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CYP3A is a lab equipment product that serves as a cytochrome P450 enzyme. It plays a core role in the metabolism of various drugs and other xenobiotics.

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Lab products found in correlation

Cyp2e1, by Santa Cruz Biotechnology (2 mentions) Flotillin 1, by Santa Cruz Biotechnology (1 mentions) P egfr, by Cell Signaling Technology (1 mentions) P src, by Cell Signaling Technology (1 mentions) Oxylipin standards, by Cayman Chemical (1 mentions) P fak, by Cell Signaling Technology (1 mentions) Actin, by Santa Cruz Biotechnology (1 mentions) Protein assay system, by Bio-Rad (1 mentions) Cyp1a1, by Santa Cruz Biotechnology (1 mentions) Immun star western chemiluminescent kit, by Bio-Rad (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Sds page, by Bio-Rad (1 mentions)

3 protocols using cyp3a

1

Oxylipin Analysis and Signaling Pathway

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All chemicals and solvents were used as purchased. Oxylipin standards were purchased from Cayman Chemicals (Ann Arbor, MI). Antibodies used for western blotting and immunohistochemical staining were: FAK, p-FAK, Src, p-Src, EGFR, and p-EGFR obtained from Cell Signaling Technology (Beverly, MA); and CYP2B, CYP2C9/19, CYP2J, CYP3A, and flotillin-1 purchased from Santa Cruz (Santa Cruz, CA).
Apaya M.K., Shiau J.Y., Liao G.S., Liang Y.J., Chen C.W., Yang H.C., Chu C.H., Yu J.C, & Shyur L.F. (2019). Integrated omics-based pathway analyses uncover CYP epoxygenase-associated networks as theranostic targets for metastatic triple negative breast cancer. Journal of Experimental & Clinical Cancer Research : CR, 38, 187.
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2

Protein Expression Analysis by Western Blot

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Tissue and cell proteins were extracted as reported previously17 (link) and concentration was determined using the Bio-Rad protein assay system (Bio-Rad, Munich, Germany). Fifty micrograms total protein extracts were separated on 8%−12% sodium dodecyl sulfate polyacrylamide gel electrophoresis and analyzed by Western blotting using antibodies against HO-1 (Stressgen, Victoria, Canada), L- and H-ferritin (kindly provided by Sonia Levi), ferroportin (Fpn; Alpha Diagnostic Intl. Inc, San Antonio, TX), CYP1A1, CYP3A, CYP2E1, and actin (Santa Cruz Biotechnology, Inc., Dallas, TX).
Vinchi F., Ingoglia G., Chiabrando D., Mercurio S., Turco E., Silengo L., Altruda F, & Tolosano E. (2014). Heme Exporter FLVCR1a Regulates Heme Synthesis and Degradation and Controls Activity of Cytochromes P450. Gastroenterology, 146(5), 1325-1338.
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3

Quantitative Western Blot Analysis of Hepatic CYPs

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Total liver microsomal protein concentrations in samples were determined with the Bradford method [20 (link)] using bovine serum albumin (BSA) as standard. Total cellular proteins (50 μg) were separated by 12% precast sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE: Bio-Rad). The gel was blotted onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad) and incubated with the primary polyclonal antibodies: CYP1A (Biosense Laboratories 1:500), CYP3A (1:2000), and CYP2E1, CYP2B1/2B2, CYP26 (Santa Cruz Biotechnologies, 1:500) antisera. After washing, membranes were incubated with peroxidase conjugated goat anti-rabbit/mouse antibodies (GAR/GAM-HRP; Bio-Rad) diluted 1:3000, developed using an Immun-Star Western Chemiluminescent Kit (Bio-Rad) and visualized with Eastman Kodak Company’s Molecular Imaging Systems (Rochester, NY, USA).
Arukwe A., Røsbak R., Adeogun A.O., Langberg H.A., Venter A., Myburgh J., Botha C., Benedetti M, & Regoli F. (2015). Biotransformation and Oxidative Stress Responses in Captive Nile Crocodile (Crocodylus niloticus) Exposed to Organic Contaminants from the Natural Environment in South Africa. PLoS ONE, 10(6), e0130002.
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