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Anti ku80

Manufactured by Santa Cruz Biotechnology
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Anti-Ku80 is a laboratory reagent used in protein analysis and research. It functions as an antibody that specifically binds to the Ku80 protein, which is involved in DNA repair and telomere maintenance. This product can be used in techniques such as immunoprecipitation, Western blotting, and immunohistochemistry to detect and study the Ku80 protein.

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Lab products found in correlation

Anti tubulin, by Merck Group (4 mentions) Anti ku70, by Santa Cruz Biotechnology (3 mentions) Anti flag m2, by Merck Group (2 mentions) Hrp linked anti mouse igg, by Cell Signaling Technology (2 mentions) Anti actin, by Merck Group (1 mentions) Hrp conjugated anti mouse anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Anti lamin b1, by Santa Cruz Biotechnology (1 mentions) Anti xlf, by Fortis Life Sciences (1 mentions) Anti dna pkcs, by Santa Cruz Biotechnology (1 mentions) Anti phospho chk2 t68, by Cell Signaling Technology (1 mentions) Anti phospho h2ax s139, by Merck Group (1 mentions) Anti phospho atm s1981, by Abcam (1 mentions) Anti xrcc4, by Santa Cruz Biotechnology (1 mentions) Anti kap1, by Fortis Life Sciences (1 mentions) Anti kap1 phospho s824, by Fortis Life Sciences (1 mentions) Anti dna pkcs phospho s2056, by Abcam (1 mentions) Anti histone h3 antibody, by BioLegend (1 mentions) Anti gfp, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti flag, by Merck Group (1 mentions)

Spelling variants of this product

Mouse anti-Ku80 monoclonal antibody (B-4, (2 citations) Anti-KU80 (C-20, (2 citations)

4 protocols using anti ku80

1

Antibodies for DNA Repair Protein Analysis

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Rabbit polyclonal antibodies: anti-Ligase IV against amino acids 1–240, anti-XRCC4 (Serotec), anti-Artemis raised against full-length recombinant Artemis, anti-XLF (Bethyl Laboratories, Inc), anti-DNA-PKcs (Santa Cruz Biotechnology, Inc), anti-Ku70, anti-Ku80 (Santa Cruz Biotechnology, Inc) and anti-Phospho-p53 (Ser315) (Cell Signaling). Goat polyclonal antibodies: anti-Lamin B1 (Santa Cruz Biotechnology, Inc). Mouse monoclonal antibodies: anti-Flag M2 (Sigma-Aldrich), anti-p53 (BioLegend), anti- -Tubulin (Sigma) and anti- -Actin (Sigma-Aldrich). Secondary antibodies used are as follows: HRP conjugated anti-mouse, anti-rabbit IgG (Thermo Fisher Scientific) and HRP conjugated anti-goat (Santa Cruz Biotechnology, Inc).
Francis D.B., Kozlov M., Chavez J., Chu J., Malu S., Hanna M, & Cortes P. (2014). DNA Ligase IV regulates XRCC4 nuclear localization. DNA repair, 21, 36-42.
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2

Immunoblotting of DNA Repair Proteins

Cited 1 time
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Immunoblotting was performed as previously described (24 (link)). The following antibodies were used in this study: anti-DNA-PKcs phospho-T4102 (made in this study as described above), anti-DNA-PKcs phospho-S2056 (Abcam, ab124918), anti-ATM phospho-S1981 (Abcam, ab81292), anti-CHK2 phospho-T68 (Cell Signaling, 2197), anti-LIG4 (Cell Signaling, 14649), anti-Ku80 (Santa Cruz, sc-17789), anti-Ku70 (Santa Cruz, sc-515736), anti-XRCC4 (Santa Cruz, sc-271087), anti-XLF (Santa Cruz, sc-166488), anti-GFP (Santa Cruz, sc-8334), anti-KAP1 (Bethyl Laboratories A300-274A), anti-KAP1 phospho-S824 (Bethyl Laboratories, A300-767A), anti-tubulin (Sigma-Aldrich, T5168), anti-FLAG M2 (Sigma-Aldrich, F1804), anti-phospho-H2AX (S139) (EMD Millipore, 05-636), and anti-Histone H3 antibody (Biolegend, 819411). Mouse monoclonal antibodies against DNA-PKcs (Clone # 25-4) were produced in house. Secondary antibodies used include anti-mouse IgG (HRP-linked) (Cell Signaling, 7076) and anti-rabbit IgG (HRP-linked) (Cell Signaling, 7074). Image J (Version 1.53e) was used to quantify the intensities of protein bands in the immunoblots.
Lu H., Zhang Q., Laverty D.J., Puncheon A.C., Augustine M.M., Williams G.J., Nagel Z.D., Chen B.P, & Davis A.J. (2023). ATM phosphorylates the FATC domain of DNA-PKcs at threonine 4102 to promote non-homologous end joining. Nucleic Acids Research, 51(13), 6770-6783.
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3

Immunoblotting protocol for protein detection

Cited 1 time
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Immunoblotting was performed as previously described (7 (link)). The following commercial antibodies were used: anti-tubulin (Sigma, T5168), anti-Ku70 (Santa Cruz, SC-17789) and anti-Ku80 (Santa Cruz, SC-515736). The secondary antibody used was the anti-mouse IgG (HRP-linked) from Cell Signaling Technology.
Saha J., Bae J., Wang S.Y., Lu H., Chappell L.J., Gopal P, & Davis A.J. (2021). Ablating putative Ku70 phosphorylation sites results in defective DNA damage repair and spontaneous induction of hepatocellular carcinoma. Nucleic Acids Research, 49(17), 9836-9850.
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4

Comprehensive Protein Analysis by Western Blot

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Western blotting was performed according to standard procedures. Antibodies used were anti-SIRT7 (Santa Cruz Biotechnology, sc-365344, 1:500), anti-HA (MBL, M180-3, 1:2,000), anti-Flag (Sigma, F3165, 1:10,000), anti-β-actin (Sigma, A1978, 1:10,000), anti-tubulin (Sigma, clone B-5-1-2, T6074, 1:50,000), anti-SIRT6 (Abgent, AP-6245a, 1:500), anti-PARP1/2 (Santa Cruz Biotechnology, sc-7150, 1:5,000), anti-Ku80 (Santa Cruz Biotechnology, sc-5280, 1:2,000), anti-BRCA1 (Proteintech, 22362-1-AP, 1:1,000), anti-γH2AX (Millipore, 05-636, 1:2,000), anti-H2AX (Abcam, ab11175, 1:2,000) anti-pan-acetylation (PTM BioLabs, PTM-105, 1:1,000), anti-pan-succinylation (PTM BioLabs, PTM-401, 1:1,000), anti-H3K122succ (1:4,000), anti-H2BK120succ (1:8,000), anti-H3K122ac (Abcam, ab33309, 1:2,000), anti-H3K18ac (PTM BioLabs, PTM-114, 1:1,000), anti-H3 (Abcam, ab1791, 1:100,000) and anti-rabbit (Jackson ImmunoResearch, 115-035-003, 1:8,000) or anti-mouse (Jackson ImmunoResearch, 111-035-003, 1:8,000) secondary antibodies conjugated to horseradish peroxidase. The bands were quantified by densitometry with ImageJ software. Uncropped scans of the most important blots are shown in Supplementary Fig. 11.
Li L., Shi L., Yang S., Yan R., Zhang D., Yang J., He L., Li W., Yi X., Sun L., Liang J., Cheng Z., Shi L., Shang Y, & Yu W. (2016). SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability. Nature Communications, 7, 12235.
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