Folic acid, dimethyl sulfoxide (DMSO) and insulin were purchased from Sigma–Aldrich (St. Louis, MO, USA). LY294002 was purchased from Merck Biosciences (New Jersey, USA). KC7F2 was purchased from Millipore (Billerica, MA, USA). Dimethyloxallyl glycine (DMOG) was purchased from Cayman Chemical (Ann Arbor, MI, USA). RPMI 1640, fetal bovine serum, antibiotics, and all other cell culture reagents were purchased from GIBCO/BRL Life Technologies (Grand Island, NY). The HIF-1α antibody was purchased from BD Biosciences (New Jersey, USA). The phospho-Akt (Ser 473) and Akt antibodies were purchased from Cell Signaling Technology (Danvers, USA). β-actin and Lamin B1 antibodies were obtained from Proteintech Group Inc (Chicago, IL, USA).
Lamin b1 antibody
Manufactured by Proteintech
Sourced in United States, China
The Lamin B1 antibody is a laboratory reagent used for the detection and analysis of the Lamin B1 protein in biological samples. Lamin B1 is a structural protein that is a component of the nuclear lamina, which provides structural support and organization to the cell nucleus. This antibody can be used in various immunoassay techniques, such as Western blotting and immunohistochemistry, to investigate the expression and localization of Lamin B1 in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Proteintech (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Cell culture reagents, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Phospho akt ser473, by Cell Signaling Technology (1 mentions)
Insulin, by Merck Group (1 mentions)
Folic acid, by Merck Group (1 mentions)
Ly294002, by Merck Group (1 mentions)
Hif 1α antibody, by BD (1 mentions)
Rapamycin, by Thermo Fisher Scientific (1 mentions)
Alginate, by Merck Group (1 mentions)
Fitc phalloidin, by Merck Group (1 mentions)
Foetal bovine serum (fbs), by Sartorius (1 mentions)
Fitc lps, by Merck Group (1 mentions)
Phosphor erk p erk, by Cell Signaling Technology (1 mentions)
β tubulin, by Proteintech (1 mentions)
Tak 242, by Thermo Fisher Scientific (1 mentions)
Sb 20358, by Selleck Chemicals (1 mentions)
F4 80, by Abcam (1 mentions)
Ly294002, by Selleck Chemicals (1 mentions)
9 protocols using lamin b1 antibody
1
Hypoxia Signaling Pathway Regulation
2
Molecular Pathways Modulation in Inflammation
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Alginate, LPS, PMB, FITC-phalloidin, FITC-LPS and 4′,6-diamidino-2-phenylindole (DAPI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). RPMI-1640 medium, Dulbecco’s modified Eagle’s medium (DMEM), penicillin and streptomycin were purchased from Hyclone (Logan, UT, USA). Foetal bovine serum (FBS) was obtained from Biological Industries (Beit-Haemek, Israel). Anti-mouse TLR4, MD2, MyD88, F4/80 and Ly6G antibodies were from purchased Abcam (Cambridge, UK). Antibodies against Akt, phosphor-Akt (p-Akt), NF-κB p65, IκB, phosphor-IκB (p-IκB), mTOR, phosphor-mTOR (p-mTOR), p70 S6 kinase (p70 S6K), phosphor-p70 S6K (p-p70 S6K), p38, phosphor-p38 (p-p38), JNK, phosphor-JNK (p-JNK), ERK, phosphor-ERK (p-ERK) and iNOS were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-β-actin, -β-tubulin, -GAPDH and -Lamin B1 antibodies were purchased from Proteintech (Hubei, China). Inhibitors LY 294002, SB 20358, SP 600125 and PD 98059 were obtained from Selleck (Shanghai, China) and TAK-242 and rapamycin from Invitrogen (Carlsbad, CA, USA). The other chemicals were all purchased from Macklin Biochemical Technology (Shanghai, China).
