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Palb2

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PALB2 is a laboratory product that serves a core biological function. It is a protein that plays a crucial role in the DNA repair process. This product is intended for research use only and its specific applications should not be extrapolated.

Automatically generated - may contain errors

Lab products found in correlation

Rad51, by Santa Cruz Biotechnology (3 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Mre11, by Novus Biologicals (1 mentions) Fancd2, by Novus Biologicals (1 mentions) Rad52, by Santa Cruz Biotechnology (1 mentions) Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (1 mentions) Rnf168, by Merck Group (1 mentions) Flag tag (flag), by Cell Signaling Technology (1 mentions) Phospho chk1, by Cell Signaling Technology (1 mentions) Pold3, by Fortis Life Sciences (1 mentions) Brca2, by Fortis Life Sciences (1 mentions) Rad18, by Fortis Life Sciences (1 mentions) α tubulin, by Merck Group (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions) Brca2, by Abcam (1 mentions) Vinculin, by Merck Group (1 mentions) Histone h3, by Abcam (1 mentions) Ubiquityl pcna lys164, by Cell Signaling Technology (1 mentions) Ph2ax, by Merck Group (1 mentions) Horseradish peroxidase, by Cell Signaling Technology (1 mentions)

4 protocols using palb2

1

Multiprotein DNA Repair Complex Characterization

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Actin (Santa Cruz, sc-1616); BLM (Bethyl, A300-110A); RMI1 (Bethyl, A300-631A); TOP3A (ProteinTech, 14525-1); ATR (Santa Cruz, sc-1887); Chk1 (Santa Cruz, sc-8408); PolD3 (Abnova, H00010714-M01); PolD1 (Bethyl, A304-005A); PolH (Bethyl, A301-231A); BRCA1 (EMD/Calbiochem, OP92); BRCA2 (EMD/Calbiochem, OP95); PALB2 (Bethyl, IHC-00251); Rad51 (Santa Cruz, sc-8349); Rad52 (Santa Cruz, sc-365341); Mre11 (Novus, NB100-142); Smc5 (Bethyl, A300-236A); Smc6 (Bethyl, A300-237A); FANCD2 (Novus, 100-182); RNas H1 (ProteinTech, 15606-1); mCherry (GeneTex,GTX128508), GAPDH (Cell Signaling, #2118). Myc (Santa Cruz, sc-40). Antibodies recognizing FANCM, FAAP24, MHF1, and MHF2 are generously provided by Dr. Xiaodong Wang. The antibody recognizing CtIP was generously provided by Richard Baer.
Pan X., Chen Y., Biju B., Ahmed N., Kong J., Goldenberg M., Huang J., Mohan N., Klosek S., Parsa K., Guh C.Y., Lu R., Pickett H.A., Chu H.P, & Zhang D. (2019). FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops. Scientific Reports, 9, 19110.
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2

Western Blot Analysis of DNA Damage Response Proteins

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Nuclear extracts were obtained using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Scientific) and whole cell extracts were generated using RIPA buffer with protease and phosphatase inhibitors added. Proteins were separated by sodium dodecyl sulphate (SDS)-polyacrylamide gelelectrophoresis and transferred to a polyvinylidene fluoride (PVDF) membrane. Membranes were blocked with 5% nonfat milk in phosphate-buffered saline tween 20 (PBST) at room temperature for 1 h. Primary antibodies were incubated overnight at 4° and horseradish peroxidase (HRP)-conjugated secondary antibodies were incubated for 1 h at room temperature. The following primary antibodies were used: BRCA1 (EMD Millipore, catalog# OP92), RNF168 (EMD Millipore, #06-1130-I), Tubulin (Cell Signaling, catalog# 2148), GFP (Santa Cruz Biotechnology, catalog# sc-9996), RFP (ChromoTek, catalog# 6g6-20), FLAG (Cell Signaling, catalog# 14793 and Sigma Aldrich, catalog# F1804), phospho-Chk1 (Cell Signaling, catalog# 2344), Chk1 (Cell Signaling, catalog# 2360), POLD3 (Bethyl Laboratories, A301-244A), PALB2 (Bethyl Laboratories, catalog# A301-246A), BRCA2 (Bethyl Laboratories, catalog# A303-435A), RAD51 (Santa Cruz Biotechnology, catalog# sc-8349), RAD18 (Bethyl Laboratories, catalog# A301-340A), 53BP1 (Cell Signaling, catalog# 4908).
Krais J.J, & Johnson N. (2020). Ectopic RNF168 expression promotes break-induced replication-like DNA synthesis at stalled replication forks. Nucleic Acids Research, 48(8), 4298-4308.
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3

