Immunohistochemical and immunofluorescent staining of allografts and colon biopsies were performed as previously described.21 (link) Cytokeratins, Ly6A/E and Dclk1 were stained using mouse anti-pancytokeratin monoclonal antibody (Novus Biologicals), rat anti-Ly6A/E (BD BioSciences) and rabbit polyclonal antibody to Dclk1 (Abcam). Rabbit anti-nitric oxide synthase 2 (Enzo Life Sciences), anti-arginase 1 (Sigma), and anti-MSH2 (Santa Cruz Biotechnology) polyclonal antibodies were used for Western blots.
Anti arginase 1
Manufactured by Merck Group
Sourced in United States, United Kingdom
Anti-arginase 1 is a laboratory equipment product that functions as an inhibitor of the enzyme arginase 1. Arginase 1 is an enzyme involved in the urea cycle. The primary role of anti-arginase 1 is to inhibit the activity of arginase 1 in experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Anti msh2, by Santa Cruz Biotechnology (1 mentions)
Anti nf κb p65, by Merck Group (1 mentions)
Acquity uplc beh c18 column, by Waters Corporation (1 mentions)
Collagenase 2, by Merck Group (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Lightcycler 480 sybr green 1 master kit, by Roche (1 mentions)
Fitc conjugated isolectin b4, by Merck Group (1 mentions)
3 protocols using anti arginase 1
1
Immunostaining of Allografts and Biopsies
2
Quantitative Analysis of Inflammatory Mediators
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LC grade solvents (Biosolve, Dieuze, France), Cleanert S, C18 solid-phase extraction columns (500 mg/ 3ml), Waters ACQUITY UPLC BEH- C18 column (2.1 mm X 50mm, 1.7μm); deuterated (d) internal standards 17(S)-Resolvin D1-d5,EPA, DHA was purchased from Cayman Chemical (Ann Arbor, MI). All the ELISA kits for cytokine analysis were purchased from e-Bioscience, USA. Mouse insulin ELISA kit was obtained from Mercer EXPert, USA. Collagenase II, anti-arginase 1, anti-β-actin and anti-NFκB/P65 antibodies were purchased from Sigma-Aldrich Co. (USA). Light Cycler® 480 SYBR Green I Master kit was purchased from Roche, USA.RNA isolation kit was from MP Biomedicals, USA. Membrane filters (100 μm) were purchased from Millipore (USA). All other chemicals were of analytical grade and procured from standard commercial sources in India.
3
Rat Microglia and Neuron Cultures
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Animals and materialsSprague Dawley rat pups (5-6 day old) were bred and reared in house from stock animals (Charles River UK, Kent, UK), and were sacrificed in accordance with schedule 1 of the Animals Scientific Procedures Act (1986) UK. Primary cultures of rat microglia and primary cultures of mixed glia-cerebellar granule neurons were prepared and seeded as previously described [18] .The cells were used after 1 day in vitro (DIV; microglia) or at least 7 DIV (glialneuronal cultures). Cell culture materials were from Invitrogen (Paisley, UK), FITC-conjugated isolectin B4 (ILB4) a marker of microglia [19] , was from Sigma and anti-arginase-1, a marker for the M2 microglial phenotype characterized as a protective, regenerative phenotype (16;17) was from Santa Cruz Biotech (USA).
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