3
HMGB1-Mediated JAK2/STAT1 Signaling
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LPS 055: B5 was obtained from Sigma-Aldrich (St. Louis, MO, USA). AZD180 was purchased from SelleckChem (Houston, TX, USA). Mouse HMGB1 antibody was purchased from Abcam (Cambridge, MA, USA). β-Actin and Lamin B1 antibodies were obtained from Proteintech (Wuhan, Hubei, China). Antibodies against phosphorylated JAK2 (p-JAK2; Tyr1007/1008), JAK2, phosphorylated STAT1 (p-STAT1; Tyr701), cleaved caspase 3 (Asp175; 5A1E), caspase 3, and LC3B were obtained from Cell Signaling Technology (Boston, MA, USA). Horseradish peroxidase-conjugated goat anti-rabbit antibody and enhancer chemiluminescent reagent were purchased from Piece Biotechnology (Rockford, IL, USA). Polyvinylidene difluoride membranes were obtained from Millipore (Bedford, MA, USA).
4
Immunoblotting and Immunofluorescence Antibodies for Cell Signaling
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Antibodies against Notch1 ICD (D1E11, 1:100 IF, 1:1000 WB), Notch1 V1744 (D3B8, 1:500 WB), EGFR (D38B1, 1:100 IF, 1:1000 WB), pEGFR (D7A5, 1:1000 WB), GFP (D5.1, 1:1000 WB), non-phospho (Active) β-Catenin (D13A1, 1:1000 WB), GAPDH (14C10, 1:10000 WB) and YAP (D8H1X, 1:200 IF) were from Cell Signaling Technologies. β-catenin antibody (14, 1:1000 WB) was from BD Biosciences. E-cadherin antibody (HECD-1, 1:1000 IF, 1:1000 WB) was from Takara Bio. Notch1 ECD (ABS90, 1:1000) was from Millipore. FAM83H antibody (1:1000 WB) was from Bethyl Laboratories. Lamin B1 antibody (12987–1-AP, 1:1000) was from Proteintech. Rhodamine phalloidin and Alexa Fluor 488, 568 and 647 goat anti-mouse and anti-rabbit IgG secondary antibodies (1:400) were from Invitrogen. Alexa Fluor 647 azide and EdU cell proliferation kit was from Invitrogen. Hoescht and DAPT were from Sigma.
5
Ferroptosis Pathway Regulation Assay
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Sigma‐Aldrich (MO, USA) supplied Fer‐1 and erastin, while Proteintech Group, Inc. provided the primary antibodies: anti‐YAP (20536‐1‐AP,1:1000), anti‐RUNX2 (AF5186,1:1000; Affinity Biosciences), anti‐TEAD2 (21159‐1‐AP,1:1000; Proteintech Group, Inc.), Lamin B1 antibody (12987‐1‐AP,1:1000; Proteintech Group, Inc.), anti‐β‐catenin (20536‐1‐AP,1:1000; Proteintech Group, Inc.), anti‐GPX4 (ab125066,1:1000; Abcam) and anti‐ACSL4 (ab177958,1:1000; Abcam). Cell Signaling Technology (MA, USA) supplied all the secondary antibodies.
6
Nuclear and Cytoplasmic Fractionation of HCC
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Nuclear and cytoplasmic fractionations of tumor tissues of HCC were extracted using the NE-PER Nuclear and Cytoplasmic Extraction Reagents kit (Thermo Fisher Scientific, Rockford, USA) according to the manufacturer's protocol. Protein concentration was quantified using Pierce BCA Protein Assay. Ten microgram of each cytoplasmic and nuclear extract sample was analyzed for western blot with TERT antibody (diluted 1:500, Thermo Fisher Scientific, Rockford, USA), laminB1 antibody (diluted 1:1000, Proteintech, Hubei, China) and β-actin antibody (diluted 1:1000, HuaAn Biotechnology, Zhe Jiang, China). β-actin was used as cytoplasmic loading control for cytoplasmic extracts and laminB was used as nuclear loading control for nuclear extracts.
