Anti gfp monoclonal antibody

Manufactured by BD

Sourced in Canada

The Anti-GFP monoclonal antibody is a laboratory reagent designed to detect and bind to the Green Fluorescent Protein (GFP) molecule. This antibody can be used in various techniques, such as Western blotting, immunoprecipitation, and immunocytochemistry, to identify and quantify the presence of GFP-tagged proteins in samples.

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Lab products found in correlation

Monoclonal rabbit, by Cell Signaling Technology (2 mentions) Rhodamine phalloidin, by Thermo Fisher Scientific (2 mentions) Monoclonal anti cdc42 antibodies, by BD (2 mentions) Monoclonal rat, by Roche (2 mentions) Anti ha, by Abcam (1 mentions) Hrp conjugated anti rabbit or anti mouse igg, by Jackson ImmunoResearch (1 mentions) Alexa 594, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-GFP JL-8 monoclonal antibody Anti-GFP antibody Anti-GFP

3 protocols using anti gfp monoclonal antibody

Integrin Activation and Signaling Assay

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Rhodamine–phalloidin (for actin staining) was from Invitrogen (Carlsbad, CA); anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH), anti-HA, phospho-paxillin (Y118), monoclonal anti-Cdc42 antibodies, anti-GFP monoclonal antibody, active 9EG7 and total Hmβ1-1 CD29 β1-integrin, and monoclonal paxillin antibodies were from BD Biosciences (Palo Alto, CA); rat immunoglobulin G (IgG) a,κ and hamster IgG isotype controls were from Biolegend (San Diego, CA). Monoclonal rat from Roche Molecular Biochemicals (Indianapolis, IN) or monoclonal rabbit (from Cell Signaling Technology, Beverly, MA) anti-HA antibody was used for cell staining; antibodies against mouse FAK, phospho-FAK (Y397), vinculin, and α-tubulin were from Abcam (Cambridge, MA). Mouse monoclonal pan-arrestin F4C1 antibody recognizing epitope DGVVLVD in the N-domain was a generous gift of L. A. Donoso (Wills Eye Hospital, Philadelphia, PA). Arrestins were detected with arrestin-2-specific (1:6000; Mundell et al., 1999 (link)) or arrestin-3-specific (1:700; Orsini and Benovic, 1998 (link)) affinity-purified rabbit polyclonal antibodies. Total integrin antibody M-106 for Western blot was from Santa Cruz Biotechnology (Santa Cruz, CA).

Cleghorn W.M., Branch K.M., Kook S., Arnette C., Bulus N., Zent R., Kaverina I., Gurevich E.V., Weaver A.M, & Gurevich V.V. (2015). Arrestins regulate cell spreading and motility via focal adhesion dynamics. Molecular Biology of the Cell, 26(4), 622-635.

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Immunofluorescence Staining Reagents and Antibodies

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Rhodamine-phalloidin (for actin staining) was from Invitrogen (Carlsbad, CA); anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), anti-HA, monoclonal anti-Cdc42 antibodies, anti-GFP monoclonal antibody, and monoclonal paxillin antibodies were from BD Biosciences (Palo Alto, CA); monoclonal rat from Roche Molecular Biochemicals (Indianapolis, IN) or monoclonal rabbit (from Cell Signaling Technology) anti-HA antibodies were used for cell staining; antibodies against mouse FAK were from Abcam (Cambridge, MA). Pan-arrestin mouse monoclonal F4C1 antibody [32 (link)] was a gift of Dr. L. A. Donoso. Monoclonal RhoA antibody was a part of the Rho activation pulldown kit (Cytoskeleton, Denver, CO). Anti-Rac1 mouse monoclonal antibody was from Cytoskeleton (Denver, CO).

Cleghorn W.M., Bulus N., Kook S., Gurevich V.V., Zent R, & Gurevich E.V. (2017). Non-visual arrestins regulate the focal adhesion formation via small GTPases RhoA and Rac1 independently of GPCRs. Cellular signalling, 42, 259-269.

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Detecting Dictyostelium Ras Proteins

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Anti-pan Ras mouse monoclonal antibody from EMD Millipore (Billerica, MA) was used to detect D. discoideum Ras proteins. Anti-GFP monoclonal antibody was from BD Biosciences (San Jose, CA). Anti-GST monoclonal antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). HRP-conjugated anti-mouse or anti-rabbit IgG was obtained from Jackson ImmunoResearch (West Grove, PA). Alexa 594 was from Invitrogen (Carlsbad, CA). pHrodo was from Thermo Fisher Scientific (Waltham, MA).

Xu X., Pots H., Gilsbach B.K., Parsons D., Veltman D.M., Ramachandra S.G., Li H., Kortholt A, & Jin T. (2022). C2GAP2 is a common regulator of Ras signaling for chemotaxis, phagocytosis, and macropinocytosis. Frontiers in Immunology, 13, 1075386.

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