Venous blood sampling was performed by using tubes containing EDTA. Platelet counts and MPV were automatically determined on an ADVIA120 Haematology System (Siemens, Erlangen, Germany) within 1 h after blood sampling. In early foetal demise patients, the MPV and platelet counts were analysed before ultrasound diagnosed as early foetal demise.
Advia 120 haematology system
Manufactured by Siemens
Sourced in Germany
The ADVIA 120 haematology system is a fully automated analyzer designed for measuring and analyzing various blood parameters. It provides comprehensive cell counting and differentiation capabilities for routine clinical hematology testing.
Automatically generated - may contain errors
Lab products found in correlation
10 protocols using advia 120 haematology system
1
Platelet Count and MPV in Early Foetal Demise
2
Haematological Analysis of ACP, E-PET, and NaCl
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Haematological analysis of fresh ACP®, E-PET and NaCl (control) aliquots were carried out using the ADVIA 120 haematology system (Siemens Healthcare GmbH, Erlangen, Germany).
3
Catecholamine and Cortisol Dynamics in Sheep
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To measure epinephrine and norepinephrine concentrations, continuous blood samples (6 mL) were collected at 30 s intervals into pre-chilled EDTA tubes (Becton Dickinson, Plymouth, UK) using a peristaltic pump starting -2 min and ending 10 min relative to the start of the infusion period (time 0). Additional blood samples (6 mL) were collected into tubes containing lithium heparin (Becton Dickinson) to measure cortisol and pack cell volume (PCV) concentrations. The maximum amount of blood taken per sheep was 250 mL. Blood samples were immediately placed on ice and centrifuged (2500 rpm) at 4°C for 10 min, within 10 min of collection. Plasma was stored at –80°C until analysed. Samples collected at −2, 0, 2, 4, 6, 8 and 10 min were assayed for epinephrine and norepinephrine concentrations using methodology described in Stewart et al. [19 (link)]. Briefly, 1 mL of plasma was extracted on alumina and the extracted catecholamines were separated and measured using reverse-phase HPLC. Cortisol concentrations were measured at –10, –5 and 15 min using a commercially available antibody radioimmunoassay kit (Coat-A-Count® Cortisol, Siemens; Los Angeles, CA). Packed cell volume was measured in samples taken at -5 and 5 min, using an ADVIA 120 Haematology system (Siemens Healthcare GmbH, Erlangen, Germany).
4
Platelet Count and MPV in Early Foetal Demise
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Venous blood sampling was performed by using tubes containing EDTA. Platelet counts and MPV were automatically determined on an ADVIA120 Haematology System (Siemens, Erlangen, Germany) within 1 h after blood sampling. In early foetal demise patients, the MPV and platelet counts were analysed before ultrasound diagnosed as early foetal demise.
5
ADVIA 120 Hematology Analysis
6
ADVIA 120 Hematology Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood cells were analysed on an ADVIA 120 haematology system (SIEMENS).
7
Circulating Neutrophil and Monocyte Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was obtained from mice and immediately added to K2EDTA-coated Microtainer tubes (BD, Franklin Lakes, NJ) to prevent clotting. The proportions of circulating neutrophils and monocytes within the white blood cell population was determined using the ADVIA® 120 Haematology System (Siemens Healthcare Diagnostics Inc, Tarrytown, NY).
8
Comprehensive Metabolic and Inflammatory Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The basal blood and 24-h urine samples were analysed in the local laboratory using standardised assays. Specifically, glucose, creatinine, sodium, potassium, calcium, magnesium, total cholesterol, HDL-c, triglycerides, and high-sensitive C-reactive protein (hs-CRP) were measured in serum on an Atellica CH930 chemistry analyser (Siemens Healthcare Diagnostics). LDL-c was calculated using the Friedewald formula. Serum and 24-h urinary creatinine, proteins, and albumin were measured on an Atellica CH930 chemistry analyser. Insulin was measured by chemiluminescence immunoassay on an Atellica IM1 600 analyser, and homeostatic model assessment-insulin resistance (HOMA-IR) was calculated using the following formula: glucose (mmol L−1) × insulin (mIU L−1)/22.5. HbA1C was measured on a Tosoh G8 HPLC Analyzer. Leukocyte formula and blood count were measured on an ADVIA 120 haematology system (Siemens Healthcare Diagnostics).
9
Accounting for Cell Type Proportions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The percentage of cells methylated may vary depending on the tissue - and cell types that composed that tissue - in which it is measured. In the case of whole blood, the DNA methylation measured is that of all leukocyte subtypes together (e.g. neutrophils, monocytes, etc.). Thus, the percentage of cells methylated (i.e. methylation level) in whole blood is an aggregate measure of the proportion from each blood cell types. Inter-individual differences in blood cell type proportions can thus affect blood DNA methylation levels38 (link), and should be accounted for in the analyses. In the MARTHA study, the proportions of lymphocytes, monocytes, neutrophils, eosinophils and basophils were directly measured from whole blood using the ADVIA 120 Haematology System (Siemens Healthcare Diagnostics, Deerfield, IL). Cell type proportions were not measured in the F5L family study. Instead, we used Remove Unwanted Variation (RUV) method39 (link), 40 (link) to capture the cell type proportions as described in Supplemental Data.
10
Measuring Blood Eosinophil Counts in COPD
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood eosinophil counts were measured in fresh blood samples using the ADVIA 120 haematology system (Siemens Healthineers, Erlingen, Germany) and reported in total numbers (×10 9 cells•L -1 ). We defined high versus low eosinophil counts in individuals with COPD as blood eosinophil counts of ⩾0.34×10 9 cells•L -1 versus those <0.34×10 9 cells•L -1 , as determined in our previous work on blood eosinophil counts and COPD exacerbations [16] . However, in sensitivity analyses we used blood eosinophil cut-points of 2% of all leukocytes, which has been widely used [15] , and 3.3%, determined in our previous work [16] .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!