7
Multicolor Immunofluorescence Imaging Protocol
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Primary Antibodies: anti-CENPA (MBL international 1:500, Code # D115-3), anti-CENPB primary antibody (Santa Cruz Biotechnology, CENPB Antibody (C-10) 1:50: sc-376392, pSer139-γ-H2AX antibody (Cell Signaling 1:400, Cat # 9718), BANF1/BAF antibody (abcam,1:100, EPR7668), MHCII DRB1 antibody (Abclonal, 1:100, Cat # A7685), Lamin B1 antibody (Proteintech, 1:1000, Cat # 66095-1-lg), cGAS antibody (Abclonal, 1:100, Cat# A8335), p(Ser396)-IRF antibody (Cell Signaling, 1:100, Cat # 29047), p(Ser536)-p65 antibody (Abclonal, 1:100, Cat # AP0123), MHCII DRB5 antibody (Abclonal, 1:100, Cat # A12726), anti-GAPDH antibody (Abcam, ab 9484), anti-Histone H3 (tri methyl K9) antibody (Abcam, ab8898), anti-CENPA antibody [3-19] (Abcam, ab13939).
Secondary Antibodies: Alexa Flour 594 rabbit anti-mouse IgG 1:1000, Thermo Fischer Scientific, Cat No A27027, Alexa Flour 488 rabbit anti-mouse IgG 1:1000, Thermo Fischer Scientific, Cat No A27023. Alexa fluor 647 Goat anti-rabbit IgG, ThermoFisher, 1:1000, Cat # A32733TR
Secondary Antibodies: Alexa Flour 594 rabbit anti-mouse IgG 1:1000, Thermo Fischer Scientific, Cat No A27027, Alexa Flour 488 rabbit anti-mouse IgG 1:1000, Thermo Fischer Scientific, Cat No A27023. Alexa fluor 647 Goat anti-rabbit IgG, ThermoFisher, 1:1000, Cat # A32733TR
8
Lamin B1 Immunoprecipitation in CRC Cells
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CRC cells were transfected in six-well plates for 72h. Then the cells were lysed with mammalian cell lysis reagent (Pioneer Biotechnology, K0301), followed by overnight incubation at 4℃ with lamin B1 antibody (Proteintech, 12987-1-AP). The cell extracts were then incubated with protein A and G beads for 3h. The beads were washed three times with Tris-HCL buffered saline, and the immunoprecipitation complexes were subjected to SDS-PAGE.
9
Protein Expression Analysis in Exosomes
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Cells were lysed in RIPA buffer (P0013C, Beyotime, Shanghai, China) with a protease inhibitor cocktail (P8340, Sigma‐Aldrich). The total proteins of lysates or exosomes were separated by SDS polyacrylamide gels and transferred onto PVDF membranes (Millipore, USA). Then, these membranes were blocked with 5% BSA for one hour at room temperature and incubated with primary antibodies overnight at 4°C. Afterwards, the secondary antibodies were added. The blots were observed using the Azure Biosystems C300. The antibodies are listed, as follows: rabbit anti‐Tyrosinase (ab170905; Abcam, Cambridge, MA, USA), rabbit anti‐TRP1 (ab178676, Abcam), rabbit anti‐TRP2/DCT (ab221144, Abcam), rabbit anti‐MITF (ab140606, Abcam), rabbit anti‐β‐catenin (51067‐2‐AP; Proteintech, Wuhan, China), rabbit anti‐SOX1 (20744‐1‐AP, Proteintech), rabbit anti‐CD63 (25682‐1‐AP, Proteintech), rabbit anti‐TSG101 (14497‐1‐AP, Proteintech), Lamin B1 antibody (12987‐1‐AP, Proteintech) and β‐actin (no.3700, Cell Signaling Technology, Danvers, MA, USA).
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