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Sars cov 2 sp

Manufactured by Sino Biological
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The SARS-CoV-2 SP is a recombinant protein that corresponds to the spike (S) protein of SARS-CoV-2. This protein is a key component of the SARS-CoV-2 virus and plays a crucial role in its ability to infect host cells.

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Lab products found in correlation

Ace2 protein, by Sino Biological (1 mentions) Bovine serum albumins, by Merck Group (1 mentions) Human serum albumins, by Merck Group (1 mentions) Potassium hexacyanoferrate 2, by Merck Group (1 mentions) Rabbit monoclonal antibody mab, by Sino Biological (1 mentions) Milli q 9 water purification system, by Merck Group (1 mentions) Sulfuric acid, by Merck Group (1 mentions) Potassium hexacyanoferrate 3, by Merck Group (1 mentions) Nitric acid, by Merck Group (1 mentions) N hydroxysuccinimide, by Merck Group (1 mentions)

2 protocols using sars cov 2 sp

1

SARS-CoV-2 Spike Protein-ACE2 Binding ELISA Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human embryonic kidney cells (HEK293T) and kidney epithelial cells (VeroE6
and Vero cells (ATCC, CCL-81)) from African green monkeys were cultured in
Dulbecco’s modified Eagle’s medium (HEK293T, VeroE6) or Minimum Essential Medium
(MEM.; Vero). HEK293T expressing ACE2 (293TACE2) was obtained from
GeneCopoeia Inc, Rockville, MD. All cell lines were supplemented with 10% fetal
bovine serum (F.B.S.), 1X L-Glutamine and 1% penicillin and streptomycin. The
rabbit polyclonal antibody against SARS-CoV-2 SP (Cat# 40150-R007) and ACE2
protein (Cat# 40592-T62) were obtained from Sino Biological and anti-HIVp24
monoclonal antibody was described previously [34 (link), 35 (link)]. The HIV-1 p24 ELISA Kit was obtained
from the AIDS Vaccine Program of the Frederick Cancer Research and Development
Center. SARS-CoV-2 SP-ACE2 binding ELISA kit (Cat# COV-SACE2-1) was purchased
from RayBio. Anti-SARS-CoV-2 neutralizing Antibody (nAb) Human IgG1(SAD-535) was
purchased from ACRO Biosystems. AH0109 was described previously [36 (link)].
Ao Z., Chan M., Ouyang M.J., Olukitibi T.A., Mahmoudi M., Kobasa D, & Yao X. (2021). Identification and evaluation of the inhibitory effect of Prunella vulgaris extract on SARS-coronavirus 2 virus entry. PLoS ONE, 16(6), e0251649.
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2

SARS-CoV-2 Spike Protein Binding Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sulfuric acid (95–98%, H2SO4), nitric acid (63%, HNO3), N-Hydroxysuccinimide (NHS), (C4H5NO3), bovine serum albumins (≥ 98%, BSA), human serum albumins (≥ 98%, HSA), potassium hexacyanoferrate (II) (≥ 99%, K4[Fe(CN)6]), potassium hexacyanoferrate (III) (≥ 99%, K3[Fe(CN)6]), EDA (≥ 99%, C2H8N2), blood plasma (BP) and saliva from human were purchased from Sigma Aldrich (St. Louis, MO, USA). Dulbecco’s phosphate buffer solution (PBS, pH 7.4) without calcium and magnesium chloride and EDC were bought from Thermo Fisher Scientific (Waltham, Massachusetts, USA). Flexible GCF (99.8%) with a thickness of 0.35 mm was purchased from Goodfellow Cambridge (Huntingdon, UK). Recombinant monoclonal SARS-CoV-2 SPike S1 antibody (anti-SARS-CoV-2) from Rabbit monoclonal antibody (MAb), SARS-CoV-2 SP, and SARS-CoV SP were purchased from Sino Biological (UK). Ultrapure water was prepared by a Milli-Q® IX water purification system (Sigma Aldrich). N2 of high purity (> 99.99%) was provided by SIAD, Bergamo, Italy).
Adeel M., Asif K., Canzonieri V., Barai H.R., Rahman M.M., Daniele S, & Rizzolio F. (2022). Controlled, partially exfoliated, self-supported functionalized flexible graphitic carbon foil for ultrasensitive detection of SARS-CoV-2 spike protein. Sensors and Actuators. B, Chemical, 359, 131591.
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