LC-MS-grade methanol, 2-propanol, water, formic acid and HPLCgrade chloroform was purchased from Biosolve (Valkenswaard, The Netherlands). LC-MS grade ammonium formate and sodium hydroxide were from Sigma-Aldrich Chemie GmbH (St Louis, USA), butanol and hydrochloric acid were from Merck Millipore (Billerica, USA). C18-lysoSM and C18-lysoGb3 were from Avanti (Albaster, USA). 13 C 5 -sphinganine, 13 C 5 -sphingosine, 13 C 5 -GlcSph and 13 C 5 -lysoGb3 were synthesized at the Department of Bio-organic Synthesis of the Leiden Institute of Chemistry, Leiden University [10] . The label was incorporated in C5, 6, 7, 8, and 9 of the sphingosine backbone (see Fig. 1). C18-sphingosine, C18-sphinganine, C18-GlcSph, C18lactosylsphingosine and C17-lysoSM were obtained from Avanti. C17-dihydroceramide (C17-dhCer) was synthesized in house (see Scheme 1); C17-sphinganine, C18-ceramide (Cer), C18glucosylceramide (GlcCer) and Lactosylceramide (LacCer) were purchased from Avanti and C18-globotriaosylceramide (Gb3) from Matreya (State College, USA).
2 propanol
Manufactured by Biosolve
Sourced in Netherlands
2-propanol, also known as isopropyl alcohol or isopropanol, is a clear, colorless, and volatile liquid. It has a molecular formula of C₃H₈O and a molar mass of 60.10 g/mol. 2-propanol is a common organic solvent used in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Biosolve (4 mentions)
Ammonium formate, by Merck Group (4 mentions)
Formic acid, by Biosolve (3 mentions)
Lc ms grade ammonium formate, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Lc ms grade methanol, by Biosolve (2 mentions)
Hplc grade chloroform, by Biosolve (2 mentions)
Synthetic lipid standards, by Avanti Polar Lipids (2 mentions)
Chloroform, by Merck Group (2 mentions)
Butylated hydroxytoluene bht, by Merck Group (2 mentions)
Acetonitrile, by Biosolve (2 mentions)
N hexane, by Biosolve (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Ammonium hydroxide solution, by Merck Group (1 mentions)
Vm100, by Arkema (1 mentions)
Synergy system, by Merck Group (1 mentions)
Ethanol absolute for analysis, by Merck Group (1 mentions)
3 aminopropyltriethoxysilane, by Merck Group (1 mentions)
Tetraethyl orthosilicate, by Merck Group (1 mentions)
Polyvinylpyrrolidone, by Merck Group (1 mentions)
13 protocols using 2 propanol
1
Comprehensive Lipidomics Profiling Protocol
2
Synthesis and Characterization of Polymer Composites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PMMA was purchased from Arkema (VM100, i.e., a PMMA-co-EA polymer,
ρ = 1.18 g cm–3) (La Garenne-Colombes, France).
Absolute ethanol for analysis was purchased from Merck (Darmstad,
Germany). Absolute tetrahydrofuran (THF), 2-propanol (99.8%), and
chloroform (99.9%) were purchased from Biosolve (Valkenswaard, The
Netherlands). Monoglycidyl ether-terminated poly(dimethylsilane) (PDMS-G)
(Mw = 1000 g mol–1),
ammonium hydroxide solution (28–30%), (3-aminopropyl)triethoxysilane
(APTES, ≥98%), sodium borohydride purum (NaBH4,
p.a., ≥96%, gas-volumetric), polyvinylpyrrolidone with a molar
mass of 40,000 g mol–1 (PVP40), and tetraethyl orthosilicate
(TEOS, ≥99.0%) were purchased from Sigma-Aldrich (St. Louis,
MO, USA). Milli-Q water was produced by a Millipore Synergy system
(Billerica, MA, USA). Unless otherwise mentioned, all other chemicals
were used as received.
ρ = 1.18 g cm–3) (La Garenne-Colombes, France).
Absolute ethanol for analysis was purchased from Merck (Darmstad,
Germany). Absolute tetrahydrofuran (THF), 2-propanol (99.8%), and
chloroform (99.9%) were purchased from Biosolve (Valkenswaard, The
Netherlands). Monoglycidyl ether-terminated poly(dimethylsilane) (PDMS-G)
(Mw = 1000 g mol–1),
ammonium hydroxide solution (28–30%), (3-aminopropyl)triethoxysilane
(APTES, ≥98%), sodium borohydride purum (NaBH4,
p.a., ≥96%, gas-volumetric), polyvinylpyrrolidone with a molar
mass of 40,000 g mol–1 (PVP40), and tetraethyl orthosilicate
(TEOS, ≥99.0%) were purchased from Sigma-Aldrich (St. Louis,
MO, USA). Milli-Q water was produced by a Millipore Synergy system
(Billerica, MA, USA). Unless otherwise mentioned, all other chemicals
were used as received.
