Human cardiac troponin 1 elisa kit

The concentrations of serum D-dimer, C-reactive protein, ferritin, cardiac troponin I and interleukin 6 were determined with a human D-dimer ELISA kit, a human CRP ELISA kit, a human ferritin ELISA kit, a human cardiac troponin I ELISA kit and an IL-6 ELISA kit (Abcam, Cambridge, UK) measured on a PHOmo microplate reader (Autobio Diagnostics, Zhengzhou, China) according to the manufacturer's instructions. For hospitalized patients, day 1, day 7, day 14 and day 28 samples were used, while for ambulatory patients, day 1, day 14 and day 28 were used. The detection limits for D-dimer, C-reactive protein, ferritin, cardiac troponin I and interleukin 6 were 2.36 ng/mL, 5.36 pg/mL, 5 ng/mL, 4.4 pg/mL and 3 pg/mL respectively.

Cell-culture supernatant was collected into a low-binding Nunc™ 96-well polypropylene storage microplate (Thermo Fisher Scientific). We centrifuged the plate at 2000 ×g for 10 min to remove cell debris, then transferred all supernatant to a new storage plate of the same type and stored the supernatant at -20 °C until use. We employed a customized bead-based multiplex assay according to the manufacturer’s protocol (BioRad, Hercules, CA, USA) to measure IL-8, GM-SCF, IFN-γ, and TNF-α inside the supernatant. Soluble Troponin I and soluble MICA (sMICA) were measured separately using a human cardiac Troponin I ELISA kit (Abcam) and a MICA human ELISA kit (Invitrogen, Thermo Fisher Scientific), respectively, according to the manufacturers’ protocol and a Tecan Infinite M1000 Pro plate reader (Tecan, Männedorf, Switzerland).