Mice were deeply anesthetized with isoflurane and transcardially perfused with 1x phosphate buffered saline (PBS) followed by 4% paraformaldehyde (PFA) in 1× PBS. Whole brains were removed and post-fixed in 4% PFA until further processing. Samples were cryoprotected in 30% sucrose in 11× PBS, frozen on dry ice, and mounted in Cryo-Gel Tissue Embedding Medium (Leica Biosystems GmBH, Wetzlar, Germany). Coronal brain sections (50 μm thick) were collected in 1× PBS using a Leica Biosystems CM3050 S cryostat. Sections were mounted with DAPI-Fluoromount-G aqueous mounting medium (Electron Microscopy Sciences, PA, USA) onto Superfrost Plus glass slides (Fisher Scientific, NH, USA). Images were taken with an AxioZoom.V16 fluorescence microscope (Carl Zeiss Microscopy LLC, NY, USA).
Cryo gel tissue embedding medium
Manufactured by Leica
Sourced in Germany
Cryo-Gel Tissue Embedding Medium is a product designed for the preparation of frozen tissue sections. It is used to embed and support tissue samples during the cryosectioning process. The medium provides a stable, uniform matrix to hold the tissue in place, enabling thin, high-quality sections to be obtained for microscopic analysis.
Automatically generated - may contain errors
2 protocols using cryo gel tissue embedding medium
1
Perfusion, Cryosectioning, and Imaging of Murine Brains
2
Perfusion, Cryosectioning, and Imaging of Murine Brains
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Mice were deeply anesthetized with isoflurane and transcardially perfused with 1x phosphate buffered saline (PBS) followed by 4% paraformaldehyde (PFA) in 1× PBS. Whole brains were removed and post-fixed in 4% PFA until further processing. Samples were cryoprotected in 30% sucrose in 11× PBS, frozen on dry ice, and mounted in Cryo-Gel Tissue Embedding Medium (Leica Biosystems GmBH, Wetzlar, Germany). Coronal brain sections (50 μm thick) were collected in 1× PBS using a Leica Biosystems CM3050 S cryostat. Sections were mounted with DAPI-Fluoromount-G aqueous mounting medium (Electron Microscopy Sciences, PA, USA) onto Superfrost Plus glass slides (Fisher Scientific, NH, USA). Images were taken with an AxioZoom.V16 fluorescence microscope (Carl Zeiss Microscopy LLC, NY, USA).
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