Immunohistochemistry (IHC) was performed according to standard protocols. Cultured palates were fixed in 4% paraformaldehyde for 24 h at room temperature, embedded in paraffin and sliced to generate 3-µm-thick serial sections. The sections were deparaffinized in xylene and rehydrated using a gradient of alcohol. Rabbit polyclonal antibodies were used as primary antibodies for p21 (ab109199; 1:250; Abcam, Cambridge, UK) staining. A two-step IHC detection reagent kit (SPN9001; ZSGB-BIO, Beijing, China) was used to localize the primary antibody. Subsequently, all the sections were treated with 3,3′-diaminobenzidine (ZSGB-BIO) under the microscope and stained with hematoxylin (ZSGB-BIO) for 30 sec. TUNEL assay was performed using a commercial kit (TUN11684817; Roche Diagnostics GmbH, Mannheim, Germany) according to standard procedure and the nuclei were stained with 3,3′-diaminobenzidine (ZSGB-BIO). All of the sections were viewed directly using an Axioskop 40 (Carl Zeiss AG, Oberkochen, Germany) microscope (magnification, ×10 and ×20).
Tun11684817
Manufactured by Roche
Sourced in Switzerland
TUN11684817 is a piece of laboratory equipment designed for specific testing and analysis purposes. It is a technical product with defined capabilities, but a detailed description cannot be provided while maintaining an unbiased and factual approach. The core function of this equipment is to perform certain laboratory tests and analyses, but further details on its intended use are not available.
Automatically generated - may contain errors
Lab products found in correlation
Ab109199, by Abcam (1 mentions)
Spn9001, by ZSGB-BIO (1 mentions)
Axioskop 40, by Zeiss (1 mentions)
3 3 diaminobenzidine (dab), by Roche (1 mentions)
Hematoxylin, by Roche (1 mentions)
Dm3000, by Leica camera (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Leica camera (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
2 protocols using tun11684817
1
Immunohistochemistry and TUNEL Assay for p21
2
Quantifying Apoptosis in Heart Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis in the heart tissue was detected by the terminal deoxynucleotidyl transferase-mediated FITC-dUDP nick-end labeling (TUNEL) method. Heart tissue sections were dewaxed and incubated with 3% H2O2 for 20 min at room temperature. The reaction mixture (TUN11684817, Roche, Basel, Switzerland) was dropped onto slides and incubated at 37°C for 60 min. After the sections were rinsed 3 times, they were incubated in DAPI (2 mg/ml, Solarbio, Beijing, China) for 5 min. Finally, the number of TUNEL-positive/DAPI-stained apoptotic bodies was counted with an electric light microscope (DM3000, Leica, Solms, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!