3
Lipidomic Analysis of Synthetic Standards
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methanol, 2‐propanol, and water were purchased from Biosolve BV (Valkenswaard, Netherlands). Chloroform was from Rathburn Chemicals (Walkerburn, UK). Ammonium formate and norharmane were from Sigma‐Aldrich (Buchs, Switzerland). All solvents and chemicals were HPLC grade. Synthetic lipid standards were purchased from Avanti Polar Lipids (Alabaster, USA).
4
Analytical Workflow for Sample Purification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All solvents and chemicals were purchased at highest commercially available purity and used without further purification. Methanol, 2-propanol and formic acid (all ULC/MS grade) were from Biosolve (Valkenswaard, Netherlands), whereas chloroform and ammonium formate were from Merck KGaA (Darmstadt, Germany).
5
Lipidomic Analysis of Biological Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ULC-MS grade acetonitrile, ethanol, 2-propanol, methanol and ultra-pure water were purchased from Biosolve (Chimie SARL, Dieuze, France; BV, Valkenswaard, Netherlands). Chloroform was purchased from Carlo Erba (Milan, Italy). NH4COOH, NH4F, and butylated hydroxytoluene (BHT) were purchased from Sigma Aldrich (Milan, Italy). The Q-TOF calibration solution was prepared in acetonitrile from Agilent Technologies Tuning mix (HP0321 solution, Agilent Technologies, Santa Clara, USA).
N-palmitoyl-d31-D-erythro-sphingosine (d31-Cer[NS]34:1, MW 569.1), N-[26-oleoyloxy(d9) hexacosanoyl]-d -erythro-sphingosine (Cer[EOS] (d18:1/26:0/18:1(d9), MW 967.6) and N-[26-oleoyloxy hexacosanoyl]-d -erythro-sphingosine (Cer[EOS] (d18:1/26:0/18:1), MW 958.6) were purchased from Avanti Polar Lipids (Alabaster, USA). Deuterated cholesterol sulfate sodium salt (d7-CHS, MW 495.3) and Hexadecanoic-9,9,10,10,11,11,12,12,13,13,14,14,15,15,16,16,16-d17 acid (d17-PA, MW 273.5) were purchased from CDN Isotopes Inc., (Pointe-Claire, Canada). Stock solution of the internal standard (iSTD) mixture of deuterated compounds was prepared in 2-propanol with the following concentrations: d7-CHS 40 µM, d17-PA acid 80 µM, d31-Cer16:0 10 µM. d9-CER[EOS] and CER[EOS] mixture (2 µM each in Chloroform/methanol (2:1)) was introduced to confirm identification of O-acylceramides.
N-palmitoyl-d31-D-erythro-sphingosine (d31-Cer[NS]34:1, MW 569.1), N-[26-oleoyloxy(d9) hexacosanoyl]-
6
Naphthalene Isopropylation Reaction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The bulk scale naphthalene
isopropylation reaction was performed in the liquid phase using a
high-pressure 15 mL TOP reactor for 8 h at 200 °C and under 2
× 106 Pa of N2 gas (99.999%). A figure
of the apparatus was added to theSupporting Information (Figure S1). The reaction mixture consisted of 1.84 mmol of naphthalene
(Sigma-Aldrich, ≥99%), 4 mmol of 2-propanol (Biosolve ≥99.8%),
9 mL of cyclohexane (Sigma-Aldrich, ≥ 99.8%), and 100 mg of
the MOR catalyst. The catalyst powder is used as provided by the manufacturer
or as obtained after acid leaching, i.e., crystal size 1–3
μm and average particle size 18 μm. After 8 h of reaction,
the reactor was cooled by placing it in an ice bath. The reaction
mixture was analyzed using a gas chromatograph (Shimadzu, CP-Sil 8,
FID detector) in split injection mode (split ratio 20) using N2 carrier gas. After injection, the temperature was programmed
as follows: 5 min hold at 40 °C, heating to 280 °C at 4
°C/min, and finally a 10 min holding time. A total of 2 mmol
of n-tetradecane (TCI, ≥ 99%) was added as an internal standard
for quantitative gas chromatography (GC) analysis. Identification
of the compounds was carried out using gas chromatography–mass
spectrometry.
isopropylation reaction was performed in the liquid phase using a
high-pressure 15 mL TOP reactor for 8 h at 200 °C and under 2
× 106 Pa of N2 gas (99.999%). A figure
of the apparatus was added to the
(Sigma-Aldrich, ≥99%), 4 mmol of 2-propanol (Biosolve ≥99.8%),
9 mL of cyclohexane (Sigma-Aldrich, ≥ 99.8%), and 100 mg of
the MOR catalyst. The catalyst powder is used as provided by the manufacturer
or as obtained after acid leaching, i.e., crystal size 1–3
μm and average particle size 18 μm. After 8 h of reaction,
the reactor was cooled by placing it in an ice bath. The reaction
mixture was analyzed using a gas chromatograph (Shimadzu, CP-Sil 8,
FID detector) in split injection mode (split ratio 20) using N2 carrier gas. After injection, the temperature was programmed
as follows: 5 min hold at 40 °C, heating to 280 °C at 4
°C/min, and finally a 10 min holding time. A total of 2 mmol
of n-tetradecane (TCI, ≥ 99%) was added as an internal standard
for quantitative gas chromatography (GC) analysis. Identification
of the compounds was carried out using gas chromatography–mass
spectrometry.