Immunoblot Analysis of DNA Repair Proteins

Cited 2 times
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The immunoblots were done as described previously (Lee et al., 2010 (link); Moskwa et al., 2011 (link)) with BRCA1 (#OP92; Calbiochem, Billerica, MA), BRCA2 (#ab16825-100; Abcam, Cambridge, MA), RAD51 (#sc-8349; Santa Cruz), MDC1 (#sc-27737; Santa Cruz), PalB2 (#A301-246A; Bethyl, Montgomery, TX), BRIP1 (#4578S; Cell Signaling), Exo1 (#sc-19941; Santa Cruz), H2AX (#2595S; Cell Signaling), and γ-H2AX (#9718S; Cell Signaling) antibodies and α-tubulin (#T5168; Sigma, St. Louis, MO) antibodies.
Choi Y.E., Pan Y., Park E., Konstantinopoulos P., De S., D'Andrea A, & Chowdhury D. (2014). MicroRNAs down-regulate homologous recombination in the G1 phase of cycling cells to maintain genomic stability. eLife, 3, e02445.
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4

Western Blot Analysis of DNA Repair Proteins

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Primary antibodies against p53 (Cell Signaling Technology, 9282), pRb (BD Pharmingen, 554136), FancD2 (Santa Cruz Biotechnology, FI17, sc-20022 or Abcam, ab2187), FancI (Santa Cruz Biotechnology, A-7, sc-271316), Phospho-Ser556 FancI and Phospho-Ser565-FancI (gifts from Ronald Cheung, Toshiyasu Taniguchi lab), pH2AX (Millipore, 05–636), Rad51 (Cosmo Bio Co. Ltd, 70–001), Palb2 (Bethyl Laboratories, A301-246A), Palb2 (a gift from Bing Xia, Rutgers Cancer Institute), Phospho-ATR (Ser428) (Cell Signaling Technology, 2853), Phospho-Chk1 (Ser345) (133D3) (Cell Signaling Technology, 2348), ATR (Cell Signaling Technology, 2790), CHK1 (2G1D5, Cell Signaling Technology, 2360), PCNA (PC10) (Cell Signaling Technology, 2586), Ubiquityl-PCNA (Lys164) (Cell Signaling Technology, 13439), UHRF1 (Santa Cruz Biotechnology, H-8, sc-373750), USP1 (C-term; a gift from Tony Huang, NYU School of Medicine), Cyclin A (in-house, Clurman lab), Beta actin (GeneTex, GTX110564 or Cell Signaling Technology, 5125), Vinculin (Sigma, V9131), and Histone H3 (Abcam, ab1791), GAPDH (14C10, Cell Signaling Technology, 2118)were used. Secondary antibodies were conjugated with horse radish peroxidase (mouse or rabbit, Cell Signaling Technology) or appropriate Alexa Fluor (Molecular Probes) were used.
Khanal S, & Galloway D.A. (2019). High-risk human papillomavirus oncogenes disrupt the Fanconi anemia DNA repair pathway by impairing localization and de-ubiquitination of FancD2. PLoS Pathogens, 15(2), e1007442.
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