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Ex tag dna polymerase

Manufactured by Takara Bio
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Sourced in Germany

ExTaq DNA polymerase is a thermostable DNA polymerase enzyme used for DNA amplification in polymerase chain reaction (PCR) experiments. It exhibits high thermal stability and DNA synthesis activity.

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Lab products found in correlation

Abi prism 3730, by Thermo Fisher Scientific (5 mentions) Mutation surveyor, by SoftGenetics (5 mentions) Hiseq 2000 sequencer, by Illumina (3 mentions) Tgradient thermocycler, by Analytik Jena (1 mentions) Qiaquick pcr purification kit, by Qiagen (1 mentions)

6 protocols using ex tag dna polymerase

1

Molecular Biology Techniques Protocol

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Genomic DNA extractions, plasmid preparations, PCR reactions, ligations, transformations, and other standard molecular biology techniques were carried out as described elsewhere (Sambrook & Russell 2012 ) or following the instructions of the supplier. PCR experiments were carried out by using T Gradient thermocycler (Biometra, Germany) and Ex Tag DNA polymerase (Takara Bio, Inc., Japan). Genomic DNA extracted from S. elodea was used as the template. PCR products were purified using QIAquick PCR purification kit (Qiagen, Germany). E. coli and S. elodea cells were transformed by electroporation using Electro Cell Manipulator (BTX Technologies Inc., USA).
Lee S.Y., Ahn J.Y., Kim M., Sekhon S.S., Cho S.J., Kim Y.C, & Kim Y.H. (2017). Phenotypic and proteomic analysis of positively regulated gellan biosynthesis pathway in Sphingomonas elodea. Animal Cells and Systems, 21(2), 115-123.
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2

Targeted Exome Sequencing for Mutation Identification

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Genomic DNA of the proband was subjected to TES. Filtered candidate variants identi ed by an Illumina HiSeq 2000 sequencer were con rmed by Sanger sequencing. The coding exons containing the detected mutations were ampli ed using Ex Tag DNA polymerase (Takara, Dalian). The puri ed PCR samples were sequenced using an ABI PRISM 3730 genetic analyser (Applied Biosystems; Thermo Fisher Scienti c, Inc.), and then sequence traces were analysed with Mutation Surveyor (Softgenetics, PA). The mutation was con rmed in the family members by the same procedure. Multiple sequence alignments were performed using ClustalW2 with the default setting (http://www.ebi.ac.uk/Tools/clustalw2/). Protein structures were determined by SMART (http://smart.emblheidelberg.de). The variation in the 3D structure of the protein caused by gene mutation was analysed using Protein Data Bank (PDB) and the homology modelling software Swiss-Model. All genomic DNA samples were collected after obtaining informed consent.
Xing D., Zhou H., Yu R., Wang L., Hu L., Li Z., & Li X. (2020). Targeted exome sequencing identified a novel USH2A mutation in a Chinese Usher syndrome family: a case report.
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3

Targeted Exome Sequencing for Variant Identification

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Genomic DNA of the proband was subjected to TES. Filtered candidate variants identi ed by anIllumina HiSeq 2000sequencer were con rmed by Sanger sequencing. The coding exons containing the detected mutations were ampli ed using Ex Tag DNA polymerase (Takara, Dalian). The puri ed PCR samples were sequenced using the ABI PRISM 3730 genetic analyser (Applied Biosystems; Thermo Fisher Scienti c, Inc.), and then sequence traces were analysed with the Mutation Surveyor (Softgenetics, PA). The mutation in the family members was con rmed by the same procedure.
Multiple sequence alignments were performed using ClustalW2 with the default setting (http://www.ebi.ac.uk/Tools/clustalw2/).Protein structures were determined by SMART (http://smart.emblheidelberg.de). The 3D structure of the protein variation caused by gene mutation was analysed using Protein Data Bank(PDB) and the homology modelling software Swiss-Model. We collected all genomic DNA samples upon informed consent.
Xing D., Zhou H., Yu R., Wang L., Hu L., Li Z., & Li X. (2020). Targeted exome sequencing identified a novel USH2A mutation in a Chinese Usher syndrome family: a case report.
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4