7
Lipid Extraction and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methanol, 2-propanol and water were purchased from Biosolve BV (Valkenswaard, The Netherlands). Chloroform was from Rathburn Chemicals (Walkerburn, UK). Ammonium formate was from Sigma-Aldrich (Buchs, Switzerland). All solvents and chemicals were of the highest analytical grade. Synthetic lipid standards were purchased from Avanti Polar Lipids (Alabaster, AL, USA).
8
Synthesis of Lipid Standards for Gaucher's Disease
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GCase specific inhibitor (ME656) (34 (link)), 13C5-sphinganine, 13C5-sphingosine, 13C5-GlcSph, 13C5-lyso-globotriaosylceramide (LysoGb3), C17-lysosphingomyelin (LysoSM), 13C6-GlcChol, and C17-dihydroceramide (38 (link), 39 (link)) were synthesized as reported. All chemicals and reagents were obtained from Sigma-Aldrich (St Louis, USA) unless mentioned otherwise. The standards Cer (d18:1/16:0), dhCer (d18:0/16:0), GlcCer (d18:1/16:0), galactosylceramide (GalCer) (d18:1/16:0), and LacCer (d18:1/16:0) were obtained from Avanti Polar lipids (Alabaster, USA) and glucosylated cholesterol (GlcChol) from Sigma-Aldrich. LC-MS grade methanol, 2-propanol, water, formic acid, acetonitrile, and HPLC grade chloroform were purchased from Biosolve (Valkenswaard, the Netherlands). LC-MS grade ammonium formate, ammonium acetate, and sodium hydroxide from Sigma-Aldrich, and butanol and hydrochloric acid from Merck Millipore (Billerica, USA).
9
Lipid Extraction and Derivatization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acetonitrile, n-hexane, methanol, 2-propanol, and water were purchased from Biosolve BV (Valkenswaard, The Netherlands). Chloroform was from Rathburn Chemicals (Walkerburn, UK). Ammonium formate was from Sigma-Aldrich (Buchs, Switzerland). All solvents and chemicals were HPLC grade. Sulfuric acid and butylated hydroxytoluene (BHT) were from Sigma-Aldrich (Steinheim, Germany), 18O-labeled water (i.e., H218O) was from Medical Isotopes (Pelham, NH, USA). Lipid standards PE 15:0/18:1(+2H7) (1-pentadecanoyl-2-oleoyl(d7)-sn-glycero-3-phosphoethanolamine) and LPC 16:0(+2H3) (1-palmitoyl-d3-sn-glycero-3-phosphocholine) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). A FAME standard mixture, with equal amounts of FAME 16:0, 18:0, 16:1, 18:1, 18:2, 18:3n−3, 18n−6, 20:3, 20:4, 20:5, 22:4, 22:5n−3, 22:5n−6 and 22:6, was obtained from Larodan Fine Chemicals (Solna, Sweden).
10
Quantitative Lipidomics Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The solvents n-hexane, 2-propanol (iPrOH), formic acid (FA), acetonitrile (ACN), and methanol (MeOH) of ultrahigh-performance LC/MS grade were purchased from Biosolve (Valkenswaard, Netherlands). Water was produced in-house with a Barnstead GenPure System from Thermo Scientific (Waltham, MA, USA). Methyl tert-butyl ether (MTBE) of pro analysis quality was obtained from Carl Roth (Karlsruhe, Germany). 2-Butanol of extra-pure quality was purchased from Thermo Fisher Scientific (Waltham, MA, USA). 2,6-Di-tert-butyl-4-methylphenol (BHT) of gas chromatography quality and EDTA of ACS reagent grade were purchased from Sigma-Aldrich (St Louis, MO, USA). Ethyl acetate and chloroform (CHCl3) of LC grade, zinc sulfate heptahydrate of pro analysis quality, and phosphate-buffered saline (PBS) of liquid, sterile-filtered, suitable-for-cell-culture grade were obtained from Merck Chemicals (Darmstadt, Germany). Tris (hydroxymethyl)aminomethane–HCl buffer was obtained from Bioanalytic (Umkirch, Germany). Unlabeled and deuterium-labeled PUFA and eicosanoid standards were purchased from Cayman Chemicals (Ann Arbor, MI, USA); the detailed information can be found in Table S1 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!