Pancol

Manufactured by PAN Biotech

Pancol is a laboratory equipment product designed for the concentration and evaporation of liquid samples. It operates using a rotary evaporator mechanism to efficiently remove solvents from samples, enabling researchers to isolate and concentrate target compounds or substances.

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Lab products found in correlation

Pharm lyse buffer, by BD (1 mentions) Cd25 pe cy7, by Thermo Fisher Scientific (1 mentions) Cd4 pacific blue, by Thermo Fisher Scientific (1 mentions) Gallios cytometer, by Beckman Coulter (1 mentions) Cd161 percpcy5, by BioLegend (1 mentions) Kaluza flow cytometry software, by Beckman Coulter (1 mentions) Cd20 pacific blue, by BioLegend (1 mentions) Pharm lyse, by BD (1 mentions)

4 protocols using pancol

PBMC Isolation from Buffy Coats

Cited 1 time

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PBMCs were isolated from fresh buffy coats obtained from healthy donors by means of density gradient centrifugation (Pancol, PAN Biotech), as previously described [28 (link)]. Pharm lyse buffer (BD, Franklin Lakes, NJ, USA) was used to remove residual red blood cells when needed. PBMC numbers were evaluated using a Bürker chamber. Freshly isolated PBMCs were resuspended in complete culture medium and used immediately for further research. Buffy coats were collected upon the approval of the Ethics Committee of the Medical University of Bialystok.

Holl J., Pawlukianiec C., Corton Ruiz J., Groth D., Grubczak K., Hady H.R., Dadan J., Reszec J., Czaban S., Kowalewski C., Moniuszko M, & Eljaszewicz A. (2021). Skin Substitute Preparation Method Induces Immunomodulatory Changes in Co-Incubated Cells through Collagen Modification. Pharmaceutics, 13(12), 2164.

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Multiparameter flow cytometry analysis of lymphocyte subsets after allo-SCT

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T-cell (CD3/CD4), NK-cell, and B-cell absolute numbers were analyzed by our routine central laboratory using flow cytometry and the TruCount system (Becton & Dickinson, www.bd.com). For analysis of T-regulatory cells and CD3+ CD161+ NK/T cells, whole blood (30 mL) was collected in EDTA tubes at day+30, +90, and +150 after allo-SCT. Peripheral blood mononuclear cells were isolated by density-gradient centrifugation (Pancol^®, www.pan-biotech.com). Cells were stained with the following fluorescence-labeled antibodies: CD20 Pacific Blue and CD161 PerCP-Cy5.5 (www.biolegend.com), CD4 Pacific Blue, CD25 PE-Cy7, CD127 AlexaF700, CD3 APC-H7 (www.bd.com), CD19 Alexa780 APC (www.eBioscience.com), CD45 Pacific Orange (www.lifetechnologies.com).
Flow cytometry was performed using a Gallios^® cytometer (Beckman Coulter, www.beckmancoulter.com). Data were analyzed by Kaluza^® flow cytometry software (Beckman Coulter).

Holtick U., Chemnitz J.M., Shimabukuro-Vornhagen A., Theurich S., Chakupurakal G., Krause A., Fiedler A., Luznik L., Hellmich M., Wolf D., Hallek M., von Bergwelt-Baildon M, & Scheid C. (2015). OCTET-CY: a phase II study to investigate the efficacy of post-transplant cyclophosphamide as sole graft-versus-host prophylaxis after allogeneic peripheral blood stem cell transplantation. European journal of haematology, 96(1), 27-35.

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Isolating and Stimulating PBMCs

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Blood samples from healthy donors were provided by the Lyon Blood Bank. Peripheral Blood Mononuclear Cells (PBMC) were obtained by performing a density gradient centrifugation (Pancol, Pan-Biotech). Remaining red blood cells were removed using a lysis solution (BD Pharm Lyse, BD Biosciences) and cells were maintained in culture at 37°C with 5% CO2 in RPMI medium supplemented with 10% heat inactivated fetal calf serum (FCS), 200 UI/mL of penicillin and 200 μg/mL of streptomycin. After one-hour, non-adherent cells were harvested by thorough washing with PBS. Adherent cells were stimulated with unformulated LPS or Lipo LPS for 1 ½ hours, at various concentrations.

Chettab K., Fitzsimmons C., Novikov A., Denis M., Phelip C., Mathé D., Choffour P.A., Beaumel S., Fourmaux E., Norca P., Kryza D., Evesque A., Jordheim L.P., Perrial E., Matera E.L., Caroff M., Kerzerho J, & Dumontet C. (2023). A systemically administered detoxified TLR4 agonist displays potent antitumor activity and an acceptable tolerance profile in preclinical models. Frontiers in Immunology, 14, 1066402.

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PBMC Isolation from Buffy Coats

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Blood samples were obtained from buffy coats of healthy donors under an agreement with the Etablissement Français du Sang (EFS)—Midi-Pyrénées, in accordance with the EFS ethical guidelines. PBMC were isolated after centrifugation in a density gradient (Pancol, PAN Biotech) and frozen in DMSO before use. Experiments were performed after thawing except for phospho-flow and microarray analyses where fresh cells were used.

Pavlovic M., Gross C., Chili C., Secher T, & Treiner E. (2020). MAIT Cells Display a Specific Response to Type 1 IFN Underlying the Adjuvant Effect of TLR7/8 Ligands. Frontiers in Immunology, 11, 2097.

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