1: 3) until a homogenous population was obtained after 2 to 3 weeks of culture. hWJ-MSCs at passage 8–9 were used in all experiments.
Huxuc 90011
Manufactured by Cyagen
Sourced in China
The HUXUC-90011 is a laboratory equipment designed for general use in research and scientific applications. The core function of this product is to provide a controlled environment for various experimental procedures. Detailed specifications and intended use are not available.
Automatically generated - may contain errors
3 protocols using huxuc 90011
1
Culturing GFP-tagged hWJ-MSCs for Research
2
Characterizing Multipotent Mesenchymal Stem Cells
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MSCs derived from different tissues (BM, AT, and UC) were obtained from Cyagen Biosciences (Suzhou, China). The cells were seeded in a specific culture medium (HUXMA-90011, HUXMD-90011, and HUXUC-90011, respectively; Cyagen Biosciences) and maintained at 37 °C and 5% CO2. The culture medium was changed every day. MSCs of the third passage were used in the following experiments.
To evaluate their multiple differentiation potential, MSCs were induced to differentiate by culturing in osteogenic, adipogenic, and chondrogenic differentiation medium (Cyagen), which confirmed by Alizarin red, Oil red O, and Alcian blue staining. MSC phenotypic analysis was performed using flow cytometry (BD Biosciences, San Jose, CA, USA), and the percentage of cells expressing mesenchymal markers—CD105, CD29, CD73, CD34, CD45, CD11b, and CD44 (Abcam, Cambridge, UK), was determined.
To evaluate their multiple differentiation potential, MSCs were induced to differentiate by culturing in osteogenic, adipogenic, and chondrogenic differentiation medium (Cyagen), which confirmed by Alizarin red, Oil red O, and Alcian blue staining. MSC phenotypic analysis was performed using flow cytometry (BD Biosciences, San Jose, CA, USA), and the percentage of cells expressing mesenchymal markers—CD105, CD29, CD73, CD34, CD45, CD11b, and CD44 (Abcam, Cambridge, UK), was determined.
3
Multilineage Differentiation of MSCs
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MSCs derived from different tissues (BM, AT, and UC) were obtained from Cyagen Biosciences (Suzhou, China). The cells were seeded in speci c culture medium (HUXMA-90011, HUXMD-90011, and HUXUC-90011, respectively; Cyagen Biosciences) and maintained at 37 °C and 5% CO 2 . The culture medium was changed every day. MSCs of the third passage were used in the following experiments.
To evaluate their multiple differentiation potential, MSCs were induced to differentiate by culturing in osteogenic, adipogenic, and chondrogenic differentiation medium (Cyagen), which con rmed by Alizarin red, Oil red O, and Alci an blue staining. MSCs phenotypic analysis was performed using ow cytometry (BD Biosciences, San Jose, CA, USA) and the percentage of cells expressing mesenchymal markers -CD105, CD29, CD73, CD34, CD45, CD11b, and CD44 (Abcam, Cambridge, UK) was determined.
To evaluate their multiple differentiation potential, MSCs were induced to differentiate by culturing in osteogenic, adipogenic, and chondrogenic differentiation medium (Cyagen), which con rmed by Alizarin red, Oil red O, and Alci an blue staining. MSCs phenotypic analysis was performed using ow cytometry (BD Biosciences, San Jose, CA, USA) and the percentage of cells expressing mesenchymal markers -CD105, CD29, CD73, CD34, CD45, CD11b, and CD44 (Abcam, Cambridge, UK) was determined.
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