Mouse monoclonal anti β amyloid 6e10 antibody

This was performed using PBS and human serum treated with bio-Aβ42 and plasma from AD patients. Amytrapper catheter (plus API catheter), or uncoated catheter (minus API catheter) were blocked with 0.01% BSA in PBS (Sigma) overnight at 4⁰C. BSA was removed and catheters were washed 5 times with PBS. The catheters were incubated with 40 ng of bio-Aβ42 in 350 μl PBS or 40 ng of bio-Aβ42 in 350 μl human normal serum (Innovative Research, MI) or 350 μl plasma from AD patients (Innovative Research, MI). After 2 hours incubation at room temperature, sample solutions were transferred to Elisa plate for measurement of bio-Aβ42 or Aβ. Catheters were washed with PBS for 10 times. Catheters were incubated with mouse monoclonal anti-β-Amyloid 6E10 antibody (1/2000, BioLegend) for 1 hour at room temperature. Catheters were washed 10 times with PBS and then incubated with goat anti-mouse HRP (Invitrogen) for 1 hour at room temperature. Catheters were washed for 10 times with PBS. Catheters were incubated with 350 μl of SureBlue TMB substrate (KPL) for 2 minutes at room temperature. Substrate was collected in an Eppendorf tube. 350 μl of 1N HCl was added to stop the reaction. 100 μl of mixture was transferred to a 96 well clear plate and read at 450 nm on the Elx800 plate reader.