Blood culture flasks were analyzed after bacT/ALERT®3D system flagged a blood as positive. The blood culture flasks were analyzed by Gram staining followed by subculture on an appropriate solid agar medium (aerobic Columbia agar 5% sheep blood, chocolate agar, MacConkey agar, bioMérieux, France) following 18–24 h incubation at 35°C and 5% CO2 atmosphere. The colonies grown on overnight agar plate incubation were spotted onto a target slide and prepared according to the manufacturer's instructions for analysis using VITEK MS® system software version 3.0 (bioMérieux). This system was termed the standard identification method (sID). The colonies grown on overnight agar plates were also used for the preparation of the inoculum disk diffusion test (Kirby–Bauer disk diffusion susceptibility test protocol), according to CLSI [6 ]. This test was termed the standard disk diffusion method (sAST). The bacterial identification and antimicrobial resistance detection results obtained using this conventional workflow were used for comparison in the data analyses.
Vitek ms system software version 3
Manufactured by bioMérieux
The VITEK MS® system software version 3.0 is a laboratory equipment used for microbial identification. The core function of this software is to analyze mass spectrometry data to accurately identify a wide range of microorganisms.
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Lab products found in correlation
2 protocols using vitek ms system software version 3
1
Bacterial Identification and Antibiotic Resistance Testing
2
Automated Bacterial Identification and Antibiotic Susceptibility
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Blood culture bottles were analyzed after the bacT/ALERT® 3D system flagged them as positive. Samples were analyzed by Gram staining followed by subculture on an appropriate solid agar medium (Chocolate agar or MacConkey agar, bioMérieux) after 18-24 hours of incubation at 35°C. Colonies grown on the plate overnight during incubation were mounted on a slide and prepared for analysis using VITEK MS® System software version 3.0 (bioMérieux) according to the manufacturer's instructions. The identification was considered correct if the confidence values were between 60 and 99.9, as indicated by the manufacturer. This system was referred to as sID. Colonies grown overnight on agar plates were also used to prepare an inoculum for the disk diffusion test (Kirby-Bauer disk diffusion test protocol) according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST), version 10. This disk diffusion method was referred to as sAST. Bacterial identification and antimicrobial resistance results obtained using this conventional workflow were used for comparison in data analysis. Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were used as quality control.
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