During this study the following NO donors were used: 3-(aminopropyl)-1-hydroxy-3-isopropyl-2-oxo-1-triazene (NOC-5, 5–20 μM, Santa Cruz Biotechnology, Inc. and Enzo Life Sciences), S-nitrosoglutathione (GSNO, 100–300 μM, Santa Cruz Biotechnology, Inc.), propylamine propylamine NONOate (PAPA NONOate, 5–20 μM, Santa Cruz Biotechnology, Inc. and Enzo Life Sciences), S-nitroso-N-acetylpenicillamine (SNAP, 5–20 μM, Life Technologies) and sodium nitroprusside dehydrate (SNP, 100–300 μM, Sigma). NO concentrations are expressed as mean ± SEM and displayed in figures as box and whisker plots to indicate median value and interquartile range.
Sodium nitroprusside dehydrate
Manufactured by Merck Group
Sourced in Italy, United Kingdom
Sodium nitroprusside dehydrate is a chemical compound with the formula Na₂[Fe(CN)₅NO]·2H₂O. It is a crystalline solid that is commonly used as a laboratory reagent.
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Lab products found in correlation
Sulphanilamide, by Merck Group (1 mentions)
Naphthylethylenediamine dihydrochloride, by Merck Group (1 mentions)
Homoglucocamelinin potassium salt, by Extrasynthese (1 mentions)
Fluorescein sodium salt, by Merck Group (1 mentions)
Glucoarabinin potassium salt, by Extrasynthese (1 mentions)
Milli q system, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Ammonium hydroxide, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Phosphoric acid, by Merck Group (1 mentions)
2 2 azobis 2 amidinopropane dihydrochloride aaph, by Tokyo Chemical Industry (1 mentions)
Acetylcholine chloride, by Merck Group (1 mentions)
Nifedipine, by Merck Group (1 mentions)
Papaverine hydrochloride, by Merck Group (1 mentions)
U46619, by Bio-Techne (1 mentions)
Nω nitro l arginine methyl ester hydrochloride l name, by Merck Group (1 mentions)
Phenylephrine hydrochloride, by Merck Group (1 mentions)
Arg8 vasopressin acetate salt, by Merck Group (1 mentions)
3 protocols using sodium nitroprusside dehydrate
1
Nitric Oxide Donor Protocols
2
UPLC Analytical Reagent Preparation
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MS-grade solvents used for UPLC analysis acetonitrile (MeCN) water (H2O) and formic acid (HCOOH) were provided by Romil (Cambridge, UK); analytical-grade solvents methanol (MeOH) and ethanol (EtOH) were supplied by Sigma-Aldrich (Milan, Italy). H2O was purified by using a Milli-Q system (Millipore, Bedford, USA). Acetic acid (AA), ammonium hydroxide, naphthylethylene diamine dihydrochloride, phosphoric acid, ascorbic acid, fluorescein sodium salt, Trizma hydrocloride (Tris-HCl) monopotassium phosphate dipotassium phosphate, sodium nitroprusside dehydrate (SNP), sulphanilamide were provided by Sigma-Aldrich (Milan, Italy). 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH) were purchased from TCI Chemicals (Tokyo, Japan). Glucoarabinin potassium salt, glucocamelin potassium salt and homoglucocamelinin potassium salt were purchased from Extrasynthese (Lyion, France).
3
Wire Myography Buffer Compositions
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Two types of buffers were used for wire myography experiments, physiological salt solution (PSS) and high potassium physiological salt solution (KPSS). The composition of PSS (in mM) is sodium chloride 119, potassium chloride 4.7, magnesium sulfate heptahydrate 1.17, sodium bicarabonate 25, potassium dihydrogen orthophosphate 1.18, EDTA 0.027, D-(+)-glucose 5.5, calcium chloride dehydrate 2.5. For KPSS, sodium chloride was replaced with 123.7 mM potassium chloride. Both were prepared according to protocols developed by Mulvany [3] . (R)-(-)-Phenylephrine hydrochloride (P6126), [Arg 8 ]-Vasopressin acetate salt (V9879), acetylcholine chloride (A6625), angiotensin II human (A9525), bradykinin acetate salt (B3259), histamine (H7125), indomethacin (I7378), L-norepinephrine hydrochloride (74480), nifedipine (N7634), NΩ-nitro-L-arginine methyl ester hydrochloride (L-NAME) (N5751), papaverine hydrochloride (P3510), riluzole (R116), sodium nitroprusside dehydrate (S0501) and Substance P acetate salt hydrate (S6883) were bought from Sigma, UK. U46619 (1932) was bought from Tocris, UK. Endothelin-1 (human, porcine) (ab120471) was purchased from Abcam, UK. Serotonin (hydrochloride) (14332) was bought from Cayman, US. SQ29548 (BML-RA103) was purchased from Enzo, UK.
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