Mouse monoclonal anti-ABCB4 (clone P3-II-26) and anti-MRP2 (multidrug resistance-associated protein 2; clone M2-I-4) antibodies were purchased from Enzo Life Sciences (Villeurbanne, France); and anti-α-tubulin (clone 1E4C11) was sourced from ProteinTech (Manchester, United Kingdom). Alexa Fluor-labeled secondary antibodies, the DRAQ5 fluorescent probe and culture media came from ThermoFisher (Cergy-Pontoise, France), and peroxidase-conjugated secondary antibodies were acquired from Rockland Immunochemicals (Gilbertsville, PA, USA). The ECL-Prime detection kit was from VWR (Courtaboeuf, France). The transfection reagents Turbofect and JetPrime were purchased from Thermo-Fisher Scientific, (Saint-Herblain, France) and Ozyme (Saint-Cyr-l’Ecole, France), respectively. Ivacaftor (VX-770) came from Clinisciences (Nanterre, France). All other reagents were obtained from Sigma-Aldrich (Lyon, France).
Peroxidase conjugated secondary antibodies
Manufactured by Rockland Immunochemicals
Sourced in France
Peroxidase-conjugated secondary antibodies are lab equipment used as detection reagents in various immunoassays. They function by binding to primary antibodies and catalyzing a color-producing reaction, thereby enabling the visualization and quantification of target analytes.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor labeled secondary antibody, by Thermo Fisher Scientific (4 mentions)
Jetprime, by Thermo Fisher Scientific (2 mentions)
Draq5 fluorescent probe, by Thermo Fisher Scientific (2 mentions)
Ecl prime detection kit, by Avantor (2 mentions)
Turbofect, by Thermo Fisher Scientific (2 mentions)
Culture media, by Thermo Fisher Scientific (2 mentions)
Anti mrp2, by Enzo Life Sciences (1 mentions)
Anti α tubulin clone 1e4c11, by Proteintech (1 mentions)
Rabbit polyclonal anti c myc, by Santa Cruz Biotechnology (1 mentions)
Control sirna, by GE Healthcare (1 mentions)
Monoclonal anti β actin, by Merck Group (1 mentions)
Ecl plus detection kit, by GE Healthcare (1 mentions)
Mouse monoclonal anti gfp, by Roche (1 mentions)
Poly l lysine (pll), by Merck Group (1 mentions)
4 protocols using peroxidase conjugated secondary antibodies
1
Immunofluorescence and Western Blot Analysis of ABCB4 and MRP2
2
Monoclonal Anti-ABCB4 Antibody Protocol
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The monoclonal P3II-26 anti-ABCB4 antibody was obtained from Enzo Life Science (Villeurbanne, France). Rabbit polyclonal anti-EBP50 was from Thermo Fisher Scientific (Rockford, IL). Rabbit polyclonal anti-Flag was obtained from Abcam (Cambridge, UK) and the monoclonal anti-β actin from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Rabbit polyclonal anti-c-myc was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Alexa Fluor-labeled secondary antibodies, DRAQ5 fluorescent probe and culture media were from Invitrogen-Life Technologies (Cergy-Pontoise, France) and peroxidase-conjugated secondary antibodies were from Rockland Immunochemicals (Gilbersville, PA). The siRNA-EBP50 duplex, the control siRNA and the ECL-Plus detection kit were from GE Healthcare France (Orsay, France). The transfection reagents Turbofect and Fugene HD were purchased from Fermentas France (Villebon-sur-Yvette, France) and Promega (Charbonnières, France), respectively. All other reagents were obtained from Sigma-Aldrich.
3
Proteomic analysis of ABCB4 regulation
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The mouse monoclonal P3II-26 anti-ABCB4 antibody was obtained from Enzo Life Sciences (Villeurbanne, France). Rabbit polyclonal anti-MRCKα and anti-MRLC were from Euromedex (Souffelweyersheim, France). Monoclonal anti-myc and anti-α-tubulin antibodies were from ThermoFisher (Cergy-Pontoise, France) and ProteinTech (Manchester, United Kingdom), respectively. The mouse monoclonal anti-GFP was from Roche (Meylan, France). Alexa Fluor-labeled secondary antibodies, DRAQ5 fluorescent probes, and culture media were from ThermoFisher (Cergy-Pontoise, France), and peroxidase-conjugated secondary antibodies were from Rockland Immunochemicals (Gilbertsville, PA). The control non-specific siRNA “ON-TARGETplus Non-targeting Control Pool”, MRCKα siRNA “ON-TARGETplus Human CDC42BPA siRNA SMARTpool”, Myl12a siRNA “ONTARGETplus Human Myl12a siRNA SMARTpool”, and Myl12b siRNA “ON-TARGETplus Human Myl12b siRNA SMARTpool” were from Dharmacon-GE Healthcare (Fontenay-sous-Bois, France). The ECL-Prime detection kit was from VWR (Courtaboeuf, France). The transfection reagents Turbofect and JetPrime were purchased from ThermoFisherScientific, (Saint-Herblain, France) and Ozyme (Saint-Cyr-l’Ecole, France), respectively. Chelerythrine chloride was obtained from Enzo Life Science (Villeurbanne, France).
4
Immunofluorescence and Immunoblotting of ABCB4
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Indirect immunofluorescence was performed on cells grown on glass coverslips after fixation with methanol at -20°C, as described (Delaunay et al., 2009) . In the case of HEK293 cells, coverslips were pre-coated with poly-L-lysine (Sigma-Aldrich, Saint-Quentin Fallavier, France). The monoclonal P3II-26 anti-ABCB4 was obtained from Enzo Life Sciences (Villeurbanne, France) and Alexa Fluor-labeled secondary antibodies were from Invitrogen-Life Technologies. Electrophoresis and immunoblotting were performed as described (Delaunay et al., 2009) using the monoclonal P3II-26 anti-ABCB4 antibody, and peroxidaseconjugated secondary antibodies from Rockland Immunochemicals (Gilbertsville, PA).
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