Targeted Exome Sequencing and Variant Analysis

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Genomic DNA of the proband was subjected to TES. Filtered candidate variants identi ed by anIllumina HiSeq 2000sequencer were con rmed by Sanger sequencing. The coding exons containing the detected mutations were ampli ed using Ex Tag DNA polymerase (Takara, Dalian). The puri ed PCR samples were sequenced using the ABI PRISM 3730 genetic analyser (Applied Biosystems; Thermo Fisher Scienti c, Inc.), and then sequence traces were analysed with the Mutation Surveyor (Softgenetics, PA). The mutation in the family members was con rmed by the same procedure. Multiple sequence alignments were performed using ClustalW2 with the default setting (http://www.ebi.ac.uk/Tools/clustalw2/).Protein structures were determined by SMART(http://smart.emblheidelberg.de).
The 3D structure of the protein variation caused by gene mutation was analysed using Protein Data Bank(PDB) and the homology modelling software Swiss-Model(Biasini et al., 2014).We collected all genomic DNA samples upon informed consent.
3 Results
Xing D., Zhou H., Yu R., Wang L., Hu L., Li Z., & Li X. (2020). Targeted Exome Sequencing Identified a Novel USH2A Mutation in a Chinese Usher Syndrome Family.
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5

Genetic Variant Identification and Analysis

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Genomic DNA of the proband was subjected to TES. Filtered candidate variants identified by an Illumina HiSeq 2000 sequencer were confirmed by Sanger sequencing. The coding exons containing the detected mutations were amplified using Ex Tag DNA polymerase (Takara, Dalian). The purified PCR samples were sequenced using an ABI PRISM 3730 genetic analyser (Applied Biosystems; Thermo Fisher Scientific, Inc.), and then sequence traces were analysed with Mutation Surveyor (Softgenetics, PA). The mutation was confirmed in the family members by the same procedure. Multiple sequence alignments were performed using ClustalW2 with the default setting (http://www.ebi.ac.uk/Tools/clustalw2/). Protein structures were determined by SMART (http://smart.emblheidelberg.de). The variation in the 3D structure of the protein caused by gene mutation was analysed using Protein Data Bank (PDB) and the homology modelling software Swiss-Model. All genomic DNA samples were collected after obtaining informed consent.
Xing D., Zhou H., Yu R., Wang L., Hu L., Li Z., & Li X. (2020). Targeted exome sequencing identified a novel USH2A mutation in a Chinese Usher syndrome family: a case report.
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6

Targeted Exome Sequencing for Mutation Identification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA of the proband was subjected to TES. Filtered candidate variants identi ed by an Illumina HiSeq 2000 sequencer were con rmed by Sanger sequencing. The coding exons containing the detected mutations were ampli ed using Ex Tag DNA polymerase (Takara, Dalian). The puri ed PCR samples were sequenced using an ABI PRISM 3730 genetic analyser (Applied Biosystems; Thermo Fisher Scienti c, Inc.), and then sequence traces were analysed with Mutation Surveyor (Softgenetics, PA). The mutation was con rmed in the family members by the same procedure. Multiple sequence alignments were performed using ClustalW2 with the default setting (http://www.ebi.ac.uk/Tools/clustalw2/). Protein structures were determined by SMART (http://smart.emblheidelberg.de). The variation in the 3D structure of the protein caused by gene mutation was analysed using Protein Data Bank (PDB) and the homology modelling software Swiss-Model. All genomic DNA samples were collected after obtaining informed consent.
Xing D., Zhou H., Yu R., Wang L., Hu L., Li Z., & Li X. (2020). Targeted exome sequencing identified a novel USH2A mutation in a Chinese Usher syndrome family: a case report.
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